Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1977 Jul;29(1):176–180.

Effect of A. laidlawii on murine and human lymphocyte cultures.

H Kirchner, H Brunner, H Rühl
PMCID: PMC1541036  PMID: 891034

Abstract

Heat-inactivated A. laidlawii (AL) was found to be a potent mitogen for mouse spleen cells. Spleen cells from homozygous nude mice and spleen cells treated with anti-theta serum and complement responded as well as their respective controls, indicating that AL represented a B-cell mitogen for mouse spleen cells. Spleen cells from LPS-unresponsive C3H/Hej mice responded well to AL. The peripheral blood leucocytes from unselected human donors were also stimulated by AL, which appeared to represent a T-cell mitogen for human leucocytes. However, the possibility that it acted as a specific antigen could not be excluded. Attention was drawn to the possibility that the presence of mycoplasma might considerably affect the results of tests where tissue-culture cells or derivatives thereof are added to leucocyte cultures.

Full text

PDF
177

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisenberg A. C., Long J. C., Wilkes B. Chronic lymphocytic leukemia cells: rosette formation and adherence to nylon fiber columns. J Natl Cancer Inst. 1974 Jan;52(1):13–17. doi: 10.1093/jnci/52.1.13. [DOI] [PubMed] [Google Scholar]
  2. Biberfeld G., Biberfeld P., Sterner G. Cell-mediated immune response following Mycoplasma pneumoniae infection in man. I. Lymphocyte stimulation. Clin Exp Immunol. 1974 May;17(1):29–41. [PMC free article] [PubMed] [Google Scholar]
  3. Biberfeld G., Gronowicz E. Mycoplasma pneumoniae is a polyclonal B-cell activator. Nature. 1976 May 20;261(5557):238–239. doi: 10.1038/261238a0. [DOI] [PubMed] [Google Scholar]
  4. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Copperman R., Morton H. E. Reversible inhibition of mitosis in lymphocyte cultures by non-viable Mycoplasma. Proc Soc Exp Biol Med. 1966 Dec;123(3):790–795. doi: 10.3181/00379727-123-31605. [DOI] [PubMed] [Google Scholar]
  6. Dörner I., Brunner H., Schiefer H. G., Wellensiek H. J. Complement-mediated killing of Acholeplasma laidlawii by antibodies to various membrane components. Infect Immun. 1976 Jun;13(6):1663–1670. doi: 10.1128/iai.13.6.1663-1670.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fernald G. W. In vitro response of human lymphocytes to Mycoplasma pneumoniae. Infect Immun. 1972 Apr;5(4):552–558. doi: 10.1128/iai.5.4.552-558.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ginsburg H., Nicolet J. Extensive transformation of lymphocytes by a mycoplasma organism. Nat New Biol. 1973 Dec 5;246(153):143–146. doi: 10.1038/newbio246143a0. [DOI] [PubMed] [Google Scholar]
  9. Holden H. T., Kirchner H., Herberman R. B. Secondary cell-mediated cytotoxic response to syngeneic mouse tumor challenge. J Immunol. 1975 Aug;115(2):327–331. [PubMed] [Google Scholar]
  10. Kirchner H., Herberman R. B., Glaser M., Lavrin D. H. Suppression of in vitro lymphocyte stimulation in mice bearing primary Moloney sarcoma virus-induced tumors. Cell Immunol. 1974 Jul;13(1):32–40. doi: 10.1016/0008-8749(74)90224-x. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Morrison D. C., Betz S. J., Jacobs D. M. Isolation of a lipid A bound polypeptide responsible for "LPS-initiated" mitogenesis of C3H/HeJ spleen cells. J Exp Med. 1976 Sep 1;144(3):840–846. doi: 10.1084/jem.144.3.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pang G. T., Baguley D. M., Wilson J. D. Spontaneous rosettes as a T-lymphocyte marker: a modified method giving consistent results. SRBC rosettes. J Immunol Methods. 1974 Jan;3(1):41–46. doi: 10.1016/0022-1759(74)90031-3. [DOI] [PubMed] [Google Scholar]
  14. Peavy D. L., Adler W. H., Smith R. T. The mitogenic effects of endotoxin and staphylococcal enterotoxin B on mouse spleen cells and human peripheral lymphocytes. J Immunol. 1970 Dec;105(6):1453–1458. [PubMed] [Google Scholar]
  15. Wyler D. J., Oppenheim J. J. Lymphocyte transformation in human Plasmodium falciparum malaria. J Immunol. 1974 Aug;113(2):449–454. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES