Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1976 Sep;25(3):428–436.

Intracytoplasmic and surface-bound immunoglobulins in "nonsecretory" and Bence-Jones myeloma.

J L Preud'Homme, D Hurez, F Danon, J C Brouet, M Seligmann
PMCID: PMC1541419  PMID: 822974

Abstract

Immunoglobulins were studied at the cellular level by direct immunofluorescence in twenty-five patients with 'nonsecretory' myeloma and thirty-six patiens with Bence-Jones (BJ) myeloma. The results were compared with those obtained in a control group of thirty-six patients with common secretory myeloma. A monoclonal Ig (IgG in eighteen, IgA in three and kappa chains only in three cases) was found in the cytoplasm of the plasma cells from all the patients with 'nonsecretory' myeloma, with a striking dysbalance in the staining brightness for the heavy and the light chains. A similar dysbalance in staining was also observed for plasma cell surface Ig chains but in the opposite way. In twenty patients with BJ myeloma studied for cytoplasmic Ig only, determinants of a heavy chain were clearly found in four cases. When surface Ig were studied also, the production of gamma chains in addition to the light chain could be ascertained in six of sixteen cases. In addition, IgM with the same light chain type as the BJ protein was detected at the cell surface on plasma cells and lymphocytes in two of these sixteen patients. 'Monoclonal' populations of B lymphocytes bearing the same Ig chains as those produced by the myeloma cells were detectable in five of eleven 'nonsecretory' myeloma and in five of sixteen BJ myeloma patients. Normal blood B lymphocytes were in decreased number, particularly when a 'monoclonal' lymphocytic population was detected. Data are discussed which suggest that plasma cells from most patients with 'nonsecretory' myeloma might synthesize and secrete Ig molecules with structurally abnormal chains that are then quickly degraded.

Full text

PDF
430

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arend W. P., Adamson J. W. Nonsecretory myeloma. Immunofluorescent demonstration of paraprotein within bone marrow plasma cells. Cancer. 1974 Mar;33(3):721–728. doi: 10.1002/1097-0142(197403)33:3<721::aid-cncr2820330317>3.0.co;2-d. [DOI] [PubMed] [Google Scholar]
  2. Azar H. A., Zaino E. C., Pham T. D., Yannopoulos K. "Nonsecretory" plasma cell myeloma: observations on seven cases with electron microscopic studies. Am J Clin Pathol. 1972 Dec;58(6):618–629. doi: 10.1093/ajcp/58.6.618. [DOI] [PubMed] [Google Scholar]
  3. Baumal R., Birshtein B. K., Coffino P., Scharff M. D. Mutations in immunoglobulin-producing mouse myeloma cells. Science. 1973 Oct 12;182(4108):164–166. doi: 10.1126/science.182.4108.164. [DOI] [PubMed] [Google Scholar]
  4. Bedou G., Le Goff P., Besson G., Blanguernon P., Garré H. Myélome non excrétant révélé par une compression médullaire. Sem Hop. 1974 Oct 8;50(40):2477–2480. [PubMed] [Google Scholar]
  5. Birshtein B. K., Preud'homme J. L., Scharff M. D. Variants of mouse myeloma cells that produce short immunoglobulin heavy chains. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3478–3482. doi: 10.1073/pnas.71.9.3478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Broder S., Humphrey R., Durm M., Blackman M., Meade B., Goldman C., Strober W., Waldmann T. Impaired synthesis of polyclonal (non-paraprotein) immunoglobulins by circulating lymphocytes from patients with multiple myeloma Role of suppressor cells. N Engl J Med. 1975 Oct 30;293(18):887–892. doi: 10.1056/NEJM197510302931801. [DOI] [PubMed] [Google Scholar]
  7. Carbone P. P., Kellerhouse L. E., Gehan E. A. Plasmacytic myeloma. A study of the relationship of survival to various clinical manifestations and anomalous protein type in 112 patients. Am J Med. 1967 Jun;42(6):937–948. doi: 10.1016/0002-9343(67)90074-5. [DOI] [PubMed] [Google Scholar]
  8. Chen Y., Bhoopalam N., Yakulis V., Heller P. Changes in lymphocyte surface immunoglobulins in myeloma and the effect of an RNA-containing plasma factor. Ann Intern Med. 1975 Nov;83(5):625–631. doi: 10.7326/0003-4819-83-5-625. [DOI] [PubMed] [Google Scholar]
  9. Coffino P., Scharff M. D. Rate of somatic mutation in immunoglobulin production by mouse myeloma cells. Proc Natl Acad Sci U S A. 1971 Jan;68(1):219–223. doi: 10.1073/pnas.68.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cowan N. J., Secher D. S., Milstein C. Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA. J Mol Biol. 1974 Dec 25;90(4):691–701. doi: 10.1016/0022-2836(74)90533-6. [DOI] [PubMed] [Google Scholar]
  11. Gach J., Simar L., Salmon J. Multiple myeloma without M-type proteinemia. Report of a case with immunologic and ultrastructure studies. Am J Med. 1971 Jun;50(6):835–844. doi: 10.1016/0002-9343(71)90193-8. [DOI] [PubMed] [Google Scholar]
  12. Giacomoni D., Yakulis V., Wang S. R., Cooke A., Dray S., Heller P. In vitro conversion of normal mouse lymphocytes by plasmacytoma RNA to express idiotypic specificities on their surface characteristic of the plasmacytoma immunoglobulin. Cell Immunol. 1974 Mar 30;11(1-3):389–400. doi: 10.1016/0008-8749(74)90037-9. [DOI] [PubMed] [Google Scholar]
  13. Hobbs J. R. Immunochemical classes of myelomatosis. Including data from a therapeutic trial conducted by a Medical Research Council working party. Br J Haematol. 1969 Jun;16(6):599–606. doi: 10.1111/j.1365-2141.1969.tb00440.x. [DOI] [PubMed] [Google Scholar]
  14. Hobbs J. R. Modes of escape from therapeutic control in myelomatosis. Br Med J. 1971 May 8;2(5757):325–325. doi: 10.1136/bmj.2.5757.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hurez D., Preud'Homme J. L., Seligmann M. Intracellular "monoclonal" immunoglobulin in non-secretory human myeloma. J Immunol. 1970 Jan;104(1):263–264. [PubMed] [Google Scholar]
  16. Knapp W., Schuit H. R., Bolhuis R. L., Hijmans W. Surface immunoglobulins in chronic lymphatic leukaemia, macroglobulinaemia and myelomatosis. Clin Exp Immunol. 1974 Apr;16(4):541–552. [PMC free article] [PubMed] [Google Scholar]
  17. Lindström F. D., Hardy W. R., Eberle B. J., Williams R. C., Jr Multiple myeloma and benign monoclonal gammopathy: differentiation by immunofluorescence of lymphocytes. Ann Intern Med. 1973 Jun;78(6):837–844. doi: 10.7326/0003-4819-78-6-837. [DOI] [PubMed] [Google Scholar]
  18. Mellstedt H., Hammarström S., Holm G. Monoclonal lymphocyte population in human plasma cell myeloma. Clin Exp Immunol. 1974 Jul;17(3):371–384. [PMC free article] [PubMed] [Google Scholar]
  19. Menkes C. J., Herreman G., Preud'homme J. L., Godeau P., Delbarre F. Myélome à plasmocytes non excrétants (à propos de deux nouvelles observations. Nouv Presse Med. 1972 Jan 29;1(5):309–312. [PubMed] [Google Scholar]
  20. Morrison S. L., Scharff M. D. Heavy chain-producing variants of a mouse myeloma cell line. J Immunol. 1975 Feb;114(2 Pt 1):655–659. [PubMed] [Google Scholar]
  21. Pernis B., Forni L., Amante L. Immunoglobulins as cell receptors. Ann N Y Acad Sci. 1971 Dec 31;190:420–431. doi: 10.1111/j.1749-6632.1971.tb13552.x. [DOI] [PubMed] [Google Scholar]
  22. Prend'homme J. L., Buxbaum J., Scharff M. D. Mutagenesis of mouse myeloma cells with 'Melphalan'. Nature. 1973 Oct 12;245(5424):320–322. doi: 10.1038/245320a0. [DOI] [PubMed] [Google Scholar]
  23. Preud'homme J. L., Labaume S. Immunofluorescent staining of human lymphocytes for the detection of surface immunoglobulins. Ann N Y Acad Sci. 1975 Jun 30;254:254–261. doi: 10.1111/j.1749-6632.1975.tb29175.x. [DOI] [PubMed] [Google Scholar]
  24. Preud'homme J. L., Seligmann M. Immunoglobulins on the surface of lymphoid cells in Waldenström's macroglobulinemia. J Clin Invest. 1972 Mar;51(3):701–705. doi: 10.1172/JCI106858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Preud'homme J. L., Seligmann M. Surface immunoglobulins on human lymphoid cells. Prog Clin Immunol. 1974;2:121–174. [PubMed] [Google Scholar]
  26. Prost A., Soulilou J. P., Gardais J., Hurez D., Cottin S. Deux nouvelles observations de myélomes á plasmocytes d'emblée ou secondairement non-excrétants. Rev Rhum Mal Osteoartic. 1973 Feb;40(2):151–157. [PubMed] [Google Scholar]
  27. River G. L., Tewksbury D. A., Fudenberg H. H. "Nonsecretory" multiple myeloma. Blood. 1972 Aug;40(2):204–206. [PubMed] [Google Scholar]
  28. Stein H., Kaiserling E. Myeloma producing nonsecretory IgM and secretory IgG. Scand J Haematol. 1974;12(4):274–283. doi: 10.1111/j.1600-0609.1974.tb00210.x. [DOI] [PubMed] [Google Scholar]
  29. Taylor C. R., Mason D. Y. The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique. Clin Exp Immunol. 1974 Nov;18(3):417–429. [PMC free article] [PubMed] [Google Scholar]
  30. Uhr J. W., Vitetta E. S. Synthesis, biochemistry and dynamics of cell surface immunoglobulin on lymphocytes. Fed Proc. 1973 Jan;32(1):35–40. [PubMed] [Google Scholar]
  31. Whicher J. T., Davies J. D., Grayburn J. A. Intact and fragmented intracellular immunoglobulin in a case of non-secretory myeloma. J Clin Pathol. 1975 Jan;28(1):54–59. doi: 10.1136/jcp.28.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Zolla S., Buxbaum J., Franklin E. C., Scharff M. D. Synthesis and assembly of immunoglobulins by malignant human plasmacytes. I. Myelomas producing gamma-chains and light chains. J Exp Med. 1970 Jul 1;132(1):148–162. doi: 10.1084/jem.132.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Zolla S., Naor D., Tanapatchaiyapong P. Cellular basis of immunodepression in mice with plasmacytomas. J Immunol. 1974 Jun;112(6):2068–2076. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES