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. 1978 Mar;31(3):367–381.

Immunoglobulin systems of human tonsils. I. Control subjects of various ages: qualification of Ig-producing cells, tonsillar morphometry and serum Ig concentrations

P Brandtzaeg, L Surjan, P Berdal
PMCID: PMC1541246  PMID: 350457

Abstract

Specimens of clinically normal palatine tonsils were studied by morphometry and immunohistochemistry, with regard to the relative tissue contribution and the content of Ig-producing immunocytes of four morphological compartments: the germinal centres of lymphoid follicles, their mantle zones, the extrafollicular area and the reticular parts of the crypt epithelium. Ig-producing cells occurred in all compartments; most of them were located in the extrafollicular area, although their density was highest in the reticular epithelium. There was a general predominance of IgG cells—including the blasts present in germinal centres. In subjects 4–25 years old, the tonsillar immunocyte population showed overall IgG:IgA:IgM:IgD class ratios of 65·2:30·1:3·5:1·2. IgE-producing cells were virtually absent.

A reticular distribution of non-diffusible immunoglobulins, especially IgM, was observed in the germinal centres—apparently bound to dendritic reticular cells. The mantle zones commonly contained numerous lymphocytes with membrane-related immunofluorescence, particularly prominent for IgD and less distinct for IgM. These B-cells were probably derived from local clonal expansion processes. There was no indication of active immunoglobulin transport through the tonsillar epithelium, which was devoid of `secretory component'.

In subjects 30–81 years old, lymphoid elements of the tonsils were reduced, especially the follicular mantles and the reticular crypt epithelium, as well as their content of Ig-producing immunocytes. Such cells were also fewer in the germinal centres and in the extrafollicular area. Moreover, some shifts in the immunocyte class ratios had occurred in the various tissue compartments. In this age group, the number of tonsillar IgA cells showed a significant negative correlation with the rate of synthesis of serum IgA.

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Selected References

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  1. Awaya K. The aging of the secondary nodules in relation to the postnatal development and involution of the human palatine tonsils. Nihon Ketsueki Gakkai Zasshi. 1969 Feb;32(1):127–134. [PubMed] [Google Scholar]
  2. Baklien K., Brandtzaeg P. Immunohistochemical characterization of local immunoglobulin formation in Crohn's disease of the ileum. Scand J Gastroenterol. 1976;11(5):447–457. [PubMed] [Google Scholar]
  3. Brandtzaeg P., Baklien K. Inconclusive immunohistochemistry of human IgE in mucosal pathology. Lancet. 1976 Jun 12;1(7972):1297–1298. doi: 10.1016/s0140-6736(76)91767-0. [DOI] [PubMed] [Google Scholar]
  4. Brandtzaeg P. Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique. Immunology. 1974 Jun;26(6):1101–1114. [PMC free article] [PubMed] [Google Scholar]
  5. Brandtzaeg P. Studies on J chain and binding site for secretory component in circulating human B cells. Clin Exp Immunol. 1976 Jul;25(1):50–58. [PMC free article] [PubMed] [Google Scholar]
  6. Cooper M. D., Kearney J. F., Lawton A. R., Abney E. R., Parkhouse R. M., Preud'homme J. L., Seligmann M. Generation of immunoglobulin class diversity in b cells: a discussion with emphasis on idg development. Ann Immunol (Paris) 1976 Jun-Jul;127(3-4):573–581. [PubMed] [Google Scholar]
  7. Crabbé P. A., Heremans J. F. Distribution in human nasopharyngeal tonsils of plasma cells containing different types of immunoglobulin polypeptide chains. Lab Invest. 1967 Jan;16(1):112–123. [PubMed] [Google Scholar]
  8. FeltkampVroom T. M., Stallman P. J., Aalberse R. C., Reerink-Brongers E. E. Immunofluorescence studies on renal tissue, tonsils, adenoids, nasal polyps, and skin of atopic and nonatopic patients, with special reference to IgE. Clin Immunol Immunopathol. 1975 Sep;4(3):392–404. doi: 10.1016/0090-1229(75)90008-2. [DOI] [PubMed] [Google Scholar]
  9. Ferrarini M., Viale G., Risso A., Pernis B. A study of immunoglobulin classes present on the membrane and in the cytoplasm of human tonsil plasma cells. Eur J Immunol. 1976 Aug;6(8):562–565. doi: 10.1002/eji.1830060807. [DOI] [PubMed] [Google Scholar]
  10. Gitlin D., Sasaki T. Immunoglobulins G, A, and M determined in single cells from human tonsil. Science. 1969 Jun 27;164(3887):1532–1534. doi: 10.1126/science.164.3887.1532. [DOI] [PubMed] [Google Scholar]
  11. Hirokawa K., Esaki Y., Nariuchi H. Role of germinal center in immune response enzyme immuno-histochemical study using horseradish peroxidase. Acta Pathol Jpn. 1973 Nov;23(4):739–753. doi: 10.1111/j.1440-1827.1973.tb02772.x. [DOI] [PubMed] [Google Scholar]
  12. Ishikawa T., Wicher K., Arbesman C. E. Distribution of immunoglobulins in palatine and pharyngeal tonsils. Int Arch Allergy Appl Immunol. 1972;43(6):801–812. doi: 10.1159/000230898. [DOI] [PubMed] [Google Scholar]
  13. Janossy G., Gomez de la Concha E., Luquetti A., Snajdr M. J., Waxdal M. J., Platts-Mills T. A. T-cell regulation of immunoglobulin synthesis and proliferation in pokeweed (Pa-1)-stimulated human lymphocyte cultures. Scand J Immunol. 1977;6(1-2):109–123. doi: 10.1111/j.1365-3083.1977.tb00326.x. [DOI] [PubMed] [Google Scholar]
  14. Ogra P. L. Effect of tonsillectomy and adenoidectomy on nasopharyngeal antibody response to poliovirus. N Engl J Med. 1971 Jan 14;284(2):59–64. doi: 10.1056/NEJM197101142840201. [DOI] [PubMed] [Google Scholar]
  15. Oláh I., Everett N. B. Surface epithelium of the rabbit palatine tonsil: scanning and transmission electron microscopic study. J Reticuloendothel Soc. 1975 Jul;18(1):53–62. [PubMed] [Google Scholar]
  16. Oláh I., Törö Effect of neonatal thymectomy on rabbit tonsils. Acta Morphol Acad Sci Hung. 1975;23(3):205–216. [PubMed] [Google Scholar]
  17. Pesák V. The localization of IgA and IgE globulins in the palatinal tonsils, nasal mucous membrane and nasal polypi. Folia Microbiol (Praha) 1971;16(4):323–325. doi: 10.1007/BF02872814. [DOI] [PubMed] [Google Scholar]
  18. Platts-Mills T. A., Ishizaka K. IgA and IgA diphtheria antitoxin responses from human tonsil lymphocytes. J Immunol. 1975 Mar;114(3):1058–1064. [PubMed] [Google Scholar]
  19. Ricci V., Russolo M. Uber die Produktion und die Bedeutung der Immunglobuline G,A,M im Lymphgewebe der Tonsillen. Arch Klin Exp Ohren Nasen Kehlkopfheilkd. 1970;196(2):97–102. [PubMed] [Google Scholar]
  20. Rossen R. D., Morgan C., Hsu K. C., Butler W. T., Rose H. M. Localization of 11 S external secretory IgA by immunofluorescence in tissues lining the oral and respiratory passages in man. J Immunol. 1968 Apr;100(4):706–717. [PubMed] [Google Scholar]
  21. Schmedtje J. F., Batts A. F. Immunoglobulins and blood vessels in the tonsillar crypt epithelium. Ann Otol Rhinol Laryngol. 1973 May-Jun;82(3):359–369. doi: 10.1177/000348947308200316. [DOI] [PubMed] [Google Scholar]
  22. Siegel G., Wilke J. Concentration of immunoglobulins in the tonsils. ORL J Otorhinolaryngol Relat Spec. 1976;38(4):246–251. doi: 10.1159/000275280. [DOI] [PubMed] [Google Scholar]
  23. Smith R. S., Sherman N. A., Newcomb R. W. Synthesis and secretion of immunoglobulin, including IgA, by human tonsil and adenoid tissue cultured in vitro. Int Arch Allergy Appl Immunol. 1974;46(5):785–801. doi: 10.1159/000231177. [DOI] [PubMed] [Google Scholar]
  24. Surjan L., Jr, Brandtzaeg P., Berdal P. Immunoglobulin systems of human tonsils. II. Patients with chronic tonsillitis or tonsillar hyperplasia: quantification of Ig-producing cells, tonsillar morphometry and serum Ig concentrations. Clin Exp Immunol. 1978 Mar;31(3):382–390. [PMC free article] [PubMed] [Google Scholar]
  25. Tada T., Ishizaka K. Distribution of gamma E-forming cells in lymphoid tissues of the human and monkey. J Immunol. 1970 Feb;104(2):377–387. [PubMed] [Google Scholar]
  26. Takeuchi Y., Tabata T., Kitashoji N. Localization of E and EAC binding lymphocytes in human tonsils. ORL J Otorhinolaryngol Relat Spec. 1976;38(2):99–107. doi: 10.1159/000275263. [DOI] [PubMed] [Google Scholar]
  27. Tolhurst D. J. Colour-coding properties of sustained and transient channels in human vision. Nature. 1977 Mar 17;266(5599):266–268. doi: 10.1038/266266a0. [DOI] [PubMed] [Google Scholar]
  28. Turesson I. Distribution of immunoglobulin-containing cells in human bone marrow and lymphoid tissues. Acta Med Scand. 1976;199(4):293–304. doi: 10.1111/j.0954-6820.1976.tb06735.x. [DOI] [PubMed] [Google Scholar]
  29. Waldmann T. A., Strober W. Metabolism of immunoglobulins. Prog Allergy. 1969;13:1–110. doi: 10.1159/000385919. [DOI] [PubMed] [Google Scholar]
  30. Watanabe T., Yoshizaki K., Yagura T., Yamamura Y. In vitro antibody formation by human tonsil lymphocytes. J Immunol. 1974 Aug;113(2):608–616. [PubMed] [Google Scholar]
  31. Willson J. K., Jr, Zaremba J. L., Pitts A. M., Pretlow T. G., 2nd A characterization of human tonsillar lymphocytes after separation from other tonsillar cells in an isokinetic gradient of ficoll in tissue culture medium. Am J Pathol. 1976 May;83(2):341–358. [PMC free article] [PubMed] [Google Scholar]

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