Abstract
Evidence for complement utilization in middle ear fluids (MEF) from patients with otitis media with effusion was sought. It was found that cleavage products of C3, C4 and Factor B could be demonstrated immunochemically in MEF, and that native C3 was present in much lower concentrations than other proteins, relative to their serum concentrations. Haemolytic assays for C1-C5 showed that early complement components are inactivated in MEF. Potential mechanisms for complement utilization in MEF are discussed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bluestone C. D., Paradise J. L., Beery Q. C. Physiology of the eustachian tube in the pathogenesis and management of middle ear effusions. Laryngoscope. 1972 Sep;82(9):1654–1670. doi: 10.1288/00005537-197209000-00009. [DOI] [PubMed] [Google Scholar]
- Bryan M. P., Bryan W. T. Cytologic and immunologic response revealed in middle ear effusions. Ann Otol Rhinol Laryngol. 1976 Mar-Apr;85(2 Suppl 25 Pt 2):238–244. doi: 10.1177/00034894760850S244. [DOI] [PubMed] [Google Scholar]
- Howie V. M., Ploussard J. H. The "in vivo sensitivity test"--bacteriology of middle ear exudate, during antimicrobial therapy in otitis media. Pediatrics. 1969 Dec;44(6):940–944. [PubMed] [Google Scholar]
- LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
- Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
- Molenaar J. L., Müller M. A., Engelfriet C. P., Pondman K. W. Changes in antigenic properties of human C3 upon activation and conversion by trypsin. J Immunol. 1974 Apr;112(4):1444–1451. [PubMed] [Google Scholar]
- Nelson R. A., Jr, Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum. Immunochemistry. 1966 Mar;3(2):111–135. doi: 10.1016/0019-2791(66)90292-8. [DOI] [PubMed] [Google Scholar]
- SENTURIA B. H., CARR C. D., AHLVIN R. C. Middle ear effusions: pathologic changes of the mucoperiosteum in the expeimal. Ann Otol Rhinol Laryngol. 1962 Sep;71:632–647. doi: 10.1177/000348946207100306. [DOI] [PubMed] [Google Scholar]
- Sade J. Pathology and pathogenesis of serous otitis media. Arch Otolaryngol. 1966 Sep;84(3):297–305. doi: 10.1001/archotol.1966.00760030299007. [DOI] [PubMed] [Google Scholar]
- Senturia B. H. Classification of middle ear effusions. Ann Otol Rhinol Laryngol. 1970 Apr;79(2):358–370. doi: 10.1177/000348947007900216. [DOI] [PubMed] [Google Scholar]
- Shiraishi S., Stroud R. M. Cleavage products of C4b produced by enzymes in human serum. Immunochemistry. 1975 Dec;12(12):935–939. doi: 10.1016/0019-2791(75)90256-6. [DOI] [PubMed] [Google Scholar]
- Sjöholm A. G., Laurell A. B. Conversion of the fourth complement component studied by crossed immunoelectrophoresis. Clin Exp Immunol. 1973 Aug;14(4):515–529. [PMC free article] [PubMed] [Google Scholar]
- Stephens C. G., Williams R. C., Jr, Reed W. P. Classical and alternative complement pathway activation by pneumococci. Infect Immun. 1977 Aug;17(2):296–302. doi: 10.1128/iai.17.2.296-302.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]