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. 1975 Dec;22(3):493–501.

Formation of auto-rosettes by peripheral blood lymphocytes.

G P Sandilands, K Gray, A Cooney, J D Browning
PMCID: PMC1538448  PMID: 1083787

Abstract

A mean of 3-4% (0-5-19-5%) of the peripheral blood lymphocytes of normal adults were shown to bind three or more autologous erythrocytes in vitro to form auto-rosettes. Marked individual fluctuations were observed. Increased percentages were observed in patients with cancer, but not in other selected groups, including a group who had undergone thymectomy for myasthenia gravis. Auto-rosette formation was shown to be a property of T cells by the demonstration of (a) simultaneous binding of autologous and sheep erythrocytes, (b) non-inhibition of auto-rosette formation by anti-immunoglobulin, and (c) formation of auto-rosettes by mitogen-stimulated T blasts. Auto rosette formation is a property of high percentages of human thymocytes, and of lymphocytes treated with neuraminidase or stimulated to blast-cell trans-formation by phytomitogens. It is suggested that auto-rosette formation by these cells is related to their relatively low content of cell-coat sialic acid, as compared with untreated T lymphocytes. The possible influence of the cell coat on lymphocyte function is discussed briefly.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anteunis A. Cytochemical and ultrastructural studies concerning the cell coat glycoproteins in normal and transformed human blood lymphocytes. I. Variations of sialic acid containing glycoproteins subsequent to transformation of T and B lymphocytes by various kinds of stimulating agents. Exp Cell Res. 1974 Mar 15;84(1):31–39. doi: 10.1016/0014-4827(74)90376-0. [DOI] [PubMed] [Google Scholar]
  2. Baxley G., Bishop G. B., Cooper A. G., Wortis H. H. Rosetting of human red blood cells to thymocytes and thymus-derived cells. Clin Exp Immunol. 1973 Nov;15(3):385–392. [PMC free article] [PubMed] [Google Scholar]
  3. Bentwich Z., Douglas S. D., Skutelsky E., Kunkel H. G. Sheep red cell binding to human lymphocytes treated with neuraminidase; enhancement of T cell binding and identification of a subpopulation of B cells. J Exp Med. 1973 Jun 1;137(6):1532–1537. doi: 10.1084/jem.137.6.1532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bona C., Anteunis A. Structure of the lymphocyte membrane. IV. Cell coat of lymphocytes obtained from various lymphoid organs in chicken and mice. Ann Immunol (Paris) 1973 Aug;124(3):321–344. [PubMed] [Google Scholar]
  6. Brain P., Marston R. H. Rosette formation by human T and B lymphocytes. Eur J Immunol. 1973 Jan;3(1):6–9. doi: 10.1002/eji.1830030103. [DOI] [PubMed] [Google Scholar]
  7. Chapel H. M. The effects of papain, trypsin and phospholipase A on rosette formation. Transplantation. 1973 Mar;15(3):320–325. doi: 10.1097/00007890-197303000-00009. [DOI] [PubMed] [Google Scholar]
  8. Charreire J., Bach J. F. Letter: Self and not-self. Lancet. 1974 Aug 3;2(7875):299–300. doi: 10.1016/s0140-6736(74)91474-3. [DOI] [PubMed] [Google Scholar]
  9. Coombs R. R., Gurner B. W., Wilson A. B., Holm G., Lindgren B. Rosette-formation between human lymphocytes and sheep red cells not involving immunoglobulin receptors. Int Arch Allergy Appl Immunol. 1970;39(5-6):658–663. doi: 10.1159/000230390. [DOI] [PubMed] [Google Scholar]
  10. Galili U., Schlesinger M. The formation of stable E rosettes after neuraminidase treatment of either human peripheral blood lymphocytes or of sheep red blood cells. J Immunol. 1974 May;112(5):1628–1634. [PubMed] [Google Scholar]
  11. Han T. Enhancement of in vitro lymphocyte response by neuraminidase. Clin Exp Immunol. 1973 Jan;13(1):165–170. [PMC free article] [PubMed] [Google Scholar]
  12. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lay W. H., Mendes N. F., Bianco C., Nussenzweig V. Binding of sheep red blood cells to a large population of human lymphocytes. Nature. 1971 Apr 23;230(5295):531–532. doi: 10.1038/230531a0. [DOI] [PubMed] [Google Scholar]
  14. Novogrodsky A. Potentiation of phytomitogens action by neuraminidase and basic polypeptides. Nature. 1974 Aug 30;250(5469):788–790. doi: 10.1038/250788a0. [DOI] [PubMed] [Google Scholar]
  15. Ross G. D., Rabellino E. M., Polley M. J., Grey H. M. Combined studies of complement receptor and surface immunoglobulin-bearing cells and sheep erythrocyte rosette-forming cells in normal and leukemic human lymphocytes. J Clin Invest. 1973 Feb;52(2):377–385. doi: 10.1172/JCI107194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sandilands G., Gray K., Cooney A., Browning J. D., Anderson J. R. Letter: Auto-rosette formation by human thymocytes and lymphocytes. Lancet. 1974 Jan 5;1(7845):27–28. doi: 10.1016/s0140-6736(74)93019-0. [DOI] [PubMed] [Google Scholar]
  17. Siegel I., Sherman W. B. The interaction of lymphocytes with autologous red cells. J Allergy Clin Immunol. 1972 Aug;50(2):65–74. doi: 10.1016/0091-6749(72)90001-2. [DOI] [PubMed] [Google Scholar]
  18. Singh J., Elson C. J., Taylor R. B. Enrichment in B cells after separation of lymphocytes from peripheral blood. J Immunol Methods. 1974 Jul;5(2):111–119. doi: 10.1016/0022-1759(74)90001-5. [DOI] [PubMed] [Google Scholar]
  19. Steel C. M., Evans J., Smith M. A. Physiological variation in circulating B cell:T cell ratio in man. Nature. 1974 Feb 8;247(5440):387–389. doi: 10.1038/247387a0. [DOI] [PubMed] [Google Scholar]
  20. Whittingham S., Mackay I. R. Rosette formation by human thymocytes. Cell Immunol. 1973 Mar;6(3):362–367. doi: 10.1016/0008-8749(73)90036-1. [DOI] [PubMed] [Google Scholar]
  21. Woodruff J. J., Gesner B. M. The effect of neuraminidase on the fate of transfused lymphocytes. J Exp Med. 1969 Mar 1;129(3):551–567. doi: 10.1084/jem.129.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]

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