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. 1988 Mar;71(3):428–432.

Enhancement of DTH reaction and inhibition of the expression of class II transplantation antigens by in vivo treatment with antibodies against gamma-interferon.

C Skoglund 1, A Scheynius 1, R Holmdahl 1, P H Van der Meide 1
PMCID: PMC1541681  PMID: 2454769

Abstract

During the delayed type of hypersensitivity (DTH) reaction in the skin, class II transplantation antigens are expressed on the keratinocytes. This induction is attributed to the action of gamma-interferon (IFN-gamma). We have now studied the influence of antibodies against IFN-gamma on the DTH-reaction. Lewis rats were sensitized to 2,4-dinitro-1-fluorobenzene (DNFB) and challenged 5 days later with DNFB on the ears. Immediately before challenge each animal in one group (n = 16) was given 1 mg of mouse monoclonal antibodies (MoAb) against rats IFN-gamma, denoted DB-1, intraperitoneally (i.p.), another group (n = 15), 1 mg of an irrelevant MoAb and a third group (n = 11) was left untreated. The ear thickness was measured with a micrometer, before challenge and after 24, 48 and 72 h. At 72 h all rats were killed, the ears cut off, snap frozen and stained with immunoperoxidase using MoAbs OX 6 and OX 17, directed against rat class II antigens. The DB-1 treated group was found to have a larger ear swelling that was statistically significant at each time point compared with the other two groups. Furthermore, the animals given DB-1 showed class II antigen expression on Langerhans' cells, but almost none on keratinocytes. In contrast, the rats in the two other groups displayed a moderate to strong expression of class II antigens on keratinocytes as well as on Langerhans' cells. It is concluded that DB-1 can inhibit class II antigen expression on keratinocytes during the DTH- reaction and also enhance the local response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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