Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1975 Nov;22(2):302–310.

Spontaneous autorosette-forming cells in man. A marker for a subset population of T lymphocytes?

J C Gluckman, P Montambault
PMCID: PMC1538300  PMID: 1082402

Abstract

A subpopulation of human peripheral blood lymphocytes are capable of binding with human (autologous or allogeneic) erythrocytes, forming rosettes. The conditions which lead to autorosette formation are similar to those required for sheep red-cell rosetting. Ageing human erythrocytes are shown to bear less of the determinants involved in the phenomenon than younger ones. Evidence is presented that autorosetting is a T-cell marker. As autorosette-forming cells are very sensitive to the inhibiting effects of ATG they could therefore belong to a T-cell subpopulation.

Full text

PDF
308

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Denman A. M., Barnes R. D. Cooperating and controlling functions of thymus-derived lymphocytes in relation to autoimmunity. Lancet. 1971 Jul 17;2(7716):135–140. doi: 10.1016/s0140-6736(71)92306-3. [DOI] [PubMed] [Google Scholar]
  2. Bach J. F., Judet C., Arce S., Dormont J. Exploration de la fonction thymique chez l'homme. II. Le phénomène des "rosettes-mouton", marqueur des lymphocytes T chez l'homme. Nouv Presse Med. 1974 Mar 16;3(11):655–660. [PubMed] [Google Scholar]
  3. Baxley G., Bishop G. B., Cooper A. G., Wortis H. H. Rosetting of human red blood cells to thymocytes and thymus-derived cells. Clin Exp Immunol. 1973 Nov;15(3):385–392. [PMC free article] [PubMed] [Google Scholar]
  4. Brown G., Greaves M. F. Cell surface markers for human T and B lymphocytes. Eur J Immunol. 1974 Apr;4(4):302–310. doi: 10.1002/eji.1830040414. [DOI] [PubMed] [Google Scholar]
  5. Charreire J., Bach J. F. Letter: Self and not-self. Lancet. 1974 Aug 3;2(7875):299–300. doi: 10.1016/s0140-6736(74)91474-3. [DOI] [PubMed] [Google Scholar]
  6. Coombs R. R., Gurner B. W., Wilson A. B., Holm G., Lindgren B. Rosette-formation between human lymphocytes and sheep red cells not involving immunoglobulin receptors. Int Arch Allergy Appl Immunol. 1970;39(5-6):658–663. doi: 10.1159/000230390. [DOI] [PubMed] [Google Scholar]
  7. Dewar A. E., Stuart A. E., Parker A. C., Wilson C. Letter: Rosetting cells in autoimmune haemolytic anaemia. Lancet. 1974 Aug 31;2(7879):519–520. doi: 10.1016/s0140-6736(74)92041-8. [DOI] [PubMed] [Google Scholar]
  8. Galili U., Schlesinger M. The formation of stable E rosettes after neuraminidase treatment of either human peripheral blood lymphocytes or of sheep red blood cells. J Immunol. 1974 May;112(5):1628–1634. [PubMed] [Google Scholar]
  9. Gattegno L., Bladier D., Cornillot P. Ageing in vivo and neuraminidase treatment of rabbit erythrocytes: influence on half-life as assessed by 51Cr labelling. Hoppe Seylers Z Physiol Chem. 1975 Apr;356(4):391–397. doi: 10.1515/bchm2.1975.356.1.391. [DOI] [PubMed] [Google Scholar]
  10. Gluckman J. C., Gattegno L., Cornillot P. Significance of spontaneous autorosettes in rats. Eur J Immunol. 1975 May;5(5):301–306. doi: 10.1002/eji.1830050503. [DOI] [PubMed] [Google Scholar]
  11. Gluckman J. C., Montambault P. Letter: Spontaneous autorosettes in man. Lancet. 1975 Jan 11;1(7898):112–113. doi: 10.1016/s0140-6736(75)91130-7. [DOI] [PubMed] [Google Scholar]
  12. Greenwalt T. J., Flory L. L., Steane E. A. Quantitative haemagglutination. 3. Stuies of separated populations of human red blood cells of different densities. Br J Haematol. 1970 Dec;19(6):701–709. doi: 10.1111/j.1365-2141.1970.tb07015.x. [DOI] [PubMed] [Google Scholar]
  13. Greenwalt T. J., Steane E. A. Quantitative haemagglutination. 4. Effect of neuraminidase treatment on agglutination by blood group antibodies. Br J Haematol. 1973 Aug;25(2):207–215. doi: 10.1111/j.1365-2141.1973.tb01732.x. [DOI] [PubMed] [Google Scholar]
  14. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kaplan J. Human T lymphocytes form rosettes with autologous and allogeneic human red blood cells. Clin Immunol Immunopathol. 1975 Mar;3(4):471–475. doi: 10.1016/0090-1229(75)90071-9. [DOI] [PubMed] [Google Scholar]
  16. Lay W. H., Mendes N. F., Bianco C., Nussenzweig V. Binding of sheep red blood cells to a large population of human lymphocytes. Nature. 1971 Apr 23;230(5295):531–532. doi: 10.1038/230531a0. [DOI] [PubMed] [Google Scholar]
  17. Lohrmann Hans-Peter, Novikovs L. Rosette formation between human T-lymphocytes and unsensitized rhesus monkey erythrocytes. Clin Immunol Immunopathol. 1974 Sep;3(1):99–111. doi: 10.1016/0090-1229(74)90027-0. [DOI] [PubMed] [Google Scholar]
  18. Micklem H. S., Asfi C., Staines N. A., Anderson N. Quantitative study of cells reacting to skin allografts. Nature. 1970 Aug 29;227(5261):947–949. doi: 10.1038/227947a0. [DOI] [PubMed] [Google Scholar]
  19. O'CONNELL D. J., CARUSO C. J., SASS M. D. SEPARATION OF ERYTHROCYTES OF DIFFERENT AGES. Clin Chem. 1965 Aug;11:771–781. [PubMed] [Google Scholar]
  20. Owen F. L., Fanger M. W. Studies on the human T-lymphocyte population. I. The development and characterization of a specific anti-human T-cell antibody. J Immunol. 1974 Oct;113(4):1128–1137. [PubMed] [Google Scholar]
  21. Owen F. L., Fanger M. W. Studies on the human T-lymphocyte population. II. The use of a T-cell specific antibody in the partial isolation and characterization of the human lymphocyte receptor for sheep red blood cells. J Immunol. 1974 Oct;113(4):1138–1144. [PubMed] [Google Scholar]
  22. PRENTICE T. C., BISHOP C. SEPARATION OF RABBIT RED CELLS BY DENSITY METHODS AND CHARACTERISTICS OF SEPARATED LAYERS. J Cell Physiol. 1965 Feb;65:113–125. doi: 10.1002/jcp.1030650114. [DOI] [PubMed] [Google Scholar]
  23. Pang G. T., Baguley D. M., Wilson J. D. Spontaneous rosettes as a T-lymphocyte marker: a modified method giving consistent results. SRBC rosettes. J Immunol Methods. 1974 Jan;3(1):41–46. doi: 10.1016/0022-1759(74)90031-3. [DOI] [PubMed] [Google Scholar]
  24. Sandilands G., Gray K., Cooney A., Browning J. D., Anderson J. R. Letter: Auto-rosette formation by human thymocytes and lymphocytes. Lancet. 1974 Jan 5;1(7845):27–28. doi: 10.1016/s0140-6736(74)93019-0. [DOI] [PubMed] [Google Scholar]
  25. Siegel I., Sherman W. B. The interaction of lymphocytes with autologous red cells. J Allergy Clin Immunol. 1972 Aug;50(2):65–74. doi: 10.1016/0091-6749(72)90001-2. [DOI] [PubMed] [Google Scholar]
  26. Whittingham S., Mackay I. R. Rosette formation by human thymocytes. Cell Immunol. 1973 Mar;6(3):362–367. doi: 10.1016/0008-8749(73)90036-1. [DOI] [PubMed] [Google Scholar]
  27. Worth H. H., Cooper A. G., Brown M. C. Inhibition of human lymphocyte rosetting by anti-T sera. Nat New Biol. 1973 May 23;243(125):109–111. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES