Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1987 Oct;70(1):182–191.

Expression of functional interleukin 2 receptors on chronic lymphocytic leukaemia B lymphocytes is modulated by recombinant interleukin 2.

J J Murphy 1, V Malkovska 1, L Hudson 1, R E Millard 1
PMCID: PMC1542198  PMID: 3121222

Abstract

In 16 of 18 chronic lymphocytic leukaemia (CLL) patients examined, a significant proportion of B cells in the leukaemic clone bound monoclonal antibodies specific for the interleukin 2 (IL-2) receptor site (CD25). B lymphocytes from patients tested showed a direct response to recombinant interleukin (rIL-2) during culture in vitro as shown by: (a) a ligand-mediated upregulation in the level of IL-2 receptor (IL-2R) expression (12 of 12 patients), (b) an increase in cell size (eight of nine patients), (c) an increase in 3H-thymidine uptake (four of six patients). Taken together, this evidence suggests that the majority of leukaemic B cells from all the CLL patients examined expressed functional IL-2 receptors in vitro. Intriguingly, maximal receptor upregulation or increase in cell size was achieved at a lower concentration (50 u/ml) of rIL-2 than was required to achieve maximal 3H-thymidine incorporation.

Full text

PDF
182

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armitage R. J., Cawley J. C. Normal and certain leukaemic B cells express IL-2 receptors without in vitro activation. Clin Exp Immunol. 1986 Feb;63(2):298–302. [PMC free article] [PubMed] [Google Scholar]
  2. Fu S. M., Chiorazzi N., Kunkel H. G., Halper J. P., Harris S. R. Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes. J Exp Med. 1978 Dec 1;148(6):1570–1578. doi: 10.1084/jem.148.6.1570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Godal T., Funderud S. Human B-cell neoplasms in relation to normal B-cell differentiation and maturation processes. Adv Cancer Res. 1982;36:211–255. doi: 10.1016/s0065-230x(08)60426-1. [DOI] [PubMed] [Google Scholar]
  4. Gordon J., Mellstedt H., Aman P., Biberfeld P., