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. 1975 Jan;19(1):55–65.

Mixed lymphocyte culture stimulatory and responding capacity of lymphocytes from patients with lymphoproliferative diseases.

H Rühl, W Vogt, G Bochert, S Schmidt, R Moelle, H Schaoua
PMCID: PMC1538031  PMID: 128426

Abstract

Lymphocyte reactivity in vitro was studied in patients with Hodgkin's disease, chronic lymphocytic leukaemia and lymphosarcoma. The responding capacity to phytohaemagglutinin (PHA) was markedly depressed and delayed in all three groups of patients compared with the PHA response observed in lymphocyte cultures from normal individuals. In one-way mixed lymphocyte culture experiments a significant decrease in responding capacity of the patients' lymphocytes to lymphocytes from normal donors could be demonstrated. In contrast, the stimulatory capacity of the patients' lymphocytes was found to be intact, or only slightly reduced.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AISENBERG A. C. HODGKIN'S DISEASE--PROGNOSIS, TREATMENT AND ETIOLOGIC AND IMMUNOLOGIC CONSIDERATIONS. N Engl J Med. 1964 Mar 5;270:508–CONTD. doi: 10.1056/NEJM196403052701006. [DOI] [PubMed] [Google Scholar]
  2. AISENBERG A. C. Studies on delayed hypersensitivity in Hodgkin's disease. J Clin Invest. 1962 Nov;41:1964–1970. doi: 10.1172/JCI104654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown R. S., Haynes H. A., Foley H. T., Godwin H. A., Berard C. W., Carbone P. P. Hodgkin's disease. Immunologic, clinical, and histologic features of 50 untreated patients. Ann Intern Med. 1967 Aug;67(2):291–302. doi: 10.7326/0003-4819-67-2-291. [DOI] [PubMed] [Google Scholar]
  4. Chase M. W. Delayed-type hypersensitivity and the immunology of Hodgkin's disease, with a parallel examination of sarcoidosis. Cancer Res. 1966 Jun;26(6):1097–1120. [PubMed] [Google Scholar]
  5. Han T., Sokal J. E. Lymphocyte response to phytohemagglutinin in Hodgkin's disease. Am J Med. 1970 Jun;48(6):728–734. doi: 10.1016/s0002-9343(70)80008-0. [DOI] [PubMed] [Google Scholar]
  6. Hersh E. M., Oppenheim J. J. Impaired in vitro lymphocyte transformation in Hodgkin's disease. N Engl J Med. 1965 Nov 4;273(19):1006–1012. doi: 10.1056/NEJM196511042731903. [DOI] [PubMed] [Google Scholar]
  7. Jackson S. M., Garrett J. V., Craig A. W. Lymphocyte transformation changes during the clinical course of Hodgkin's disease. Cancer. 1970 Apr;25(4):843–850. doi: 10.1002/1097-0142(197004)25:4<843::aid-cncr2820250413>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  8. KELLY W. D., LAMB D. L., VARCO R. L., GOOD R. A. An investigation of Hodgkin's disease with respect to the problem of homotransplantation. Ann N Y Acad Sci. 1960 May 31;87:187–202. doi: 10.1111/j.1749-6632.1960.tb23192.x. [DOI] [PubMed] [Google Scholar]
  9. LAMB D., PILNEY F., KELLY W. D., GOOD R. A. A comparative study of the incidence of anergy in patients with carcinoma, leukemia, hodgkin's disease and other lymphomas. J Immunol. 1962 Oct;89:555–558. [PubMed] [Google Scholar]
  10. Lang J. M., Oberling F., Tongio M. M., Mayer S., Waitz R. Mixed-lymphocyte reaction as assay for immunological competence of lymphocytes from patients with hodgkin's disease. Lancet. 1972 Jun 10;1(7763):1261–1263. doi: 10.1016/s0140-6736(72)90982-8. [DOI] [PubMed] [Google Scholar]
  11. MILLER D. G., LIZARDO J. G., SNYDERMAN R. K. Homologous and heterologous skin transplantation in patients with lymphomatous disease. J Natl Cancer Inst. 1961 Mar;26:569–583. [PubMed] [Google Scholar]
  12. NOWELL P. C. Differentiation of human leukemic leukocytes in tissue culture. Exp Cell Res. 1960 Mar;19:267–277. doi: 10.1016/0014-4827(60)90007-0. [DOI] [PubMed] [Google Scholar]
  13. OPPENHEIM J. J., WHANG J., FREI E., 3rd IMMUNOLOGIC AND CYTOGENETIC STUDIES OF CHRONIC LYMPHOCYTIC LEUKEMIC CELLS. Blood. 1965 Aug;26:121–132. [PubMed] [Google Scholar]
  14. Papac R. J. Lymphocyte transformation in malignant lymphomas. Cancer. 1970 Aug;26(2):279–286. doi: 10.1002/1097-0142(197008)26:2<279::aid-cncr2820260206>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  15. Pentycross C. R. Lymphocyte transformation in diseases of lymphoid tissue. Proc R Soc Med. 1970 Apr;63(4):355–357. [PMC free article] [PubMed] [Google Scholar]
  16. Robbins J. H. Human peripheral blood in tissue culture and the action of phytohemagglutinin. Experientia. 1964 Mar 15;20(3):164–168. doi: 10.1007/BF02150714. [DOI] [PubMed] [Google Scholar]
  17. Robbins J. H., Levis W. R. Inherent inability of chronic lymphocytic leukaemia lymphocytes to respond to phytohaemagglutinin. Int Arch Allergy Appl Immunol. 1972;43(6):845–858. doi: 10.1159/000230902. [DOI] [PubMed] [Google Scholar]
  18. Rubin A. D., Havemann K., Dameshek W. Studies in chronic lymphocytic leukemia: further studies of the proliferative abnormality of the blood lymphocyte. Blood. 1969 Feb;33(2):313–328. [PubMed] [Google Scholar]
  19. Rühl H., Kirchner H., Bochert G. Kinetics of the Zn 2+ - stimulation of human peripheral lmphocytes in vitro. Proc Soc Exp Biol Med. 1971 Jul;137(3):1089–1092. [PubMed] [Google Scholar]
  20. Rühl H., Scholle H., Krichner H. Stimulation peripherer menschlicher Lymphozyten in vitro durch Zn IP bei patienten mit Lymphogranulomatose und chronischer lymphatischer Leukämie. Acta Haematol. 1971;46(6):326–337. doi: 10.1159/000208595. [DOI] [PubMed] [Google Scholar]
  21. Rühl H., Vogt W., Bochert G., Schmidt S., Schaoua H., Moelle R. Lymphocyte transformation and production of a human mononuclear leucocyte chemotactic factor in patients with Hodgkin's disease. Clin Exp Immunol. 1974 Jul;17(3):407–415. [PMC free article] [PubMed] [Google Scholar]
  22. SCHREK R., RABINOWITZ Y. Effects of phytohemagglutinin on rat and normal and leukemic human blood cells. Proc Soc Exp Biol Med. 1963 May;113:191–194. doi: 10.3181/00379727-113-28317. [DOI] [PubMed] [Google Scholar]
  23. Scamps R. A., Streeter A. M., O'Neill B. J. Immunoglobulin levels in chronic lymphocytic leukaemia. Med J Aust. 1971 Mar 6;1(10):535–536. doi: 10.5694/j.1326-5377.1971.tb87693.x. [DOI] [PubMed] [Google Scholar]
  24. Smith M. J., Browne E., Slungaard A. The impaired responsiveness of chronic lymphatic leukemia lymphocytes to allogeneic lymphocytes. Blood. 1973 Apr;41(4):505–509. [PubMed] [Google Scholar]
  25. Wilson J. D., Nossal G. J. Identification of human T and B lymphocytes in normal peripheral blood and in chronic lymphocytic leukaemia. Lancet. 1971 Oct 9;2(7728):788–791. doi: 10.1016/s0140-6736(71)92741-3. [DOI] [PubMed] [Google Scholar]

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