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. 1977 Apr;28(1):89–98.

Cell-mediated immune responses in patients with paracoccidioidomycosis.

P W Mok, D L Greer
PMCID: PMC1540883  PMID: 862251

Abstract

We tested the hypothesis that symptomatic infection with Paracoccidioides brasiliensis caused impaired host cellular immune responses. In a cross-sectional study in Colombia, the immune responses of thirty-six patients with paracoccidioidomycosis were compared with those of sixty normal individuals. Patients demonstrated increased skin sensitivity to paracoccidioidin (para) and histoplasmin, and reduced reactivity to candidin and dinitrochlorobenzene as compared to controls. The skin test response to tuberculin (PPD) was similar to that of controls. In vitro lymphocyte transformation (LT) and leucocyte migration responses to phytohaemagglutinin and PPD did not differ in patients and controls; these responses to PPD correlated with skin sensitivity in controls, but not in patients. LT and inhibition of leucocyte migration to para were seen in more patients than controls; the latter response correlated with skin sensitivity in controls only. Positive LT to para was associated with absence of antibodies to P. brasiliensis. Analysis of symptomatic patients suggests that the prevalence of para skin test positivity was lowest in patients with the longest duration of disease; this implies decrease in specific cell-mediated immunity with prolonged active infection. Analysis of clinically cured patients suggests that the prevalence of para skin sensitivity and LT to para and PPD increased with time elapsed since diagnosis; this implies development or restoration of immunocompetence upon clinical recovery. Results of a preliminary longitudinal study on the immunological responses of six patients with active paracoccidioidomycosis are compatible with the above observations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bice D. E., Lopez M., Rothschild H., Salvaggio J. Comparison of Candida-delayed hypersensitivity skin test size with lymphocyte transformation, migration inhibitory factor production and antibody titer. Int Arch Allergy Appl Immunol. 1974;47(1):54–62. doi: 10.1159/000231200. [DOI] [PubMed] [Google Scholar]
  2. Brown R. S., Haynes H. A., Foley H. T., Godwin H. A., Berard C. W., Carbone P. P. Hodgkin's disease. Immunologic, clinical, and histologic features of 50 untreated patients. Ann Intern Med. 1967 Aug;67(2):291–302. doi: 10.7326/0003-4819-67-2-291. [DOI] [PubMed] [Google Scholar]
  3. Bullock W. E., Fields J. P., Brandriss M. W. An evaluation of transfer factor as immunotherapy for patients with lepromatous leprosy. N Engl J Med. 1972 Nov 23;287(21):1053–1059. doi: 10.1056/NEJM197211232872101. [DOI] [PubMed] [Google Scholar]
  4. Chase M. W. Delayed-type hypersensitivity and the immunology of Hodgkin's disease, with a parallel examination of sarcoidosis. Cancer Res. 1966 Jun;26(6):1097–1120. [PubMed] [Google Scholar]
  5. Deresinski S. C., Levine H. B., Stevens D. A. Soluble antigens of mycelia and spherules in the in vitro detection of immunity to Coccidioides immitis. Infect Immun. 1974 Oct;10(4):700–704. doi: 10.1128/iai.10.4.700-704.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giraldo R., Restrepo A., Gutiérrez F., Robledo M., Londoño F., Hernández H., Sierra F., Calle G. Pathogenesis of paracoccidioidomycosis: a model based on the study of 46 patients. Mycopathologia. 1976 Jun 18;58(2):63–70. doi: 10.1007/BF00707174. [DOI] [PubMed] [Google Scholar]
  7. Graybill J. R., Silva J., Jr, Alford R. H., Thor D. E. Immunologic and clinical improvement of progressive coccidioidomycosis following administration of transfer factor. Cell Immunol. 1973 Jul;8(1):120–135. doi: 10.1016/0008-8749(73)90099-3. [DOI] [PubMed] [Google Scholar]
  8. Greer D. L., D'Costa de Estrada D., Agredo de Trejos L. Dermal sensitivity to paracoccidiodin and histoplasmin in family members of patients with paracoccidioidomycosis. Am J Trop Med Hyg. 1974 Jan;23(1):87–98. doi: 10.4269/ajtmh.1974.23.87. [DOI] [PubMed] [Google Scholar]
  9. Junge U., Hoekstra J., Wolfe L., Deinhardt F. Microtechnique for quantitative evaluation of in vitro lymphocyte transformation. Clin Exp Immunol. 1970 Sep;7(3):431–437. [PMC free article] [PubMed] [Google Scholar]
  10. Mendes E., Raphael A. Impaired delayed hypersensitivity in patients with South American blastomycosis. J Allergy. 1971 Jan;47(1):17–22. doi: 10.1016/s0091-6749(71)80313-5. [DOI] [PubMed] [Google Scholar]
  11. Mendes N. F., Musatti C. C., Leão R. C., Mendes E., Naspitz C. K. Lymphocyte cultures and skin allograft survival in patients with South American blastomycosis. J Allergy Clin Immunol. 1971 Jul;48(1):40–45. doi: 10.1016/0091-6749(71)90054-6. [DOI] [PubMed] [Google Scholar]
  12. Miller S. D., Jones H. E. Correlation of lymphocyte transformation with tuberculin skin-test sensitivity. Am Rev Respir Dis. 1973 Apr;107(4):530–538. doi: 10.1164/arrd.1973.107.4.530. [DOI] [PubMed] [Google Scholar]
  13. Restrepo-Moreno A., Schneidau J. D., Jr Nature of the skin-reactive principle in culture filtrates prepared from Paracoccidioides brasiliensis. J Bacteriol. 1967 Jun;93(6):1741–1748. doi: 10.1128/jb.93.6.1741-1748.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Restrepo A., Robledo M., Gutiérrez F., Sanclemente M., Castañeda E., Calle G. Paracoccidioidomycosis, (South American blastomycosis). A study of 39 cases observed in Medellin, Colombia. Am J Trop Med Hyg. 1970 Jan;19(1):68–76. [PubMed] [Google Scholar]
  15. Restrepo M. A. La prueba de inmunodiffusion en el diagnostico de la paracoccidioidomicosis. Sabouraudia. 1966 Feb;4(4):223–230. [PubMed] [Google Scholar]
  16. Rocklin R. E., Chilgren R. A., Hong R., David J. R. Transfer of cellular hypersensitivity in chronic mucocutaneous candidiasis monitored in vivo and in vitro. Cell Immunol. 1970 Sep;1(3):290–299. doi: 10.1016/0008-8749(70)90050-x. [DOI] [PubMed] [Google Scholar]
  17. Stevens D. A., Levine H. B., TenEyck D. R. Dermal sensitivity to different doses of spherulin and coccidioidin. Chest. 1974 May;65(5):530–533. doi: 10.1378/chest.65.5.530. [DOI] [PubMed] [Google Scholar]
  18. Turk J. L., Bryceson A. D. Immunological phenomena in leprosy and related diseases. Adv Immunol. 1971;13:209–266. doi: 10.1016/s0065-2776(08)60185-6. [DOI] [PubMed] [Google Scholar]

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