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. 1978 Jan;31(1):92–99.

Antibodies to gluten and reticulin in gastrointestinal diseases

K P Eterman, T E W Feltkamp
PMCID: PMC1541185  PMID: 639353

Abstract

Antibodies to reticulin were found in 33% of coeliac patients on a normal diet. These antibodies were found in only 11% of coeliac patients on a gluten-free diet. In patients with dermatitis herpitiformis, 12% had these antibodies, whereas the highest frequency in the other diseases studied (Crohn's disease, ulcerative colitis, cystic fibrosis and `recurrent diarrhoea') was 7% (compared to 2% in healthy controls).

Antibodies to gluten, demonstrated with the immunofluorescence technique, were found in all coeliac children on a normal diet that were studied, and in half of the adults with the untreated disease. In children and adults on a gluten-free diet these frequencies decreased to 87 and 32%. In Crohn's disease, cystic fibrosis, recurrent diarrhoea, dermatitis herpetiformis and ulcerative colitis, the frequencies were 52, 42, 37, 18 and 18%, respectively (and in 4% of controls).

It was therefore concluded that antibodies to gluten were sensitive markers for gastrointestinal diseases, but were not specific for gluten enteropathy. Antibodies to reticulin, on the other hand, were less sensitive but of far greater specificity for coeliac disease. Gluten antibodies were of the IgA, IgM and IgG classes, whereas antibodies to reticulin were only of the IgA and IgG classes. Both types of antibody were found to be non-complement-fixing. Autoantibodies to smooth muscle were found in 5% of the coeliac patients (and in 0% of controls). No relationship with hepatic complications was found. In the patients with dermatitis herpetiformis, autoantibodies to gastric parietal cells were found in 24% (and in 5% of controls). No relationship was established between the occurrence of HLA-B8 and the presence or absence of any of the antibodies studied.

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Selected References

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  1. ALARCON SEGOVIA D., HERSKOVIC T., WAKIM K. G., GREEN P. A., SCUDAMORE H. H. PRESENCE OF CIRCULATING ANTIBODIES TO GLUTEN AND MILK FRACTIONS IN PATIENTS WITH NONTROPICAL SPRUE. Am J Med. 1964 Apr;36:485–499. doi: 10.1016/0002-9343(64)90098-1. [DOI] [PubMed] [Google Scholar]
  2. Alp M. H., Wright R. Autoantibodies to reticulin in patients with idiopathic steatorrhoea, coeliac disease, and Crohn's disease, and their relation to immunoglobulins and dietary antibodies. Lancet. 1971 Sep 25;2(7726):682–685. doi: 10.1016/s0140-6736(71)92249-5. [DOI] [PubMed] [Google Scholar]
  3. BERGER E., BUERGIN-WOLFF A., FREUDENBERG E. DIAGNOSTISCHE BEWERTUNG DES NACHWEISES VON GLIADIN-ANTIKOERPERN BEI COELIAKIE. Klin Wochenschr. 1964 Aug 15;42:788–790. doi: 10.1007/BF01479129. [DOI] [PubMed] [Google Scholar]
  4. Carswell F., Ferguson A. Plasma food antibodies during withdrawal and reintroduction of dietary gluten in coeliac disease. Arch Dis Child. 1973 Aug;48(8):583–586. doi: 10.1136/adc.48.8.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DONIACH I., SHINER M. Duodenal and jejunal biopsies. II. Histology. Gastroenterology. 1957 Jul;33(1):71–86. [PubMed] [Google Scholar]
  6. Di Sant'Agnese P. A., Davis P. B. Research in cystic fibrosis (first of three parts). N Engl J Med. 1976 Aug 26;295(9):481–485. doi: 10.1056/NEJM197608262950905. [DOI] [PubMed] [Google Scholar]
  7. Eterman K. P., Hekkens W. T., Pena A. S., Lems-van Kan P. H., Feltkamp T. E. Wheat grains: a substrate for the determination of gluten antibodies in serum of gluten-sensitive patients. J Immunol Methods. 1977;14(1):85–92. doi: 10.1016/s0022-1759(97)90025-9. [DOI] [PubMed] [Google Scholar]
  8. Falchuk K. R., Isselbacher K. J. Circulating antibodies to bovine albumin in ulcerative colitis and Crohn's disease. Characterization of the antibody response. Gastroenterology. 1976 Jan;70(1):5–8. [PubMed] [Google Scholar]
  9. Falchuk Z. M., Strober W. Gluten-sensitive enteropathy: synthesis of antigliadin antibody in vitro. Gut. 1974 Dec;15(12):947–952. doi: 10.1136/gut.15.12.947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feltkamp T. E., van Rossum A. L. Antibodies to salivary duct cells, and other autoantibodies, in patients with Sjögren's syndrome and other idiopathic autoimmune diseases. Clin Exp Immunol. 1968 Jan;3(1):1–16. [PMC free article] [PubMed] [Google Scholar]
  11. Ferguson A., Carswell F. Precipitins to dietary proteins in serum and upper intestinal secretions of coeliac children. Br Med J. 1972 Jan 8;1(5792):75–77. doi: 10.1136/bmj.1.5792.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goodchild M. C., Nelson R., Anderson C. M. Cystic fibrosis and coeliac disease: coexistence in two children. Arch Dis Child. 1973 Sep;48(9):684–691. doi: 10.1136/adc.48.9.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. James A. H. Breakfast and Crohn's disease. Br Med J. 1977 Apr 9;1(6066):943–945. doi: 10.1136/bmj.1.6066.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lancaster-Smith M., Packer S., Kumar P. J., Harries J. T. Immunological phenomena in the jejunum and serum after reintroduction of dietary gluten in children with treated coeliac disease. J Clin Pathol. 1976 Jul;29(7):592–597. doi: 10.1136/jcp.29.7.592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lucas C. J., Brouwer R., Feltkamp T. E., ten Veen J. H., van Loghem J. J. Measles antibodies in sera from patients with autoimmune diseases. Lancet. 1972 Jan 15;1(7742):115–116. doi: 10.1016/s0140-6736(72)90679-4. [DOI] [PubMed] [Google Scholar]
  16. Magalhaes A. F., Peters T. J., Doe W. F. Studies on the nature and significance of connective tissue antibodies in adult coeliac disease and Crohn's disease. Gut. 1974 Apr;15(4):284–288. doi: 10.1136/gut.15.4.284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. O'Donoghue D. P., Lancaster-Smith M., Johnson G. D., Kumar P. J. Gastric lesion in dermatitis herpetiformis. Gut. 1976 Mar;17(3):185–188. doi: 10.1136/gut.17.3.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. POKORNA M., SOUREK J., SVEJCAR J. DIE BEDEUTUNG DER PRAEZIPITINE GEGENUEBER GLIADIN IM SERUM VON KINDERN MIT COELIAKIE. Helv Paediatr Acta. 1963 Dec;18:393–397. [PubMed] [Google Scholar]
  19. Pras M., Glynn L. E. Isolation of a non-collagenous reticulin component and its primary characterization. Br J Exp Pathol. 1973 Aug;54(4):449–456. [PMC free article] [PubMed] [Google Scholar]
  20. Rizzetto M., Doniach D. Types of 'reticulin' antibodies detected in human sera by immunofluorescence. J Clin Pathol. 1973 Nov;26(11):841–851. doi: 10.1136/jcp.26.11.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Savilahti E. Intestinal immunoglobulins in children with coeliac disease. Gut. 1972 Dec;13(12):958–964. doi: 10.1136/gut.13.12.958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Seah P. P., Fry L., Hoffbrand A. V., Holborow E. J. Tissue antibodies in dermatitis herpetiformis and adult coeliac disease. Lancet. 1971 Apr 24;1(7704):834–836. doi: 10.1016/s0140-6736(71)91499-1. [DOI] [PubMed] [Google Scholar]
  23. Seah P. P., Fry L., Holborow E. J., Rossiter M. A., Doe W. F., Magalhaes A. F., Hoffbrand A. V. Antireticulin antibody: incidence and diagnostic significance. Gut. 1973 Apr;14(4):311–315. doi: 10.1136/gut.14.4.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Seah P. P., Fry L., Rossiter M. A., Hoffbrand A. V., Holborow E. J. Anti-reticulin antibodies in childhood coeliac disease. Lancet. 1971 Sep 25;2(7726):681–682. doi: 10.1016/s0140-6736(71)92248-3. [DOI] [PubMed] [Google Scholar]
  25. Sokol G., Taylor B. Cystic fibrosis and coeliac disease. Report of two cases. Arch Dis Child. 1973 Sep;48(9):692–696. doi: 10.1136/adc.48.9.692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stevens F. M., Lloyd R., Egan-Mitchell B., Mylotte M. J., Fottrell P. F., Wright R., McNicholl B., McCarthy C. F. Reticulin antibodies in patients with coeliac disease and their relatives. Gut. 1975 Aug;16(8):598–602. doi: 10.1136/gut.16.8.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stokes P. L., Asquith P., Holmes G. K., Mackintosh P., Cooke W. T. Histocompatibility antigens associated with adult coeliac disease. Lancet. 1972 Jul 22;2(7769):162–164. doi: 10.1016/s0140-6736(72)91330-x. [DOI] [PubMed] [Google Scholar]
  28. TAYLOR K. B., TRUELOVE S. C., THOMSON D. L., WRIGHT R. An immunological study of coeliac disease and idiopathic steatorrhoea. Serological reactions to gluten and milk proteins. Br Med J. 1961 Dec 30;2(5269):1727–1731. doi: 10.1136/bmj.2.5269.1727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. TAYLOR K. B., TRUELOVE S. C., WRIGHT R. SEROLOGIC REACTIONS TO GLUTEN AND COW'S MILK PROTEINS IN GASTROINTESTINAL DISEASE. Gastroenterology. 1964 Feb;46:99–108. [PubMed] [Google Scholar]
  30. TERASAKI P. I., MCCLELLAND J. D. MICRODROPLET ASSAY OF HUMAN SERUM CYTOTOXINS. Nature. 1964 Dec 5;204:998–1000. doi: 10.1038/204998b0. [DOI] [PubMed] [Google Scholar]
  31. Williamson N., Asquith P., Stokes L., Jowett W., Cooke W. T. Anticonnective tissue and other antitissue 'antibodies' in the sera of patients with coeliac disease compared with the findings in a mixed hospital population. J Clin Pathol. 1976 Jun;29(6):484–494. doi: 10.1136/jcp.29.6.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. von Essen R., Savilahti E., Pelkonen P. Reticulin antibody in children with malabsorption. Lancet. 1972 May 27;1(7761):1157–1159. doi: 10.1016/s0140-6736(72)91377-3. [DOI] [PubMed] [Google Scholar]

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