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. 1980 Jan;39(1):66–75.

Identification of cells expressing T and p28,33 (Ia-like) antigens in sections of human lymphoid tissue.

G J Seymour, M F Greaves, G Janossy
PMCID: PMC1537941  PMID: 6993067

Abstract

A method has been developed for identifying human lymphocyte populations in tissue sections using hetero-antisera to T cells and to the B cell associated Ia-like, p28,33 polypeptide complex. The differential topographical localisation of T and B cells is revealed by simultaneous staining with antibodies tagged with different fluorochromes; this approach also identifies a small population of T cells bearing Ia-like antigens. Preliminary studies on lymphomatous tissue suggests this approach will be of value in the study of malignancy and cellular infiltration in disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrahamsen T. G., Froland S. S., Natvig J. B., Pahle J. Elution and characterization of lymphocytes from rheumatoid inflammatory tissue. Scand J Immunol. 1975;4(8):823–830. doi: 10.1111/j.1365-3083.1975.tb03723.x. [DOI] [PubMed] [Google Scholar]
  2. Allison J. P., Walker L. E., Russell W. A., Pellegrino M. A., Ferrone S., Reisfeld R. A., Frelinger J. A., Silver J. Murine Ia and human DR antigens: homology of amino-terminal sequences. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3953–3956. doi: 10.1073/pnas.75.8.3953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Claudy A. L., Schmitt D., Viac J., Alario A., Staquet M. J., Thivolet J. Morphological, immunological and immunocytochemical identification of lymphocytes extracted from cutaneous infiltrates. Clin Exp Immunol. 1976 Jan;23(1):61–68. [PMC free article] [PubMed] [Google Scholar]
  4. Curran R. C., Jones E. L. Immunoglobulin-containing cells in human tonsils as demonstrated by immunohistochemistry. Clin Exp Immunol. 1977 Apr;28(1):103–115. [PMC free article] [PubMed] [Google Scholar]
  5. Davies A. J., Carter R. L., Leuchars E., Wallis V., Koller P. C. The morphology of immune reactions in normal, thymectomized and reconstituted mice. I. The response to sheep erythrocytes. Immunology. 1969 Jan;16(1):57–69. [PMC free article] [PubMed] [Google Scholar]
  6. Dukor P., Bianco C., Nussenzweig V. Tissue localization of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. Proc Natl Acad Sci U S A. 1970 Oct;67(2):991–997. doi: 10.1073/pnas.67.2.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  8. Giphart M. J., Kaufman J. F., Fuks A., Albrechtsen D., Solheim B. G., Bruning J. W., Strominger J. L. HLA-D associated alloantisera react with molecules similar to Ia antigens. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3533–3536. doi: 10.1073/pnas.74.8.3533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greaves M. F. Clinical applications of cell surface markers. Prog Hematol. 1975;9:255–303. [PubMed] [Google Scholar]
  10. Greaves M. F., Verbi W., Festenstein H., Papasteriadis C., Jaraquemada D., Hayward A. "Ia-like" antigens on human T cells. Eur J Immunol. 1979 May;9(5):356–362. doi: 10.1002/eji.1830090504. [DOI] [PubMed] [Google Scholar]
  11. Greenspan J. S., Gutman G. A., Talal N., Weissman I. L., Sugai S. Thymus-antigen- and immunoglobulin- positive cells in lymph-nodes, thymus, and malignant lymphomas of NZB/NZW mice. Clin Immunol Immunopathol. 1974 Sep;3(1):32–51. doi: 10.1016/0090-1229(74)90021-x. [DOI] [PubMed] [Google Scholar]
  12. Greenspan J. S., Gutman G. A., Weissman I. L., Talal N. Thymus-antigen- and immunoglobulin-positive lymphocytes in tissue infiltrates of NZB/NZW mice. Clin Immunol Immunopathol. 1974 Sep;3(1):16–31. doi: 10.1016/0090-1229(74)90020-8. [DOI] [PubMed] [Google Scholar]
  13. Gutman G. A., Weissman I. L. Lymphoid tissue architecture. Experimental analysis of the origin and distribution of T-cells and B-cells. Immunology. 1972 Oct;23(4):465–479. [PMC free article] [PubMed] [Google Scholar]
  14. Horwitz D. A., Niaudet P., Greaves M. F., Dorling J., Deteix P. Surface markers and electron microscopy of human blood L cells: a comparison with T and B lymphocytes. J Immunol. 1978 Aug;121(2):678–684. [PubMed] [Google Scholar]
  15. Howard J. C., Hunt S. V., Gowans J. L. Identification of marrow-derived and thymus-derived small lymphocytes in the lymphoid tissue and thoracic duct lymph of normal rats. J Exp Med. 1972 Feb 1;135(2):200–219. doi: 10.1084/jem.135.2.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Janossy G., Goldstone A. H., Capellaro D., Greaves M. F., Kulenkampff J., Pippard M., Welsh K. Differentiation linked expression of p28,33 (Ia-like) structures on human leukaemic cells. Br J Haematol. 1977 Nov;37(3):391–402. doi: 10.1111/j.1365-2141.1977.tb01010.x. [DOI] [PubMed] [Google Scholar]
  17. Lamelin J. P., Thomasset N., Andre C., Brochier J., Revillard J. P. Study of human T and B lymphocytes with heterologous antisera. III. Immunofluorescence studies on tonsil sections. Immunology. 1978 Sep;35(3):463–469. [PMC free article] [PubMed] [Google Scholar]
  18. Levine G. D., Polliack A. The T-cell nature of the lymphocytes in two human epithelial thymomas: a comparative immunologic, scanning and transmission electron microscopic study. Clin Immunol Immunopathol. 1975 Jul;4(2):199–208. doi: 10.1016/0090-1229(75)90055-0. [DOI] [PubMed] [Google Scholar]
  19. McMillan M., Cecka J. M., Murphy D. B., McDevitt H. O., Hood L. Structure of murine Ia antigens: partial NH2-terminal amino acid sequences of products of the I-E or I-C subregion. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5135–5139. doi: 10.1073/pnas.74.11.5135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mendes N. F., Miki S. S., Peixinho Z. F. Combined detection of human T and B lymphocytes by rosette formation with sheep erythrocytes and zymosan-C3 complexes. J Immunol. 1974 Aug;113(2):531–536. [PubMed] [Google Scholar]
  21. Messner R. P., Lindström F. D., Williams R. C., Jr Peripheral blood lymphocyte cell surface markers during the course of systemic lupus erythematosus. J Clin Invest. 1973 Dec;52(12):3046–3056. doi: 10.1172/JCI107503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Papamichail M., Sheldon P. J., Holborow E. J. T- and B-cell subpopulations in infectious mononucleosis. Clin Exp Immunol. 1974 Sep;18(1):1–11. [PMC free article] [PubMed] [Google Scholar]
  23. Parrott D. M., De Sousa M. Thymus-dependent and thymus-independent populations: origin, migratory patterns and lifespan. Clin Exp Immunol. 1971 May;8(5):663–684. [PMC free article] [PubMed] [Google Scholar]
  24. Parrott D. V., De Sousa M. A., East J. Thymus-dependent areas in the lymphoid organs of neonatally thymectomized mice. J Exp Med. 1966 Jan 1;123(1):191–204. doi: 10.1084/jem.123.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Scheid M., Boyse E. A., Carswell E. A., Old L. J. Serologically demonstrable alloantigens of mouse epidermal cells. J Exp Med. 1972 Apr 1;135(4):938–955. doi: 10.1084/jem.135.4.938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schlossman S. F., Chess L., Humphreys R. E., Strominger J. L. Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288–1292. doi: 10.1073/pnas.73.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Seymour G. J., Dockrell H. M., Greenspan J. S. Enzyme differentiation of lymphocyte subpopulations in sections of human lymph nodes, tonsils and periodontal disease. Clin Exp Immunol. 1978 Apr;32(1):169–178. [PMC free article] [PubMed] [Google Scholar]
  29. Seymour G. J., Dockrell H. M., Greenspan J. S. Fixation for immunofluorescence and enzyme histochemistry on serial sections. J Histochem Cytochem. 1976 Oct;24(10):1112–1115. doi: 10.1177/24.10.789760. [DOI] [PubMed] [Google Scholar]
  30. Snary D., Barnstable C. J., Bodmer W. F., Goodfellow P. N., Crumpton M. J. Cellular distrubtion, purification, and molecular nature of human Ia antigens. Scand J Immunol. 1977;6(5):439–452. doi: 10.1111/j.1365-3083.1977.tb02101.x. [DOI] [PubMed] [Google Scholar]
  31. Springer T. A., Kaufman J. F., Terhorst C., Strominger J. L. Purification and structural characterisation of human HLA-linked B-cell antigens. Nature. 1977 Jul 21;268(5617):213–218. doi: 10.1038/268213a0. [DOI] [PubMed] [Google Scholar]
  32. Stern P. L. Theta alloantigen on mouse and rat fibroblasts. Nat New Biol. 1973 Nov 21;246(151):76–78. doi: 10.1038/newbio246076a0. [DOI] [PubMed] [Google Scholar]
  33. Strom T. B., Tilney N. L., Carpenter C. B., Busch G. J. Identity and cytotoxic capacity of cells infiltrating renal allografts. N Engl J Med. 1975 Jun 12;292(24):1257–1263. doi: 10.1056/NEJM197506122922402. [DOI] [PubMed] [Google Scholar]
  34. Welsh K. I., Turner M. J. Preparation of antisera specific for human B cells by immunization of rabbits with immune complexes. Tissue Antigens. 1976 Sep;8(3):197–205. doi: 10.1111/j.1399-0039.1976.tb00569.x. [DOI] [PubMed] [Google Scholar]
  35. Winchester R. J., Ross G. D., Jarowski C. I., Wang C. Y., Halper J., Broxmeyer H. E. Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation. Proc Natl Acad Sci U S A. 1977 Sep;74(9):4012–4016. doi: 10.1073/pnas.74.9.4012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Winchester R. J., Wang C. Y., Halper J., Hoffman T. Studies with B-cell allo- and hetero-antisera: parallel reactivity and special properties. Scand J Immunol. 1976;5(6-7):745–757. doi: 10.1111/j.1365-3083.1976.tb03024.x. [DOI] [PubMed] [Google Scholar]
  37. Yata J., Klein G., Kobayashi N., Furukawa T., Yanagisawa M. Human thymus-lymphoid tissue antigen and its presence in leukaemia and lymphoma. Clin Exp Immunol. 1970 Dec;7(6):781–792. [PMC free article] [PubMed] [Google Scholar]

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