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. 1989 Apr;76(1):117–120.

Spectrotypic analysis of humoral response in human hydatidosis.

R D'Amelio 1, G Del Giudice 1, F De Rosa 1, G Brighouse 1, A Teggi 1, P H Lambert 1
PMCID: PMC1541721  PMID: 2786776

Abstract

The spectrotypic patterns of anti-Echinococcus granulosus and anti-F(ab')2 antibodies were analysed in 13 patients with different forms of hydatidosis by means of isoelectric focusing (IEF). Specific anti-echinococcus antibodies and specific echinococcus antigens were obtained by crossed affinity chromatography between patient serum and cyst fluid, as previously described (D'Amelio et al., 1985). A wide spectrotype in the pattern of anti-Echinococcus antibodies was observed, with strong homologies among the different sera mainly at acid and neutral pH. One serum displayed faint, nearly inapparent bands. Conversely, the pattern of anti-F(ab')2 antibodies in the six patients in whom they were positive was limited but heterogeneous, ranging from monoclonal through oligoclonal to polyclonal. A predominant clone was identified in the patient with a polyclonal pattern. A correlation between qualitative IEF spectrotypic patterns and the quantitative results of immunoenzymatic assays was observed for both types of antibodies.

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Selected References

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