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Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1975 Aug;21(2):216–225.

Observations on the antibody-dependent cytotoxic cell by scanning electron microscopy.

J R Inglis, W J Penhale, A Farmer, W J Irvine, A E Williams
PMCID: PMC1538261  PMID: 1191386

Abstract

The cytotoxic effect of human peripheral blood leucocytes on antibody-coated sheep erythrocyte monolayers has been investigated using scanning electron microscopy. Only a small proportion of leucocytes were found to adhere to the monolayers. A progressive destruction was observed beginning as small plaque-like areas of erythrocyte clearing which later became confluent. Three distinct cell types were found to be associated with the areas of lysis. No destruction was observed in control monolayers incubated for a similar period in the absence of either antibody of leucocytes. Surface changes in the erthrocytes adjacent to the leucocytes suggest that mechanical factors may be involved in erythrocyte lysis in this system. It is concluded that more than one leucocyte type may damage antibody-coated erythrocytes, possibly by a mechanism involving attachment to and mechanical disruption of the red cell membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calder E. A., Urbaniak S. J., Penhale W. J., Irvine W. J. Characterization of human lymphoid cell-mediated antibody-dependent cytotoxicity (LDAC). Clin Exp Immunol. 1974 Dec;18(4):579–593. [PMC free article] [PubMed] [Google Scholar]
  2. Carr I., Clarke J. A., Salsbury A. J. The surface structure of mouse peritoneal cells--a study with the scanning electron microscope. J Microsc. 1969;89(1):105–111. doi: 10.1111/j.1365-2818.1969.tb00654.x. [DOI] [PubMed] [Google Scholar]
  3. Carr K., Carr I. How cells settle on glass: a study by light and scanning electron microscopy of some properties of normal and stimulated macrophages. Z Zellforsch Mikrosk Anat. 1970;105(2):234–241. doi: 10.1007/BF00335473. [DOI] [PubMed] [Google Scholar]
  4. Cerottini J. C., Brunner K. T. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974;18:67–132. doi: 10.1016/s0065-2776(08)60308-9. [DOI] [PubMed] [Google Scholar]
  5. Dennert G., Lennox E. S. Phagocytic cells as effectors in a cell-mediated immunity system. J Immunol. 1973 Dec;111(6):1844–1854. [PubMed] [Google Scholar]
  6. Fagraeus A., Lidman K., Biberfeld G. Reaction of human smooth muscle antibodies with human blood lymphocytes and lymphoid cell lines. Nature. 1974 Nov 15;252(5480):246–247. doi: 10.1038/252246a0. [DOI] [PubMed] [Google Scholar]
  7. Forman J., Möller G. The effector cell in antibody-induced cell mediated immunity. Transplant Rev. 1973;17(0):108–149. doi: 10.1111/j.1600-065x.1973.tb00125.x. [DOI] [PubMed] [Google Scholar]
  8. Greenberg A. H., Shen L., Roitt I. M. Characterization of the antibody-dependent cytotoxic cell. A non-phagocytic monocyte? Clin Exp Immunol. 1973 Oct;15(2):251–259. [PMC free article] [PubMed] [Google Scholar]
  9. Kennedy J. C., Axelrad M. A. An improved assay for haemolytic plaque-forming cells. Immunology. 1971 Feb;20(2):253–257. [PMC free article] [PubMed] [Google Scholar]
  10. Linthicum D. S., Sell S., Wagner R. M., Trefts P. Scanning immunoelectron microscopy of mouse B and T lymphocytes. Nature. 1974 Nov 8;252(5479):173–175. doi: 10.1038/252173a0. [DOI] [PubMed] [Google Scholar]
  11. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  12. Möller G., Svehag S. E. Specificiy of lymphocyte-mediated cytotoxicity induced by in vitro antibody-coated target cells. Cell Immunol. 1972 May;4(1):1–19. doi: 10.1016/0008-8749(72)90001-9. [DOI] [PubMed] [Google Scholar]
  13. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  14. Perlmann P., Perlmann H., Wigzell H. Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells. Transplant Rev. 1972;13:91–114. doi: 10.1111/j.1600-065x.1972.tb00061.x. [DOI] [PubMed] [Google Scholar]
  15. Perper R. J., Zee T. W., Mickelson M. M. Purification of lymphocytes and platelets by gradient centrifugation. J Lab Clin Med. 1968 Nov;72(5):842–848. [PubMed] [Google Scholar]
  16. Polliack A., Lampen N., Clarkson B. D., De Harven E., Bentwich Z., Siegal F. P., Kunkel H. G. Identification of human B and T lymphocytes by scanning electron microscopy. J Exp Med. 1973 Sep 1;138(3):607–624. doi: 10.1084/jem.138.3.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ramsey W. S. Locomotion of human polymorphonuclear leucocytes. Exp Cell Res. 1972 Jun;72(2):489–501. doi: 10.1016/0014-4827(72)90019-5. [DOI] [PubMed] [Google Scholar]
  18. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]

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