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. 1978 May;32(2):218–225.

Complement-fixing material in ther sera of patients with rheumatoid arthritis.

F C McDuffie, G G Hunder, R J Clark
PMCID: PMC1541285  PMID: 668197

Abstract

We investigated the properties of the complement-fixing material in samples of sera from fifteen patients with rheumatoid arthritis having low levels of C4, in order to determine its possible relationship to the immune complexes and rheumatoid factors. The complement-fixing material in most sera was of high molecular weight, 19s or greater, but in two sera it was also present in an intermediate zone (about 12s--15s). Cryoprecipitation did not remove the complement-fixing material except from serum with a elevated content of cryoglobulin. Analysis of the sera by analytical ultracentrifugation for the content of rheumatoid factor IgG complexes did not show a close relationship between the content of these complexes and the amount of complement-fixing material present in the sera. The responsible material could be absorbed by IgG-Sepharose together with the removal of rheumatoid factor. It was, however, possible to absorb the complement-fixing material from most sera with unconjugated Sepharose without significantly affecting the rheumatoid factor titres. Thus the complement-fixing material in the rheumatoid sera closely resembles an immune complex. It very likely contains rheumatoid factor, but it is not certain whether the material consists entirely of rheumatoid factors complexed to IgG or whether other antigen-antibody systems may also be present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agnello V., Winchester R. J., Kunkel H. G. Precipitin reactions of the C1q component of complement with aggregated gamma-globulin and immune complexes in gel diffusion. Immunology. 1970 Dec;19(6):909–919. [PMC free article] [PubMed] [Google Scholar]
  2. BEERS R. F., Jr, SIZER I. W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952 Mar;195(1):133–140. [PubMed] [Google Scholar]
  3. Baumal R., Broder I. Studies into the occurrence of soluble antigen-antibody complexes in disease. 3. Rheumatoid arthritis and other human diseases. Clin Exp Immunol. 1968 Jul;3(6):555–569. [PMC free article] [PubMed] [Google Scholar]
  4. Bokisch V. A., Müller-Eberhard H. J., Cochrane C. G. Isolation of a fragment (C3a) of the third component of human complement containing anaphylatoxin and chemotactic activity and description of an anaphylatoxin inactivator of human serum. J Exp Med. 1969 May 1;129(5):1109–1130. doi: 10.1084/jem.129.5.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CHODIRKER W. B., TOMASI T. B., Jr Low-molecular-weight rheumatoid factor. J Clin Invest. 1963 Jun;42:876–884. doi: 10.1172/JCI104780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Conn D. L., McDuffie F. C., Dyck P. J. Immunopathologic study of sural nerves in rheumatoid arthritis. Arthritis Rheum. 1972 Mar-Apr;15(2):135–143. doi: 10.1002/art.1780150202. [DOI] [PubMed] [Google Scholar]
  7. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  8. FRANKLIN E. C., HOLMAN H. R., MULLER-EBERHARD H. J., KUNKEL H. G. An unusual protein component of high molecular weight in the serum of certain patients with rheumatoid arthritis. J Exp Med. 1957 May 1;105(5):425–438. doi: 10.1084/jem.105.5.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hannestad K. Rheumatoid factors reacting with autologous native gamma-G-globulin and joint fluid gamma-G aggregates. Clin Exp Immunol. 1968 Sep;3(7):671–690. [PMC free article] [PubMed] [Google Scholar]
  10. Hunder G. G., McDuffie F. C., Huston K. A., Elveback L. R., Hepper N. G. Pleural fluid complement, complement conversion, and immune complexes in immunologic and nonimmunologic diseases. J Lab Clin Med. 1977 Dec;90(6):971–980. [PubMed] [Google Scholar]
