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. 1977 Dec;30(3):439–449.

Kidney lesions in baboons infected with Schistosoma mansoni.

V Houba, R F Sturrock, A E Butterworth
PMCID: PMC1541142  PMID: 414868

Abstract

Glomerular lesions in baboons (Papio anubis) infected with different dosage regimes of Schistosoma mansoni were studied by immunofluorescence and light microscopy on kidney sections and by countercurrent immunoelectrophoresis on kidney homogenates and tissue eluates. Mild lesions, characterized by focal and segmental deposits of immune complexes, developed in sixty-two out of 103 baboons, irrespective of the intensity and duration of the infection. Severe, diffuse lesions developed in six baboons after prolonged and heavy infections. Adult worm and soluble egg antigens, together with IgM, IgG and C3, were detected in most of the severe lesions and in some of the mild lesions. In some animals, antigens were detected in most of the severe lesions and in some of the mild lesions. In some animals, antigens were detected in acid homogenates and eluates of kidneys which showed no deposits of immunoglobulins or complement. These observations indicate that renal lesions in S. mansoni infections may be attributable to the deposition of immune complexes pre-formed in the circulation. However, the demonstration of antigens alone in some animals may suggest an alternative possibility, namely that antigens are deposited first with a subsequent binding of antibody and complement.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baum J. Naturally occurring haemagglutinins in the New Zealand black mouse. Clin Exp Immunol. 1969 Apr;4(4):453–456. [PMC free article] [PubMed] [Google Scholar]
  2. Berggren W. L., Weller T. H. Immunoelectrophoretic demonstration of specific circulating antigen in animals infected with Schistosoma mansoni. Am J Trop Med Hyg. 1967 Sep;16(5):606–612. doi: 10.4269/ajtmh.1967.16.606. [DOI] [PubMed] [Google Scholar]
  3. Bhamarapravati N., Boonpucknavig S., Boonpucknavig V., Yaemboonruang C. Glomerular changes in acute plasmodium falciparum infection. An immunopathologic study. Arch Pathol. 1973 Nov;96(5):289–293. [PubMed] [Google Scholar]
  4. Boonpucknavig S., Boonpucknavig V., Bhamarapravati N. Immunopathological studies of Plasmodium berghei-infected mice. Immune complex nephritis. Arch Pathol. 1972 Oct;94(4):322–330. [PubMed] [Google Scholar]
  5. Brandtzaeg P. Rhodamine conjugates:specific and nonspecific binding properties in immunohistochemistry. Ann N Y Acad Sci. 1975 Jun 30;254:35–54. doi: 10.1111/j.1749-6632.1975.tb29154.x. [DOI] [PubMed] [Google Scholar]
  6. Butterworth A. E., Sturrock R. F., Houba V., Taylor R. Schistosoma mansoni in baboons. Antibody-dependent cell-mediated damage to 51Cr-labelled schistosomula. Clin Exp Immunol. 1976 Jul;25(1):95–102. [PMC free article] [PubMed] [Google Scholar]
  7. CLARK H. F., SHEPARD C. C. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY. Virology. 1963 Aug;20:642–644. doi: 10.1016/0042-6822(63)90292-7. [DOI] [PubMed] [Google Scholar]
  8. Carlier Y., Bout D., Bina J. C., Camus D., Figueiredo J. F., Capron A. Immunological studies in human schistosomiasis. I. Parasitic antigen in urine. Am J Trop Med Hyg. 1975 Nov;24(6 Pt 1):949–954. doi: 10.4269/ajtmh.1975.24.949. [DOI] [PubMed] [Google Scholar]
  9. Cavallo T., Galvanek E. G., Ward P. A., von Lichtenberg F. The nephropathy of experimental hepatosplenic schistosomiasis. Am J Pathol. 1974 Sep;76(3):433–450. [PMC free article] [PubMed] [Google Scholar]
  10. Cheever A. W. Conditions affecting the accuracy of potassium hydroxide digestion techniques for counting Schistosoma mansoni eggs in tissues. Bull World Health Organ. 1968;39(2):328–331. [PMC free article] [PubMed] [Google Scholar]
  11. DIXON F. J. RENAL INJURY INDUCED BY ANTIGEN-ANTIBODY COMPLEXES AND OTHER IMMUNOLOGIC MEANS. Fed Proc. 1965 Jan-Feb;24:98–99. [PubMed] [Google Scholar]
  12. De Brito T., Gunji J., Camargo M. E., Ceravolo A., Da Silva L. C. Glomerular lesions in experimental infections of Schistosoma mansoni in Cebus apella monkeys. Bull World Health Organ. 1971;45(4):419–422. [PMC free article] [PubMed] [Google Scholar]
  13. Deelder A. M., Klappe H. T., van den Aardweg G. J., van Meerbeke E. H. Schistosoma mansoni: demonstration of two circulating antigens in infected hamsters. Exp Parasitol. 1976 Oct;40(2):189–197. doi: 10.1016/0014-4894(76)90081-3. [DOI] [PubMed] [Google Scholar]
  14. Ehrich J. H., Voller A. Studies on the kidneys of mice infected with rodent malaria. I. Deposition of gamma-globulins in glomeruli in the early stage of the disease. Z Tropenmed Parasitol. 1972 Jun;23(2):147–152. [PubMed] [Google Scholar]
  15. Gold R., Rosen F. S., Weller T. H. A specific circulating antigen in hamsters infected with Schistosoma mansoni. Detection of antigen in serum and urine, and correlation between antigenic concentration and worm burden. Am J Trop Med Hyg. 1969 Jul;18(4):545–552. doi: 10.4269/ajtmh.1969.18.545. [DOI] [PubMed] [Google Scholar]
  16. Higashi G. I., Farid Z., Bassily S., Miner W. F. Nephrotic syndrome in Schistosomiasis mansoni complicated by chronic salmonellosis. Am J Trop Med Hyg. 1975 Jul;24(4):713–714. doi: 10.4269/ajtmh.1975.24.713. [DOI] [PubMed] [Google Scholar]
  17. Hillyer G. V., Campos J. A., Lluberes R., Cangiano J. L. Schistosomal nephropathy? I. Preliminary studies of a patient with schistosomiasis mansoni and glomerulonephritis in Puerto Rico. Bol Asoc Med P R. 1975 Nov;67(11):339–344. [PubMed] [Google Scholar]
  18. Hillyer G. V. Deoxyribonucleic acid (DNA) and antibodies to DNA in the serum of hamsters and man infected with schistosomes. Proc Soc Exp Biol Med. 1971 Mar;136(3):880–883. doi: 10.3181/00379727-136-35386. [DOI] [PubMed] [Google Scholar]
  19. Hillyer G. V., Lewert R. M. Studies on renal pathology in hamsters infected with Schistosoma mansoni and S. japonicum. Am J Trop Med Hyg. 1974 May;23(3):404–411. doi: 10.4269/ajtmh.1974.23.404. [DOI] [PubMed] [Google Scholar]
  20. Hoshino-Shimizu S., De Brito T., Kanamura H. Y., Canto A. L., Silva A. O., Campos A. R., Penna D. O., Da Silva L. C. Human schistosomiasis: Schistosoma mansoni antigen detection in renal glomeruli. Trans R Soc Trop Med Hyg. 1976;70(5-6):492–496. doi: 10.1016/0035-9203(76)90135-8. [DOI] [PubMed] [Google Scholar]
  21. Houba V., Allison A. C., Adeniyi A., Houba J. E. Immunoglobulin classes and complement in biopsies of Nigerian children with the nephrotic syndrome. Clin Exp Immunol. 1971 May;8(5):761–774. [PMC free article] [PubMed] [Google Scholar]
  22. Houba V., Koech D. K., Sturrock R. F., Butterworth A. E., Kusel J. R., Mahmoud A. A. Soluble antigens and antibodies in sera from baboons infected with Schistosoma mansoni. J Immunol. 1976 Aug;117(2):705–707. [PubMed] [Google Scholar]
  23. Izui S., Lambert P. H., Miescher P. A. Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent. Clin Exp Immunol. 1976 Dec;26(3):425–430. [PMC free article] [PubMed] [Google Scholar]
  24. Koffler D., Schur P. H., Kunkel H. G. Immunological studies concerning the nephritis of systemic lupus erythematosus. J Exp Med. 1967 Oct 1;126(4):607–624. doi: 10.1084/jem.126.4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Lambert P. H., Bricteux N., Salmon J., Miescher P. A. Dynamics of immune complex nephritis during antibody excess. Int Arch Allergy Appl Immunol. 1973;45(1):185–189. doi: 10.1159/000231026. [DOI] [PubMed] [Google Scholar]
  26. Madwar M. A., Voller A. Circulating soluble antigens and antibody in schistosomiasis. Br Med J. 1975 Feb 22;1(5955):435–436. doi: 10.1136/bmj.1.5955.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Milgrom F., Campbell W. A., Andres G. A. Antigen in immune complex nephritis. V. Recovery and identification by gel precipitation. Immunology. 1976 Feb;30(2):277–280. [PMC free article] [PubMed] [Google Scholar]
  28. Nash T. E. Localization of the circulating antigen within the gut of Schistosoma mansoni. Am J Trop Med Hyg. 1974 Nov;23(6):1085–1087. doi: 10.4269/ajtmh.1974.23.1085. [DOI] [PubMed] [Google Scholar]
  29. Nash T. E., Prescott B., Neva F. A. The characteristics of a circulating antigen in schistosomiasis. J Immunol. 1974 Apr;112(4):1500–1507. [PubMed] [Google Scholar]
  30. Natali P. G., Cioli D. Immune complex nephritis in Schistosoma mansoni-infected mice. Eur J Immunol. 1976 May;6(5):359–364. doi: 10.1002/eji.1830060511. [DOI] [PubMed] [Google Scholar]
  31. Neoh S. H., Jahoda D. M., Rowe D. S., Voller A. Immunoglobulin classes in mammalian species identifed by cross-reactivity with antisera to human immunoglobulin. Immunochemistry. 1973 Dec;10(12):805–813. doi: 10.1016/0019-2791(73)90184-5. [DOI] [PubMed] [Google Scholar]
  32. Nydegger U. E., Lambert P. H., Gerber H., Miescher P. A. Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of hepatitis B antigen. Quantitation by binding to radiolabeled C1q. J Clin Invest. 1974 Aug;54(2):297–309. doi: 10.1172/JCI107765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Phillips T. M., Draper C. C. Circulating immune complexes in schistosomiasis due to Schistosoma mansoni. Br Med J. 1975 May 31;2(5969):476–477. doi: 10.1136/bmj.2.5969.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Queiroz P. F., Brito E., Martinelli R., Rocha H. Nephrotic syndrome in patients with Schistosoma mansoni infection. Am J Trop Med Hyg. 1973 Sep;22(5):622–628. doi: 10.4269/ajtmh.1973.22.622. [DOI] [PubMed] [Google Scholar]
  35. Rocha H., Cruz T., Brito E., Susin M. Renal involvement in patients with hepatosplenic Schistosomiasis mansoni. Am J Trop Med Hyg. 1976 Jan;25(1):108–115. doi: 10.4269/ajtmh.1976.25.108. [DOI] [PubMed] [Google Scholar]
  36. Santoro F., Bout D., Capron A. Imuno-complexos na esquistossomose. II. Dosagem radio-immunológica da ligaço do Clq-I125 ao IC. Rev Inst Med Trop Sao Paulo. 1976 Sep-Oct;18(5):293–297. [PubMed] [Google Scholar]
  37. Santoro F., Bout D., Wattre P., Capron A. Imuno-complexos na esquistossomose. I. Utilizaço da fixaço do complemento para sua detecço. Rev Inst Med Trop Sao Paulo. 1976 May-Jun;18(3):152–156. [PubMed] [Google Scholar]
  38. Sturrock R. F., Butterworth A. E., Houba V. Schistosoma mansoni in the baboon (Papio anubis): parasitological responses of Kenyan baboons to different exposures of a local parasite strain. Parasitology. 1976 Dec;73(3):239–252. doi: 10.1017/s003118200004693x. [DOI] [PubMed] [Google Scholar]
  39. Tada T., Kondo Y., Okumura K., Sano M., Yokogawa M. Schistosoma japonicum: immunopathology of nephritis in Macaca fascicularis. Exp Parasitol. 1975 Dec;38(3):291–302. doi: 10.1016/0014-4894(75)90114-9. [DOI] [PubMed] [Google Scholar]
  40. Ward P. A., Conran P. B. Immunopathology of renal complications in simian malaria and human quartan malaria. Mil Med. 1969 Sep;134(10):1228–1236. [PubMed] [Google Scholar]
  41. Wilson M., Sulzer A. J., Walls K. W. Modified antigens in the indirect immunofluorescence test for schistosomiasis. Am J Trop Med Hyg. 1974 Nov;23(6):1072–1076. doi: 10.4269/ajtmh.1974.23.1072. [DOI] [PubMed] [Google Scholar]
  42. da Silva L. C., de Brito T., Camargo M. E., de Boni D. R., Lopes J. D., Gunji J. Kidney biopsy in the hepatosplenic form of infection with Schistosoma mansoni in man. Bull World Health Organ. 1970;42(6):907–910. [PMC free article] [PubMed] [Google Scholar]
  43. von Lichtenberg F., Bawden M. P., Shealey S. H. Origin of circulating antigen from the schistosome gut. An immunofluorescent study. Am J Trop Med Hyg. 1974 Nov;23(6):1088–1091. doi: 10.4269/ajtmh.1974.23.1088. [DOI] [PubMed] [Google Scholar]

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