Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1976 May;24(2):300–309.

Ultrastructure of cells infiltrating human kidney allografts.

B Nabarra, B Descamps
PMCID: PMC1538422  PMID: 776465

Abstract

Cell infiltration is commonly observed in human renal allograft biopsies. This infiltration was investigated using electron microscopy for a more precise assessment of the nature of these cells. More than 3000 cells infiltrating twenty-five renal allograft biopsies were studied. Six cellular types were distinguished and a mean percentage of each type was calculated. Only one-half of these cells were normal or transformed lymphocytes (including small lymphocytes: 22-3 +/- 3-8%, 'intermediary' cells: 22 +/- 3-6%, blast-like cells similar to MLC transformed lymphocytes: 8-1 +/- 2-4%. A relatively high number of plasmocytes (12-4 +/- 2-5%) and a still higher percentage of macrophages (28-5 +/- 4-6%) were found. Granulocytes represented only 2 +/- 0-8%of the cell population. Variations of the mean percentage of these cellular types were studied in various clinical situations.

Full text

PDF
303

Images in this article

301FIG. 1-2
on p.301
302FIG. 3
on p.302
303FIG. 4
on p.303
304FIG. 5
on p.304
304FIG. 6
on p.304
305FIG. 7
on p.305
306FIG. 8
on p.306
308FIG. 9
on p.308

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander E. L., Wetzel B. Human lymphocytes: similarity of B and T cell surface morphology. Science. 1975 May 16;188(4189):732–734. doi: 10.1126/science.1079095. [DOI] [PubMed] [Google Scholar]
  2. BAIN B., VAS M. R., LOWENSTEIN L. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES. Blood. 1964 Jan;23:108–116. [PubMed] [Google Scholar]
  3. DEMPSTER W. J., WILLIAMS M. A. Cellular infiltration in homotransplanted kidneys. Br Med J. 1963 Jan 5;1(5322):18–23. [PMC free article] [PubMed] [Google Scholar]
  4. Dimitriu A., Dy M., Thomson N., Hamburger J. Macrophage cytotoxicity in the mouse immune response against a skin allograft. J Immunol. 1975 Jan;114(1 Pt 1):195–199. [PubMed] [Google Scholar]
  5. Feldman J. D., Lee S. Renal homotransplantation in rats. I. Allogeneic recipients. J Exp Med. 1967 Nov 1;126(5):783–794. doi: 10.1084/jem.126.5.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guttmann R. D., Lindquist R. R., Parker R. M., Carpenter C. B., Merrill J. P. Renal transplantation in the inbred rat. I. Morphologic, immunologic, and functional alterations during acute rejection. Transplantation. 1967 Jul;5(4):668–681. doi: 10.1097/00007890-196707000-00010. [DOI] [PubMed] [Google Scholar]
  7. HAMBURGER J., CROSNIER J., DORMONT J. EXPERIENCE WITH 45 RENAL HOMOTRANSPLANTATIONS IN MAN. Lancet. 1965 May 8;1(7393):985–992. doi: 10.1016/s0140-6736(65)91218-3. [DOI] [PubMed] [Google Scholar]
  8. Hall J. G., Morris B., Moreno G. D., Bessis M. C. The ultrastructure and function of the cells in lymph following antigenic stimulation. J Exp Med. 1967 Jan 1;125(1):91–110. doi: 10.1084/jem.125.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hamburger J., Dimitriu A., Bankir L., Debray-Sachs M., Auvert J. Collection of lymph from kidneys homotransplanted in man: cell transformation in vivo. Nature. 1971 Aug 27;232(5313):633–634. doi: 10.1038/232633a0. [DOI] [PubMed] [Google Scholar]
  10. Harris T. N., Hummeler K., Harris S. Electron microscopic observations on antibody-producing lymph node cells. J Exp Med. 1966 Jan 1;123(1):161–172. doi: 10.1084/jem.123.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lindquist R. R., Guttmann R. D., Merrill J. P., Dammin G. J. Human renal allografts. Interpretation of morphologic and immunohistochemical observations. Am J Pathol. 1968 Nov;53(5):851–881. [PMC free article] [PubMed] [Google Scholar]
  12. Lindquist R. R., Guttmann R. D., Merrill J. P. Renal transplantation in the inbred rat. VI. Electron microscopic study of the mononuclear cells accumulating in rejecting renal allografts. Transplantation. 1971 Jul;12(1):1–10. [PubMed] [Google Scholar]
  13. Matter A., Lisowska-Bernstein B., Ryser J. E., Lamelin J. P., Vassalli P. Mouse thymus-independent and thymus-derived lymphoid cells. II. Ultrastructural studies. J Exp Med. 1972 Nov 1;136(5):1008–1030. doi: 10.1084/jem.136.5.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PORTER K. A. MORPHOLOGICAL ASPECTS OF RENAL HOMOGRAFT REJECTION. Br Med Bull. 1965 May;21:171–175. doi: 10.1093/oxfordjournals.bmb.a070388. [DOI] [PubMed] [Google Scholar]
  15. Pedersen N. C., Morris B. The role of the lymphatic system in the rejection of homografts: a study of lymph from renal transplants. J Exp Med. 1970 May 1;131(5):936–969. doi: 10.1084/jem.131.5.936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Polliack A., Lampen N., Clarkson B. D., De Harven E., Bentwich Z., Siegal F. P., Kunkel H. G. Identification of human B and T lymphocytes by scanning electron microscopy. J Exp Med. 1973 Sep 1;138(3):607–624. doi: 10.1084/jem.138.3.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. REBUCK J. W., LOGRIPPO G. A. Characteristics and interrelationships of the various cells in the RE cell, macrophage, lymphocyte, and plasma cell series in man. Lab Invest. 1961 Nov-Dec;10:1068–1093. [PubMed] [Google Scholar]
  18. Rosenau W., Lee J. C., Najarian J. S. A light, fluorescence, and electron microscopic study of functioning human renal transplants. Surg Gynecol Obstet. 1969 Jan;128(1):62–76. [PubMed] [Google Scholar]
  19. Rowlands D. T., Jr, Burkholder P. M., Bossen E. H., Lin H. H. Renal allografts in HL-A matched recipients. Light, immunofluorescence and electron microscopic studies. Am J Pathol. 1970 Nov;61(2):177–210. [PMC free article] [PubMed] [Google Scholar]
  20. Strom T. B., Tilney N. L., Carpenter C. B., Busch G. J. Identity and cytotoxic capacity of cells infiltrating renal allografts. N Engl J Med. 1975 Jun 12;292(24):1257–1263. doi: 10.1056/NEJM197506122922402. [DOI] [PubMed] [Google Scholar]
  21. TANAKA Y., EPSTEIN L. B., BRECHER G., STOHLMAN F., Jr TRANSFORMATION OF LYMPHOCYTES IN CULTURES OF HUMAN PERIPHERAL BLOOD. Blood. 1963 Nov;22:614–629. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES