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. 1988 Jul;73(1):149–154.

IgD in nasopharyngeal secretions and tonsils from otitis-prone children.

C H Sørensen 1, P L Larsen 1
PMCID: PMC1541482  PMID: 3048811

Abstract

Quantification of IgD was performed by ELISA in 180 plasma samples and 83 nasopharyngeal secretions (NPS) from children aged 2-162 months with varying degrees of recurrent otitis media. Furthermore, in 24 of the children the density of the IgD-immunocytes (IgD-cells) was calculated in immunoenzyme-stained cross-sections of their nasopharyngeal tonsils (NPT). Owing to a considerable variation of the IgD cell density throughout the NPT, a semi-quantitative counting system was applied. An irregular distribution of plasma IgD was observed during childhood and maximum levels were found between 48 and 72 months of age. However, an even distribution of plasma IgD was found among the four groups of children investigated. Based on calculations of the transudation of albumin from plasma to the NPS the amount of locally produced IgD in NPS (NPS-IgD (local] was estimated to 88% (range 41-99%). Significantly higher levels of NPS-IgD (local) were found in otitis-prone children than in the other groups. Moreover, a positive correlation was calculated between levels of NPS-IgD (local) and NPT-IgD cell density, indicating that NPT, being the local lymphoepithelial tissue, also functions as an important source of NPS-IgD. NPS-IgD was not found to be associated with secretory component, indicating a passive transfer of IgD through the mucosal membranes. Our results support the hypothesis of an association between the occurrence of IgD in the mucosa and secretions of the upper respiratory tract with localized inflammatory events.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black S. J., van der Loo W., Loken M. R., Herzenberg L. A. Expression of IgD by murine lymphocytes. Loss of surface IgD indicates maturation of memory B cells. J Exp Med. 1978 Apr 1;147(4):984–996. doi: 10.1084/jem.147.4.984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brandtzaeg P., Gjeruldsen S. T., Korsrud F., Baklien K., Berdal P., Ek J. The human secretory immune system shows striking heterogeneity with regard to involvement of J chain-positive IgD immunocytes. J Immunol. 1979 Feb;122(2):503–510. [PubMed] [Google Scholar]
  3. Brandtzaeg P. Immunohistochemical characterization of intracellular J-chain and binding site for secretory component (SC) in human immunoglobulin (Ig)-producing cells. Mol Immunol. 1983 Sep;20(9):941–966. doi: 10.1016/0161-5890(83)90036-6. [DOI] [PubMed] [Google Scholar]
  4. Brandtzaeg P., Karlsson G., Hansson G., Petruson B., Björkander J., Hanson L. A. The clinical condition of IgA-deficient patients is related to the proportion of IgD- and IgM-producing cells in their nasal mucosa. Clin Exp Immunol. 1987 Mar;67(3):626–636. [PMC free article] [PubMed] [Google Scholar]
  5. Brandtzaeg P. Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique. Immunology. 1974 Jun;26(6):1101–1114. [PMC free article] [PubMed] [Google Scholar]
  6. Brandtzaeg P., Surjan L., Jr, Berdal P. Immunoglobulin systems of human tonsils. I. Control subjects of various ages: quantification of Ig-producing cells, tonsillar morphometry and serum Ig concentrations. Clin Exp Immunol. 1978 Mar;31(3):367–381. [PMC free article] [PubMed] [Google Scholar]
  7. Brandtzaeg P. The secretory immune system of lactating human mammary glands compared with other exocrine organs. Ann N Y Acad Sci. 1983 Jun 30;409:353–382. doi: 10.1111/j.1749-6632.1983.tb26883.x. [DOI] [PubMed] [Google Scholar]
  8. Clausen P. P., Jacobsen M., Johansen P., Thommesen N. Immunohistochemical demonstration of intracellular immunoglobulin in formalin fixed, paraffin embedded sections, as staining method in diagnostic work. Acta Pathol Microbiol Scand C. 1979 Aug;87C(4):307–312. [PubMed] [Google Scholar]
  9. Cooper M. D., Kuritani T., Chen C., Lehmeyer J. E., Gathings W. E. Expression of IgD as a function of B-cell differentiation. Ann N Y Acad Sci. 1982;399:146–156. doi: 10.1111/j.1749-6632.1982.tb25670.x. [DOI] [PubMed] [Google Scholar]
  10. Donovan R., Johansson S. G., Bennich H., Soothill J. F. Immunoglobulins in nasal polyp fluid. Int Arch Allergy Appl Immunol. 1970;37(2):154–166. doi: 10.1159/000230229. [DOI] [PubMed] [Google Scholar]
  11. Dunnette S. L., Gleich G. J., Miller R. D., Kyle R. A. Measurement of IgD by a double antibody radioimmunoassay: demonstration of an apparent trimodal distribution of IgD levels in normal human sera. J Immunol. 1977 Nov;119(5):1727–1731. [PubMed] [Google Scholar]
  12. Jelinek D. F., Lipsky P. E. Regulation of human B lymphocyte activation, proliferation, and differentiation. Adv Immunol. 1987;40:1–59. doi: 10.1016/s0065-2776(08)60237-0. [DOI] [PubMed] [Google Scholar]
  13. Johansson S. G., Berg T. Immunoglobulin levels in healthy children. Acta Paediatr Scand. 1967 Nov;56(6):572–579. doi: 10.1111/j.1651-2227.1967.tb15982.x. [DOI] [PubMed] [Google Scholar]
  14. Kett K., Brandtzaeg P., Radl J., Haaijman J. J. Different subclass distribution of IgA-producing cells in human lymphoid organs and various secretory tissues. J Immunol. 1986 May 15;136(10):3631–3635. [PubMed] [Google Scholar]
  15. Korsrud F. R., Brandtzaeg P. Immune systems of human nasopharyngeal and palatine tonsils: histomorphometry of lymphoid components and quantification of immunoglobulin-producing cells in health and disease. Clin Exp Immunol. 1980 Feb;39(2):361–370. [PMC free article] [PubMed] [Google Scholar]
  16. Korsrud F. R., Brandtzaeg P. Influence of tonsillar disease on the expression of J chain by immunoglobulin-producing cells in human palatine and nasopharyngeal tonsils. Scand J Immunol. 1981;13(3):281–287. doi: 10.1111/j.1365-3083.1981.tb00136.x. [DOI] [PubMed] [Google Scholar]
  17. Korsrud F. R., Brandtzaeg P. Quantitative immunohistochemistry of immunoglobulin- and J-chain-producing cells in human parotid and submandibular salivary glands. Immunology. 1980 Feb;39(2):129–140. [PMC free article] [PubMed] [Google Scholar]
  18. Kuritani T., Cooper M. D. Human b-cell differentiation. I. Analysis of immunoglobulin heavy chain switching using monoclonal anti-immunoglobulin M, G, and A antibodies and pokeweed mitogen-induced plasma cell differentiation. J Exp Med. 1982 Mar 1;155(3):839–851. doi: 10.1084/jem.155.3.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Long S. S., Henretig F. M., Teter M. J., McGowan K. L. Nasopharyngeal flora and acute otitis media. Infect Immun. 1983 Sep;41(3):987–991. doi: 10.1128/iai.41.3.987-991.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Plebani A., Mira E., Mevio E., Monafo V., Notarangelo L. D., Avanzini A., Ugazio A. G. IgM and IgD concentrations in the serum and secretions of children with selective IgA deficiency. Clin Exp Immunol. 1983 Sep;53(3):689–696. [PMC free article] [PubMed] [Google Scholar]
  21. Rabbitts T. H., Forster A., Milstein C. P. Human immunoglobulin heavy chain genes: evolutionary comparisons of C mu, C delta and C gamma genes and associated switch sequences. Nucleic Acids Res. 1981 Sep 25;9(18):4509–4524. doi: 10.1093/nar/9.18.4509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rowe D. S., Crabbé P. A., Turner M. W. Immunoglobulin D in serum, body fluids and lymphoid tissues. Clin Exp Immunol. 1968 Jul;3(6):477–490. [PMC free article] [PubMed] [Google Scholar]
  23. Surjan L., Jr, Brandtzaeg P., Berdal P. Immunoglobulin systems of human tonsils. II. Patients with chronic tonsillitis or tonsillar hyperplasia: quantification of Ig-producing cells, tonsillar morphometry and serum Ig concentrations. Clin Exp Immunol. 1978 Mar;31(3):382–390. [PMC free article] [PubMed] [Google Scholar]
  24. Sørensen C. H., Nielsen L. K. Nasopharyngeal secretory immunoglobulins in children with recurrent acute otitis media and secretory otitis media. APMIS. 1988 Mar;96(3):199–205. doi: 10.1111/j.1699-0463.1988.tb05291.x. [DOI] [PubMed] [Google Scholar]
  25. Sørensen C. H. Quantitative aspects of IgD and secretory immunoglobulins in middle ear effusions. Int J Pediatr Otorhinolaryngol. 1983 Dec;6(3):247–253. doi: 10.1016/s0165-5876(83)80126-8. [DOI] [PubMed] [Google Scholar]
  26. Sørensen C. H. Secretory IgA enzyme immunoassay. Application of a model for computation of the standard curve. Scand J Clin Lab Invest. 1982 Nov;42(7):577–583. doi: 10.1080/00365518209168133. [DOI] [PubMed] [Google Scholar]

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