Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1979 Aug;37(2):359–366.

Antigens of the human trophoblast plasma membrane.

A Whyte, Y W Loke
PMCID: PMC1537819  PMID: 91464

Abstract

Using purified plasma membranes of human syncytial trophoblast as immunogen, we have raised heterologous antisera which reacted by indirect immunohistological methods with the surface of the trophoblast. This antigenic activity appears to be absent from other adult and foetal tissues. Its absence from maternal sera and its resistance to mild detergents treatment suggest that it is intimately associated with the surface membrane of the syncytial trophoblast. This antigen was found to be immunologically different to a number of proteins, including those known to be produced by the trophoblast. The identification of this antigen may indicate a potential line of research for the immunological regulation of human fertility.

Full text

PDF
366

Images in this article

362FIG. 2
on p.362
363FIG. 3
on p.363
364FIG. 4
on p.364

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allan D., Crumpton M. J. Preparation and characterization of the plasma membrane of pig lymphocytes. Biochem J. 1970 Nov;120(1):133–143. doi: 10.1042/bj1200133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avruch J., Wallach D. F. Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells. Biochim Biophys Acta. 1971 Apr 13;233(2):334–347. doi: 10.1016/0005-2736(71)90331-2. [DOI] [PubMed] [Google Scholar]
  3. Beer A. E., Billingham R. E., Yang S. L. Further evidence concerning the autoantigenic status of the trophoblast. J Exp Med. 1972 May 1;135(5):1177–1184. doi: 10.1084/jem.135.5.1177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Behrman S. J., Yoshida T., Amano Y., Paine P. Rhesus and squirrel monkey placental specific antigen(s). Am J Obstet Gynecol. 1974 Mar 1;118(5):616–625. doi: 10.1016/s0002-9378(16)33737-1. [DOI] [PubMed] [Google Scholar]
  5. Bradbury S., Billington W. D., Kirby D. R., Williams E. A. Histochemical characterization of the surface mucoprotein of normal and abnormal human trophoblast. Histochem J. 1970 Jul;2(4):263–274. doi: 10.1007/BF01004995. [DOI] [PubMed] [Google Scholar]
  6. Dimmette J. E. Studies of human pregnancy. I. Immunoglobulins attached to the trophoblast. Obstet Gynecol. 1976 Jun;47(6):730–732. [PubMed] [Google Scholar]
  7. Faulk W. P., Temple A. Distribution of beta2 microglobulin and HLA in chorionic villi of human placentae. Nature. 1976 Aug 26;262(5571):799–802. doi: 10.1038/262799a0. [DOI] [PubMed] [Google Scholar]
  8. Faulk W. P., Temple A., Lovins R. E., Smith N. Antigens of human trophoblasts: a working hypothesis for their role in normal and abnormal pregnancies. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1947–1951. doi: 10.1073/pnas.75.4.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GOLDBARG J. A., RUTENBURG A. M. The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases. Cancer. 1958 Mar-Apr;11(2):283–291. doi: 10.1002/1097-0142(195803/04)11:2<283::aid-cncr2820110209>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  10. Goodfellow P. N., Barnstable C. J., Bodmer W. F., Snary D., Crumpton M. J. Expression of HLA system antigens on placenta. Transplantation. 1976 Dec;22(6):595–603. doi: 10.1097/00007890-197612000-00009. [DOI] [PubMed] [Google Scholar]
  11. Goto S., Hoshino M., Tomoda Y., Ishizuka N. Innumoelectron microscopy of the human chorionic villus in search of blood group A and B antigens. Lab Invest. 1976 Dec;35(6):530–536. [PubMed] [Google Scholar]
  12. Johnson P. M., Faulk W. P. Immunological studies of human placentae: identification and distribution of proteins in immature chorionic villi. Immunology. 1978 Jun;34(6):1027–1035. [PMC free article] [PubMed] [Google Scholar]
  13. Loke Y. W., Ballard A. C. Blood group A antigens on human trophoblast cells. Nature. 1973 Oct 12;245(5424):329–330. doi: 10.1038/245329a0. [DOI] [PubMed] [Google Scholar]
  14. Loke Y. W., Joysey V. C., Borland R. HL-A antigens on human trophoblast cells. Nature. 1971 Aug 6;232(5310):403–405. doi: 10.1038/232403a0. [DOI] [PubMed] [Google Scholar]
  15. Ockleford C. D., Whyte A. Differeniated regions of human placental cell surface associated with exchange of materials between maternal and foetal blood: coated vesicles. J Cell Sci. 1977 Jun;25:293–312. doi: 10.1242/jcs.25.1.293. [DOI] [PubMed] [Google Scholar]
  16. Rigby P., Curzen P. Identification of human trophoblast in vitro. Nature. 1969 Jun 14;222(5198):1062–1063. doi: 10.1038/2221062b0. [DOI] [PubMed] [Google Scholar]
  17. Stevens V. C. Immunological approaches to fertility regulation. Bull World Health Organ. 1978;56(2):179–192. [PMC free article] [PubMed] [Google Scholar]
  18. Taylor C. R. An immunohistological study of follicular lymphoma, reticulum cell sarcoma and Hodgkin's disease. Eur J Cancer. 1976 Jan;12(1):61–75. doi: 10.1016/0014-2964(76)90125-0. [DOI] [PubMed] [Google Scholar]
  19. Whyte A. H.C.G. and trophoblast antigenicity. Lancet. 1978 Nov 4;2(8097):1003–1003. doi: 10.1016/s0140-6736(78)92578-3. [DOI] [PubMed] [Google Scholar]
  20. Whyte A., Loke Y. W. Increased sialylation of surface glycopeptides of human trophoblast compared with fetal cells from the same conceptus. J Exp Med. 1978 Oct 1;148(4):1087–1092. doi: 10.1084/jem.148.4.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Whyte A., Loke Y. W., Stoddart R. W. Saccharide distribution in human trophoblast demonstrated using fluorescein-labelled lectins. Histochem J. 1978 Jul;10(4):417–423. doi: 10.1007/BF01003005. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES