Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1979 May;36(2):299–303.

Ontogeny of T cells, B cells and monocytes in the bovine foetus.

D R Senogles, P S Paul, D W Johnson, C C Muscoplat
PMCID: PMC1537733  PMID: 314369

Abstract

The ontogeny of lymphocyte subpopulations and monocytes was studied in developing bovine foetuses. Fifty-four bovine foetuses ranging in age from 3 to 9 months of gestation were collected and examined for the presence of thymus-derived (T lymphocytes), bone marrow-derived (B lymphocytes) and phagocytic (macrophage) cells from thymus, spleen and peripheral blood. Detection of the cells expressing T lymphocyte surface markers was done using a modified sheep red blood cell rosette assay. Lymphocytes bearing surface immunoglobulin (sIg) were detected by staining with a fluoresceinated rabbit antibody prepared against bovine IgG. The procedure used for detecting monocytes was a latex bead phagocytic assay. In the thymus, the percentage of T lymphocytes was relatively constant throughout gestation at approximately 60-70%, with B lymphocytes remaining at about 1%, whereas the phagocytic cells showed an increase from 1% at 3 months to approximately 8% at term. The spleen showed an increasing percentage of T lymphocytes, from 11% at 3 months to over 40% at term, whereas the percentage of B lymphocytes remained consistent at about 2-3% from 3 months until term. The percentage of monocytes increases from 1% at 3 months to over 20% at 9 months. Peripheral blood T lymphocytes increased steadily from approximately 1% at 3 months to 45% at term. B lymphocyte levels remained low, about 1%, throughout gestation, whereas monocytes increased from 1% at 3 months to 5% at 9 months.

Full text

PDF
301

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chapman H. A., Johnson J. S., Cooper M. D. Ontogeny of Peyer's patches and immunoglobulin-containing cells in pigs. J Immunol. 1974 Feb;112(2):555–563. [PubMed] [Google Scholar]
  2. Guez M., Sachs L. Purification of the protein that induces cell differentiation to macrophages and granulocytes. FEBS Lett. 1973 Dec 1;37(2):149–154. doi: 10.1016/0014-5793(73)80446-6. [DOI] [PubMed] [Google Scholar]
  3. Hardy B., Globerson A., Danon D. Ontogenic development of the reactivity of macrophages to antigenic stimulation. Cell Immunol. 1973 Nov;9(2):282–288. doi: 10.1016/0008-8749(73)90079-8. [DOI] [PubMed] [Google Scholar]
  4. Kearney J. F., Lawton A. R. B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes. J Immunol. 1975 Sep;115(3):671–676. [PubMed] [Google Scholar]
  5. Kearney J. F., Lawton A. R. B lymphocyte differentiation induced by lipopolysaccharide. II. Response of fetal lymphocytes. J Immunol. 1975 Sep;115(3):677–681. [PubMed] [Google Scholar]
  6. MARTINEZ C., KERSEY J., PAPERMASTER B. W., GOOD R. A. Skin homograft survival in thymectomized mice. Proc Soc Exp Biol Med. 1962 Jan;109:193–196. doi: 10.3181/00379727-109-27149. [DOI] [PubMed] [Google Scholar]
  7. Melchers F., Andersson J., Phillips R. A. Ontogeny of murine B lymphocytes: development of Ig synthesis and of reactivities to mitogens and to anti-Ig-antibodies. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):147–158. doi: 10.1101/sqb.1977.041.01.019. [DOI] [PubMed] [Google Scholar]
  8. Olson D. P., Waxler G. L. Immune responses of the bovine fetus and neonate to Escherichia coli: plaque-forming and intestinal immune responses. Am J Vet Res. 1977 Aug;38(8):1177–1181. [PubMed] [Google Scholar]
  9. Paul P. S., Pomeroy K. A., Johnson D. W., Muscoplat C. C., Handwerger B. S., Soper F. F., Sorensen D. K. Evidence for the replication of bovine leukemia virus in the B lymphocytes. Am J Vet Res. 1977 Jun;38(6):873–876. [PubMed] [Google Scholar]
  10. Paul P. S., Senogles D. R., Muscoplat C. C., Johnson D. W. Enumeration of T cells, B cells and monocytes in the peripheral blood of normal and lymphocytotic cattle. Clin Exp Immunol. 1979 Feb;35(2):306–316. [PMC free article] [PubMed] [Google Scholar]
  11. Senogles D. R., Muscoplat C. C., Paul P. S., Johnson D. W. Ontogeny of circulating B lymphocytes in neonatal calves. Res Vet Sci. 1978 Jul;25(1):34–36. [PubMed] [Google Scholar]
  12. Sidman C. L., Unanue E. R. Development of B lymphocytes. I. Cell populations and a critical event during ontogeny. J Immunol. 1975 Jun;114(6):1730–1735. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES