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. 1988 Jul;73(1):128–133.

Selective cytotoxicity of an oxygen-radical-generating enzyme conjugated to a monoclonal antibody.

M G Battelli 1, A Abbondanza 1, P L Tazzari 1, A Dinota 1, S Rizzi 1, G Grassi 1, M Gobbi 1, F Stirpe 1
PMCID: PMC1541466  PMID: 3262464

Abstract

The monoclonal antibody 8A, which recognizes a human plasma cell-associated antigen, was covalently linked to xanthine oxidase in a conjugate maintaining both immunological and enzymatic properties. A significant degree of target cell lysis was obtained at an enzyme concentration that was ineffective on non-target cells and on myeloid staminal cells (CFU-GM). The cytotoxic activity was abolished by an excess of antibody, by allopurinol and by superoxide dismutase and catalase. A possible use of the conjugate for bone marrow purging in multiple myeloma patients is suggested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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