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. 1979 Mar;35(3):364–375.

Antibody penetration into living cells. I. Intranuclear immunoglobulin in peripheral blood mononuclear cells in mixed connective tissue disease and systemic lupus erythematosus.

D Alarcón-Segovia, A Ruíz-Argüelles, E Fishbein
PMCID: PMC1537612  PMID: 378481

Abstract

We have shown recently (Alarcón-Segovia, Ruíz-Argüelles & Fishbein, 1978) that an IgG anti-RNP antibody obtained from a patient with mixed connective tissue disease (MCTD) can penetrate viable mononuclear cells (MNC) from normal donors via their Fc receptors. Live MNC from twelve MCTD patients incubated with goat anti-Ig antibody had intranuclear antibody with a speckled pattern in a mean of 5.5% of all MNC and 57.3% of all Fc receptor-bearing MNC. We found intranuclear immunoglobulins in all twelve patients with MCTD which were present only in cells with Fc receptors. Only three out of twenty-one patients with systemic lupus erythematosus (SLE) were found to have intranuclear antibody in a mean of 17.2% of their Fc receptor-bearing cells. Further experiments with MNC from SLE patients revealed a partial blocking of penetration of antibody via Fc receptors. MNC from ten scleroderma, ten rheumatoid arthritis patients and eleven normal controls did not have intranuclear immunoglobulin. In vivo penetration of autoantibodies into Fc receptor-bearing cells in MCTD, and probably in SLE as well, may represent an important pathogenetic mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alarcon-Segovia D., Fishbein E., Estrada-Parra S. The heterogeneity of anti-DNA antibodies in systemic lupus erythematosus and other diseases. J Rheumatol. 1975 Jun;2(2):172–177. [PubMed] [Google Scholar]
  2. Alarcon-Segovia D. Symptomatic Sjögren's syndrome in mixed connective tissue disease. J Rheumatol. 1976 Jun;3(2):191–195. [PubMed] [Google Scholar]
  3. Alarcón-Segovia D., Fishbein E., Alcalá H., Olguín-Palacios E., Estrada-Parra S. The range and specificity of antinuclear antibodies in systemic lupus erythematosus. Clin Exp Immunol. 1970 Apr;6(4):557–571. [PMC free article] [PubMed] [Google Scholar]
  4. Borthistle B. K., Kubo R. T., Brown W. R., Grey H. M. Studies on receptors for IgG on epithelial cells of the rat intestine. J Immunol. 1977 Aug;119(2):471–476. [PubMed] [Google Scholar]
  5. Cordier G., Samarut C., Revillard J. P. Changes of Fcgamma receptor-related properties induced by interaction of human lymphocytes with insoluble immune complexes. J Immunol. 1977 Dec;119(6):1943–1948. [PubMed] [Google Scholar]
  6. Díaz-Jouanen E., Rivero S. J., Llorente L., Alarcón-Segovia D. Receptor and non-receptor-bearing lymphocytes in untreated systemic lupus erythematosus. Variations with disease activity. Rev Invest Clin. 1977 Oct-Dec;29(4):265–276. [PubMed] [Google Scholar]
  7. Fröland S. S. Binding of sheep erythrocytes to human lymphocytes. A probable marker of T lymphocytes. Scand J Immunol. 1972;1(3):269–280. doi: 10.1111/j.1365-3083.1972.tb01818.x. [DOI] [PubMed] [Google Scholar]
  8. Gilliam J. N., Prystowsky S. D. Mixed connective tissue disease syndrome. Arch Dermatol. 1977 May;113(5):583–587. [PubMed] [Google Scholar]
  9. Gupta S., Good R. A. Subpopulations of human T lymphocytes. II. Effect of thymopoietin, corticosteroids, and irradiation. Cell Immunol. 1977 Nov;34(1):10–18. doi: 10.1016/0008-8749(77)90224-6. [DOI] [PubMed] [Google Scholar]
  10. Guyer R. L., Koshland M. E., Knopf P. M. Immunoglobulin binding by mouse intestinal epithelial cell receptors. J Immunol. 1976 Aug;117(2):587–593. [PubMed] [Google Scholar]
  11. Hopf U., Meyer zum Büschenfelde K. H., Dierich M. P. Demonstration of binding sites for IgG Fc and the third complement component (C3) on isolated hepatocytes. J Immunol. 1976 Aug;117(2):639–645. [PubMed] [Google Scholar]
  12. Liebeskind D. S., Hsu K. C., Erlanger B. F., Beiser S. M. Selective inhibition of transformed cells in culture by anti-thymidine antibodies. Nat New Biol. 1971 Nov 24;234(47):127–128. doi: 10.1038/newbio234127a0. [DOI] [PubMed] [Google Scholar]
  13. Messner R. P., Kennedy M. S., Jelinek J. G. Antilymphocyte antibodies in systemic lupus erythematosus. Effect on lymphocyte surface characteristics. Arthritis Rheum. 1975 May-Jun;18(3):201–206. doi: 10.1002/art.1780180302. [DOI] [PubMed] [Google Scholar]
  14. PLOTZ C. M., SINGER J. M. The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. Am J Med. 1956 Dec;21(6):888–892. [PubMed] [Google Scholar]
  15. Paronetto F., Koffler D. Immunofluorescent localization of immunoglobulins, complement, and fibrinogen in human diseases. I. Systemic lupus erythematosus. J Clin Invest. 1965 Oct;44(10):1657–1664. doi: 10.1172/JCI105272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. ROSENKRANZ H. S., ERLANGER B. F., TANENBAUM S. W., BEISER S. M. PURINE- AND PYRIMIDINE-SPECIFIC ANTIBODIES: EFFECT ON THE FERTILIZED SEA URCHIN EGG. Science. 1964 Jul 17;145(3629):282–283. doi: 10.1126/science.145.3629.282. [DOI] [PubMed] [Google Scholar]
  17. Rahman A. A., Teschner M., Sethi K. K., Brandis H. Appearance of IgG (Fc) receptor(s) on cultured human fibroblasts infected with human cytomegalovirus. J Immunol. 1976 Jul;117(1):253–258. [PubMed] [Google Scholar]
  18. Rivero S. J., Llorente L., Díaz-Jouanen E., Alarcón-Segovia D. T-lymphocyte subpopulation in untreated SLE. Variations with disease activity. Arthritis Rheum. 1977 Jul-Aug;20(6):1169–1173. doi: 10.1002/art.1780200602. [DOI] [PubMed] [Google Scholar]
  19. Samarut C., Brochier J., Revillard J. P. Distribution of cells binding erythrocyte-antibody (EA) complexes in human lymphoid populations. Scand J Immunol. 1976;5(3):221–231. doi: 10.1111/j.1365-3083.1976.tb00273.x. [DOI] [PubMed] [Google Scholar]
  20. Schimke R. N., Kirkpatrick C. H., Delp M. H. Calcinosis, Raynaud's phenomenon, sclerodactyly, and telangiectasia. The CRST syndrome. Arch Intern Med. 1967 Apr;119(4):365–370. [PubMed] [Google Scholar]
  21. Sharp G. C., Irvin W. S., LaRoque R. L., Velez C., Daly V., Kaiser A. D., Holman H. R. Association of autoantibodies to different nuclear antigens with clinical patterns of rheumatic disease and responsiveness to therapy. J Clin Invest. 1971 Feb;50(2):350–359. doi: 10.1172/JCI106502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sharp G. C., Irvin W. S., Tan E. M., Gould R. G., Holman H. R. Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA). Am J Med. 1972 Feb;52(2):148–159. doi: 10.1016/0002-9343(72)90064-2. [DOI] [PubMed] [Google Scholar]
  23. Stjernswärd J., Jondal M., Vánky F., Wigzell H., Sealy R. Lymphopenia and change in distribution of human B and T lymphocytes in peripheral blood induced by irradiation for mammary carcinoma. Lancet. 1972 Jun 24;1(7765):1352–1356. doi: 10.1016/s0140-6736(72)91091-4. [DOI] [PubMed] [Google Scholar]
  24. Svec K. H., Blair J. D., Kaplan M. H. Immunopathologic studies of systemic lupus erythematosus (SLE). I. Tissue-bound immunoglobulins in relation to serum antinuclear immunoglobulins in systemic lupus and in chronic liver disease with LE cell factor. J Clin Invest. 1967 Apr;46(4):558–568. doi: 10.1172/JCI105557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tan E. M., Kunkel H. G. An immunofluorescent study of the skin lesions in systemic lupus erythematosus. Arthritis Rheum. 1966 Feb;9(1):37–46. doi: 10.1002/art.1780090105. [DOI] [PubMed] [Google Scholar]
  26. Varesio L., Cappuccinelli P., Forni G. Inhibition of replication of virus-transformed fibroblasts by antibodies to RNA. Cancer Res. 1975 Dec;35(12):3558–3565. [PubMed] [Google Scholar]
  27. WATKINS J. F. ADSORPTION OF SENSITIZED SHEEP ERYTHROCYTES TO HELA CELLS INFECTED WITH HERPES SIMPLEX VIRUS. Nature. 1964 Jun 27;202:1364–1365. doi: 10.1038/2021364a0. [DOI] [PubMed] [Google Scholar]

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