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. 1975 May;20(2):209–216.

Characterization of a new antigen-antibody system associated with hepatitis B.

L O Magnius
PMCID: PMC1538195  PMID: 55324

Abstract

The occurrence of a new antigen-antibody system was recently described in hepatitis B surface antigen (HBsAg) positive sera with different specificities, one of which was designated e. These specificities are related as demonstrated by the presently utilized e reagents not discriminating between them in immunodiffusion tests. Hence, they are collectively referred to as e in this report. The occurrence of e antibodies in sera containing antibodies against hepatitis B surface antigen (anti-HBs) is reported. The e antibodies were found to move as 7S globulin on rate zonal centrifugation. The e precipitate was demonstrated on the cathode side of the HBsAg precipitate on immunoelectrophoresis consistent with e antigen migrating in the gamma-globulin region. The e antigen is reported to have an S value of 11-6 +/- 0-6 (s.d.) and a buoyant density of 1-291 +/- 0-003 (s.d.), hence differing from HBsAg in all respects studied thus far.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bancroft W. H., Mundon F. K., Russell P. K. Detection of additional antigenic determinants of hepatitis B antigen. J Immunol. 1972 Oct;109(4):842–848. [PubMed] [Google Scholar]
  2. CHARLWOOD P. A. Applications of radioactively labeled marker proteins in density gradient ultracentrifugation. Anal Biochem. 1963 Mar;5:226–245. doi: 10.1016/0003-2697(63)90120-9. [DOI] [PubMed] [Google Scholar]
  3. GRABAR P., URIEL J., COURCON J. [Immuno-electrophoretic analysis of normal human serum. IV. Electrophoretic mobilities]. Ann Inst Pasteur (Paris) 1960 Jul;99:13–27. [PubMed] [Google Scholar]
  4. Grob P. J., Jemelka H. I. Fecal SH-antigen in acute hepatitis. Am J Dis Child. 1972 Apr;123(4):400–401. doi: 10.1001/archpedi.1972.02110100132048. [DOI] [PubMed] [Google Scholar]
  5. Iwarson S., Magnius L., Lindholm A., Lundin P. Subtypes of Hepatitis B Antigen in Blood Donors and Post-transfusion Hepatitis: Clinical and Epidemiological Aspects. Br Med J. 1973 Jan 13;1(5845):84–87. doi: 10.1136/bmj.1.5845.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jeansson S. Differentiation between herpes simplex virus type 1 and type 2 strains by immunoelectroosmophoresis. Appl Microbiol. 1972 Jul;24(1):96–100. doi: 10.1128/am.24.1.96-100.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kim C. Y., Tilles J. G. Immunologic and electrophoretic heterogeneity of hepatitis-associated antigen. J Infect Dis. 1971 Jun;123(6):618–628. doi: 10.1093/infdis/123.6.618. [DOI] [PubMed] [Google Scholar]
  8. Le Bouvier G. L. The heterogeneity of Australia antigen. J Infect Dis. 1971 Jun;123(6):671–675. doi: 10.1093/infdis/123.6.671. [DOI] [PubMed] [Google Scholar]
  9. Levene C., Blumberg B. S. Additional specificities of Australia antigen and the possible identification of hepatitis carriers. Nature. 1969 Jan 11;221(5176):195–196. doi: 10.1038/221195a0. [DOI] [PubMed] [Google Scholar]
  10. Magnius L. O., Espmark J. A. New specificities in Australia antigen positive sera distinct from the Le Bouvier determinants. J Immunol. 1972 Nov;109(5):1017–1021. [PubMed] [Google Scholar]
  11. Magnius L. O., Lindholm A., Lundin P., Iwarson S. A new antigen-antibody system. Clinical significance in long-term carriers of hepatitis B surface antigen. JAMA. 1975 Jan 27;231(4):356–359. doi: 10.1001/jama.231.4.356. [DOI] [PubMed] [Google Scholar]
  12. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  13. Raunio V. K., London W. T., Sutnick A. I., Millman I., Blumberg B. S. Specificities of human antibodies to Australia antigen. Proc Soc Exp Biol Med. 1970 Jun;134(2):548–557. doi: 10.3181/00379727-134-34833. [DOI] [PubMed] [Google Scholar]

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