  11. KULKA J. P., BOCKING D., ROPES M. W., BAUER W. Early joint lesions of rheumatoid arthritis; report of eight cases, with knee biopsies of lesions of less than one year's duration. AMA Arch Pathol. 1955 Feb;59(2):129–150. [PubMed] [Google Scholar]
  12. Kohler P. F., Müller-Eberhard H. J. Immunochemical quantitation of the third, fourth and fifth components of human complement: concentrations in the serum of healthy adults. J Immunol. 1967 Dec;99(6):1211–1216. [PubMed] [Google Scholar]
  13. Luthra H. S., McDuffie F. C., Hunder G. G., Samayoa E. A. Immune complexes in sera and synovial fluids of patients with rheumatoid arthritis. Radioimmunoassay with monocylonal rheumatoid factor. J Clin Invest. 1975 Aug;56(2):458–466. doi: 10.1172/JCI108112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  15. Marcus R. L., Townes A. S. The occurrence of cryoproteins in synovial fluid; the association of a complement-fixing activity in rheumatoid synovial fluid with cold-precipitable protein. J Clin Invest. 1971 Feb;50(2):282–293. doi: 10.1172/JCI106493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nelson R. A., Jr, Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum. Immunochemistry. 1966 Mar;3(2):111–135. doi: 10.1016/0019-2791(66)90292-8. [DOI] [PubMed] [Google Scholar]
  17. PLOTZ C. M., SINGER J. M. The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. Am J Med. 1956 Dec;21(6):888–892. [PubMed] [Google Scholar]
  18. Penttinen K., Wager O., Räsänen J. A., Myllylä G., Haapanen E. Platelet aggregation and cryo-IgM in the study of hepatitis and immune complex states. Clin Exp Immunol. 1973 Nov;15(3):409–416. [PMC free article] [PubMed] [Google Scholar]
  19. Pope R. M., Teller D. C., Mannik M. Intermediate complexes formed by self-association of IgG-rheumatoid factors. Ann N Y Acad Sci. 1975 Jun 13;256:82–87. doi: 10.1111/j.1749-6632.1975.tb36037.x. [DOI] [PubMed] [Google Scholar]
  20. SOKOLOFF L., MCCLUSKEY R. T., BUNIM J. J. Vascularity of the early subcutaneous nodule of rheumatoid arthritis. AMA Arch Pathol. 1953 Jun;55(6):475–495. [PubMed] [Google Scholar]
  21. Theofilopoulos A. N., Burtonboy G., LoSpalluto J. J., Ziff M. IgM rheumatoid factor and low molecular weight IgM. An association with vasculitis. Arthritis Rheum. 1974 May-Jun;17(3):272–284. doi: 10.1002/art.1780170312. [DOI] [PubMed] [Google Scholar]
  22. Theofilopoulos A. N., Wilson C. B., Dixon F. J. The Raji cell radioimmune assay for detecting immune complexes in human sera. J Clin Invest. 1976 Jan;57(1):169–182. doi: 10.1172/JCI108257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Torrigiani G., Ansell B. M., Chown E. E., Roitt I. M. Raised IgG antiglobulin factors in Still's disease. Ann Rheum Dis. 1969 Jul;28(4):424–427. doi: 10.1136/ard.28.4.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WALLER M. V., VAUGHAN J. H. Use of anti-Rh sera for demonstrating agglutination activating factor in rheumatoid arthritis. Proc Soc Exp Biol Med. 1956 May;92(1):198–200. doi: 10.3181/00379727-92-22426. [DOI] [PubMed] [Google Scholar]
  25. Ward P. A. A plasmin-split fragment of C'3 as a new chemotactic factor. J Exp Med. 1967 Aug 1;126(2):189–206. doi: 10.1084/jem.126.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weisman M., Zvaifler N. Cryoimmunoglobulinemia in rheumatoid arthritis. Significance in serum of patients with rheumatoid vasculitis. J Clin Invest. 1975 Sep;56(3):725–739. doi: 10.1172/JCI108144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Winchester R. J., Agnello V., Kunkel H. G. Gamma globulin complexes in synovial fluids of patients with rheumatoid arthritis. Partial characterization and relationship to lowered complement levels. Clin Exp Immunol. 1970 May;6(5):689–706. [PMC free article] [PubMed] [Google Scholar]

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