Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1976 Aug;25(2):185–190.

Hidden IgG antiglobulins in normal human serum.

F C Hay, L J Nineham, G Torrigiani, I M Roitt
PMCID: PMC1541363  PMID: 1085235

Abstract

Hidden antiglobulins reacting with whole rabbit immunoglobulin were found by radioassay or indirect haemagglutination after gel filtration or ultracentrifugation of normal serum. Suprisingly, both the IgM and IgG antiglobulins were present in the same macroglobulin fractions. The IgG antiglobulins could be dissociated into 7S components by separating the serum under acid conditions. Antiglobulin activity was a function of the Fab region.

Full text

PDF
187

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen J. C., Kunkel H. G. Hidden rheumatoid factors with specificity for native gamma globulins. Arthritis Rheum. 1966 Dec;9(6):758–768. doi: 10.1002/art.1780090603. [DOI] [PubMed] [Google Scholar]
  2. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  3. Hannestad K. Presence of aggregated gamma-G-globulin in certain rheumatoid synovial effusions. Clin Exp Immunol. 1967 Jul;2(4):511–529. [PMC free article] [PubMed] [Google Scholar]
  4. Hay F. C., Nineham L. J., Roitt I. M. Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis. Br Med J. 1975 Jul 26;3(5977):203–204. doi: 10.1136/bmj.3.5977.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KUNKEL H. G., MULLER-EBERHARD H. J., FUDENBERG H. H., TOMASI T. B. Gamma globulin complexes in rheumatoid arthritis and certain other conditions. J Clin Invest. 1961 Jan;40:117–129. doi: 10.1172/JCI104224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  7. OSTERLAND C. K., HARBOE M., KUNKEL H. G. Anti-gamma-globulin factors in human sera revealed by enzymatic splitting of anti-Rh antibodies. Vox Sang. 1963 Mar-Apr;8:133–152. doi: 10.1111/j.1423-0410.1963.tb03290.x. [DOI] [PubMed] [Google Scholar]
  8. Pope R. M., Teller D. C., Mannik M. The molecular basis of self-association of antibodies to IgG (rheumatoid factors) in rheumatoid arthritis. Proc Natl Acad Sci U S A. 1974 Feb;71(2):517–521. doi: 10.1073/pnas.71.2.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. TORRIGIANI G., ROITT I. M. THE ENHANCEMENT OF 19S ANTIBODY PRODUCTION BY PARTICULATE ANTIGEN. J Exp Med. 1965 Jul 1;122:181–193. doi: 10.1084/jem.122.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Torrigiani G., Ansell B. M., Chown E. E., Roitt I. M. Raised IgG antiglobulin factors in Still's disease. Ann Rheum Dis. 1969 Jul;28(4):424–427. doi: 10.1136/ard.28.4.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Torrigiani G., Roitt I. M. Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes. Ann Rheum Dis. 1967 Jul;26(4):334–340. doi: 10.1136/ard.26.4.334. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES