Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1980 Feb;39(2):477–485.

Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. I. A hybridoma secreting antibody against a marker specific for human B lymphocytes.

D A Brooks, I Beckman, J Bradley, P J McNamara, M E Thomas, H Zola
PMCID: PMC1538048  PMID: 6966995

Abstract

A hybridoma has been isolated from the products of fusion of a myeloma cell line with spleen cells from mice immunized with a human B cell line. After cloning, the hybridoma secretes antibody with the following properties: (i) Human B-lymphoblastoid cell lines react with the antibody while T and null cell lines do not. (ii) The antibody reacts with the majority of leucocytes in the blood of patients with CLL, but with a minority of cells in the blood of patients with AML or ALL of the null or T type. (iii) The antibody reacts with 9-21% of mononuclear cells in normal peripheral blood. The reacting cells are not T cells and overlap extensively with cells identified as B cells by other markers. The antigen identified by this antibody appears to be distinct from known B cell markers, and is put forward as a new B cell marker with diagnostic potential.

Full text

PDF
479

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billing R., Rafizadeh B., Drew I., Hartman G., Gale R., Terasaki P. Human B-lymphocyte antigens expressed by lymphocytic and myelocytic leukemia cells. I. Detection by rabbit antisera. J Exp Med. 1976 Jul 1;144(1):167–178. doi: 10.1084/jem.144.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brochier J., Abou-Hamed Y. A., Gueho J. P., Revillard J. P. Study of human T and B lymphocytes with heterologous antisera. I Preparation, specificity and properties of antisera. Immunology. 1976 Nov;31(5):749–758. [PMC free article] [PubMed] [Google Scholar]
  3. Brown G., Capellaro D., Greaves M. Leukemia-associated antigens in man. J Natl Cancer Inst. 1975 Dec;55(6):1281–1289. doi: 10.1093/jnci/55.6.1281. [DOI] [PubMed] [Google Scholar]
  4. Chess L., Levine H., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. VI. Further characterization of the surface Ig negative, E rosette negative (null cell) subset. J Immunol. 1975 Dec;115(6):1483–1487. [PubMed] [Google Scholar]
  5. Clements P. J., Levy J. Receptors for IgG (Fc receptors) on human lymphocytes: re-evaluation by multiple techniques. Clin Exp Immunol. 1978 Nov;34(2):281–287. [PMC free article] [PubMed] [Google Scholar]
  6. Cooper M. D., Keightley R. G., Wu L. Y., Lawton A. R., 3rd Developmental defects of T and B cell lines in humans. Transplant Rev. 1973;16:51–84. doi: 10.1111/j.1600-065x.1973.tb00117.x. [DOI] [PubMed] [Google Scholar]
  7. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Espinouse D., Touraine F., Schmitt D., Touraine J. L. Human B-lymphocyte heteroantigens: direct evidence for specificities not shared by monocytes. Clin Exp Immunol. 1978 Dec;34(3):379–387. [PMC free article] [PubMed] [Google Scholar]
  9. Forbes I. J., Zalewski P. D. A subpopulation of human B lymphocytes that rosette with mouse erythrocytes. Clin Exp Immunol. 1976 Oct;26(1):99–107. [PMC free article] [PubMed] [Google Scholar]
  10. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  11. Goodfellow P., Barnstable C., Jones E., Bodmer W. F., Crumpton M. J., Snary D. Production of specific antisera to human B lymphocytes. Tissue Antigens. 1976 Feb;7(2):105–117. doi: 10.1111/j.1399-0039.1976.tb01039.x. [DOI] [PubMed] [Google Scholar]
  12. Greaves M. F., Brown G. A human B lymphocyte specific antigen. Nat New Biol. 1973 Nov 28;246(152):116–119. doi: 10.1038/newbio246116a0. [DOI] [PubMed] [Google Scholar]
  13. Haegert D. G., Coombs R. R. Immunoglobulin-positive mononuclear cells in human peripheral blood: detection by mixed anti-globulin and direct anti-globulin-rosetting reactions. J Immunol. 1976 May;116(5):1426–1430. [PubMed] [Google Scholar]
  14. Hallberg T., Gurner B. W., Coombs R. R. Opsonic adherence of sensitized ox red cells to human lymphocytes as measured by rosette formation. Int Arch Allergy Appl Immunol. 1973;44(4):500–513. doi: 10.1159/000230956. [DOI] [PubMed] [Google Scholar]
  15. Horwitz D. A., Niaudet P., Greaves M. F., Dorling J., Deteix P. Surface markers and electron microscopy of human blood L cells: a comparison with T and B lymphocytes. J Immunol. 1978 Aug;121(2):678–684. [PubMed] [Google Scholar]
  16. Hämmerling G. J., Lemke H., Hämmerling U., Höhmann C., Wallich R., Rajewsky K. Monoclonal antibodies against murine cell surface antigens: anti-H-2, anti-Ia and anti-T cell antibodies. Curr Top Microbiol Immunol. 1978;81:100–106. doi: 10.1007/978-3-642-67448-8_15. [DOI] [PubMed] [Google Scholar]
  17. Ishii Y., Koshiba H., Ueno H., Maeyama I., Takami T. Characterization of human B lymphocyte specific antigens. J Immunol. 1975 Jan;114(1 Pt 2):466–469. [PubMed] [Google Scholar]
  18. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  19. Levy R., Dilley J., Lampson L. A. Human normal and leukemia cell surface antigens. Mouse monoclonal antibodies as probes. Curr Top Microbiol Immunol. 1978;81:164–169. doi: 10.1007/978-3-642-67448-8_26. [DOI] [PubMed] [Google Scholar]
  20. Mendes N. F., Miki S. S., Peixinho Z. F. Combined detection of human T and B lymphocytes by rosette formation with sheep erythrocytes and zymosan-C3 complexes. J Immunol. 1974 Aug;113(2):531–536. [PubMed] [Google Scholar]
  21. Moretta L., Ferrarini M., Durante M. L., Mingari M. C. Expression of a receptor for IgM by human T cells in vitro. Eur J Immunol. 1975 Aug;5(8):565–569. doi: 10.1002/eji.1830050812. [DOI] [PubMed] [Google Scholar]
  22. Preud'homme J. L., Labaume S. Immunofluorescent staining of human lymphocytes for the detection of surface immunoglobulins. Ann N Y Acad Sci. 1975 Jun 30;254:254–261. doi: 10.1111/j.1749-6632.1975.tb29175.x. [DOI] [PubMed] [Google Scholar]
  23. Schlossman S. F., Chess L., Humphreys R. E., Strominger J. L. Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288–1292. doi: 10.1073/pnas.73.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stathopoulos G., Elliott E. V. Formation of mouse or sheep red-blood-cell rosettes by lymphocytes from normal and leukaemic individuals. Lancet. 1974 Apr 6;1(7858):600–601. doi: 10.1016/s0140-6736(74)92655-5. [DOI] [PubMed] [Google Scholar]
  25. Sundqvist K. G., Wanger L. Influence of specificity of reagents and of behaviour of cell surface Ig on the detection and enumeration of immunoglobulin-bearing lymphocytes in humans. J Immunol Methods. 1978;21(3-4):229–236. doi: 10.1016/0022-1759(78)90149-7. [DOI] [PubMed] [Google Scholar]
  26. Trizio D., Cudkowicz G. Separation of T and B lymphocytes by nylon wool columns: evaluation of efficacy by functional assays in vivo. J Immunol. 1974 Oct;113(4):1093–1097. [PubMed] [Google Scholar]
  27. Welsh K. I., Turner M. J. Preparation of antisera specific for human B cells by immunization of rabbits with immune complexes. Tissue Antigens. 1976 Sep;8(3):197–205. doi: 10.1111/j.1399-0039.1976.tb00569.x. [DOI] [PubMed] [Google Scholar]
  28. Zola H. Fractionation of human lymphocytes using rosette formation with papain-treated mouse erythrocytes. J Immunol Methods. 1977;18(3-4):387–389. doi: 10.1016/0022-1759(77)90193-4. [DOI] [PubMed] [Google Scholar]
  29. Zola H. Preparation of antisera with specificity for human T lymphocytes. Transplantation. 1977 Mar;23(3):222–229. doi: 10.1097/00007890-197703000-00004. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES