Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1979 Jul;37(1):120–125.

In vitro testing of immunoresponsiveness in patients with inflammatory bowel disease: prevalence and relationship to disease activity immunoresponsiveness in IBD.

J J Lyanga, P Davis, A B Thomson
PMCID: PMC1537665  PMID: 314871

Abstract

Abnormalities in the numbers and function of thymus and function of thymus-derived and bone marrow-derived lymphocytes (T and B cells) and K cells were determined in sixty-nine consecutive patients with Crohn's disease or ulcerative colitis. Rosetting techniques to identify subpopulations of lymphocytes showed a significant decrease in E-rosettes (T cells) and significant increase in EA- and EAC-rosettes (B cells) in patients with inflammatory bowel disease when compared to normals. In vitro lymphocyte transformation responses to mitogens and antigens were depressed to a variable degree. Mean levels of K cell activity were not significantly different from normal controls. A considerable degree of individual variation was noted in all groups. When the results of each groups were considered, none of the laboratory variables correlated with the site, duration or activity of disease, therapy, presence of iron deficiency anemia, weight loss or hypoalbuminaemia. Thus, in vitro evidence of abnormal immune responses in patients with inflammatory bowel disease cannot be directly related to clinical or laboratory variables and probably reflects a multi-factorial aetiology.

Full text

PDF
123

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asquith P., Kraft S. C., Rothberg R. M. Lymphocyte responses to nonspecific mitogens in inflammatory bowel disease. Gastroenterology. 1973 Jul;65(1):1–7. [PubMed] [Google Scholar]
  2. Best W. R., Becktel J. M., Singleton J. W., Kern F., Jr Development of a Crohn's disease activity index. National Cooperative Crohn's Disease Study. Gastroenterology. 1976 Mar;70(3):439–444. [PubMed] [Google Scholar]
  3. Bolton P. M., James S. L., Newcombe R. G., Whitehead R. H., Hughes L. E. The immune competence of patients with inflammatory bowel disease. Gut. 1974 Mar;15(3):213–219. doi: 10.1136/gut.15.3.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandra R. K. Immunocompetence in undernutrition. J Pediatr. 1972 Dec;81(6):1194–1200. doi: 10.1016/s0022-3476(72)80262-2. [DOI] [PubMed] [Google Scholar]
  5. Foad B. S., Adams L. E., Yamauchi Y., Litwin A. Phytomitogen responses of peripheral blood lymphocytes in young and older subjects. Clin Exp Immunol. 1974 Aug;17(4):657–664. [PMC free article] [PubMed] [Google Scholar]
  6. Hagen C., Froland A. Analysis of the variation in lymphocyte response to PHA in normal subjects. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Apr;81(2):253–258. doi: 10.1111/j.1699-0463.1973.tb00219.x. [DOI] [PubMed] [Google Scholar]
  7. Joynson D. H., Walker D. M., Jacobs A., Dolby A. E. Defect of cell-mediated immunity in patients with iron-deficiency anaemia. Lancet. 1972 Nov 18;2(7786):1058–1059. doi: 10.1016/s0140-6736(72)92340-9. [DOI] [PubMed] [Google Scholar]
  8. Law D. K., Dudrick S. J., Abdou N. I. Immunocompetence of patients with protein-calorie malnutrition. The effects of nutritional repletion. Ann Intern Med. 1973 Oct;79(4):545–550. doi: 10.7326/0003-4819-79-4-545. [DOI] [PubMed] [Google Scholar]
  9. MacPherson B. R., Albertini R. J., Beeken W. L. Immunological studies in patients with Crohn's disease. Gut. 1976 Feb;17(2):100–106. doi: 10.1136/gut.17.2.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Meuwissen S. G., Schellekens P. T., Huismans L., Tytgat G. N. Impaired anamnestic cellular immune response in patients with Crohn's disease. Gut. 1975 Nov;16(11):854–860. doi: 10.1136/gut.16.11.854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meyers S., Sacher D. B., Taub R. N., Janowitz H. D. Anergy to dinitrochlorobenzene and depression of T-lymphocytes in Crohn's disease and ulcerative colitis. Gut. 1976 Nov;17(11):911–915. doi: 10.1136/gut.17.11.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Perlmann P., Perlmann H. Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes. Cell Immunol. 1970 Sep;1(3):300–315. doi: 10.1016/0008-8749(70)90051-1. [DOI] [PubMed] [Google Scholar]
  13. Russell A. S., Percy J. S., Kovithavongs T. Cell-mediated immunity to Herpes simplex in humans: lymphocyte cytotoxicity measured by 51-Cr release from infected cells. Infect Immun. 1975 Feb;11(2):355–359. doi: 10.1128/iai.11.2.355-359.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sachar D. B., Taub R. N., Brown S. M., Present D. H., Korelitz B. I., Janowitz H. D. Imparied lymphocyte responsiveness in inflammatory bowel disease. Gastroenterology. 1973 Feb;64(2):203–209. [PubMed] [Google Scholar]
  15. Skinner J. M., Whitehead R. A morphological assessment of immunoreactivity in colonic Crohn's disease and ulcerative colitis by a study of the lymph nodes. J Clin Pathol. 1974 Mar;27(3):202–206. doi: 10.1136/jcp.27.3.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Strickland R. G., Korsmeyer S., Soltis R. D., Wilson I. D., Williams R. C., Jr Peripheral blood T and B cells in chronic inflammatory bowel disease. Gastroenterology. 1974 Oct;67(4):569–577. [PubMed] [Google Scholar]
  17. Weiner M. S., Bianco C., Nussenzweig V. Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood. 1973 Dec;42(6):939–946. [PubMed] [Google Scholar]
  18. Whorwell P. J., Wright R. Immunological aspects of inflammatory bowel disease. Clin Gastroenterol. 1976 May;5(2):303–321. [PubMed] [Google Scholar]
  19. Zeylemaker W. P., Roos M. T., Meyer C. J., Schellekens P. T., Eijsvoogel V. P. Separation of human lymphocyte subpopulations. I. Transformation characteristics of rosette-forming cells. Cell Immunol. 1974 Dec;14(3):346–358. doi: 10.1016/0008-8749(74)90184-1. [DOI] [PubMed] [Google Scholar]
  20. Ziegler J. B., Hansen P. J., Davies W. A., Penny R. The PHA dose-response curve: validation of the use of logarithmic graph paper by computer analysis results. J Immunol. 1974 Dec;113(6):2035–2039. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES