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. 1974 Oct;18(2):169–176.

Rosette formation by mouse lymphocytes. I. Demonstration by indirect immunofluorescence of T cells binding sheep erythrocytes

Maria I C Gyöngyössy, J H L Playfair
PMCID: PMC1537897  PMID: 4549624

Abstract

A method is described for indirect immunofluorescent staining of mouse T cells forming rosettes, involving fixation of rosettes by glutaraldehyde and staining with a rabbit anti-mouse brain serum absorbed so as to be specific for T cells. Using this technique, it was shown that about 44% of spleen cells forming rosettes with sheep erythrocytes both in non-immunized and immunized mice, are T cells. The number of T rosette-forming cells increased about ten-fold after immunization with sheep erythrocytes, but only when serum antibody was detectable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashman R. F., Raff M. C. Direct demonstration of theta-positive antigen-binding cells, with antigen-induced movement of thymus-dependent cell receptors. J Exp Med. 1973 Jan 1;137(1):69–84. doi: 10.1084/jem.137.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bach J. F., Muller J. Y., Dardenne M. In vivo specific antigen recognition by rosette forming cells. Nature. 1970 Sep 19;227(5264):1251–1252. doi: 10.1038/2271251a0. [DOI] [PubMed] [Google Scholar]
  3. Dennert G., Tucker D. F. Selective priming of T cells by chemically altered cell antigens. J Exp Med. 1972 Sep 1;136(3):656–661. doi: 10.1084/jem.136.3.656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Greaves M. F., Möller E., Möller G. Studies on antigen-binding cells. II. Relationship to antigen-sensitive cells. Cell Immunol. 1970 Oct;1(4):386–403. doi: 10.1016/0008-8749(70)90016-x. [DOI] [PubMed] [Google Scholar]
  5. Greaves M. F., Möller E. Studies on antigen-binding cells. I. The origin of reactive cells. Cell Immunol. 1970 Oct;1(4):372–385. doi: 10.1016/0008-8749(70)90015-8. [DOI] [PubMed] [Google Scholar]
  6. Gyöngyössy M. I., Playfair J. H. Indirect immunofluorescence of mouse thymus-derived cells using heterologous anti-brain serum. Cell Immunol. 1973 Apr;7(1):118–123. doi: 10.1016/0008-8749(73)90187-1. [DOI] [PubMed] [Google Scholar]
  7. Haskill J. S., Elliott B. E., Kerbel R., Axelrad M. A., Eidinger D. Classification of thymus-derived and marrow-derived lymphocytes by demonstration of their antigen-binding characteristics. J Exp Med. 1972 Jun 1;135(6):1410–1415. doi: 10.1084/jem.135.6.1410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Modabber F., Morikawa S., Coons A. H. Antigen-binding cells in normal mouse thymus. Science. 1970 Dec 4;170(3962):1102–1104. doi: 10.1126/science.170.3962.1102. [DOI] [PubMed] [Google Scholar]
  9. Playfair J. H. Strain differences in the immune response of mice. I. The neonatal response to sheep red cells. Immunology. 1968 Jul;15(1):35–50. [PMC free article] [PubMed] [Google Scholar]
  10. Playfair J. H. The role of antibody in T-cell responses. Clin Exp Immunol. 1974 May;17(1):1–18. [PMC free article] [PubMed] [Google Scholar]
  11. Raff M. C. Theta-bearing lymphocytes in nude mice. Nature. 1973 Dec 7;246(5432):350–351. doi: 10.1038/246350a0. [DOI] [PubMed] [Google Scholar]
  12. Raff M. Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969 Oct 25;224(5217):378–379. doi: 10.1038/224378a0. [DOI] [PubMed] [Google Scholar]
  13. Schlesinger M. Anti-theta antibodies for detecting thymus-dependent lymphocytes in the immune response of mice to SRBC. Nature. 1970 Jun 27;226(5252):1254–1256. doi: 10.1038/2261254a0. [DOI] [PubMed] [Google Scholar]
  14. Webb S. R., Cooper M. D. T cells can bind antigen via cytophilic IgM antibody made by B cells. J Immunol. 1973 Jul;111(1):275–277. [PubMed] [Google Scholar]
  15. Wilson J. D., Miller J. F. T and B rosette-forming cells. Eur J Immunol. 1971 Dec;1(6):501–503. doi: 10.1002/eji.1830010622. [DOI] [PubMed] [Google Scholar]

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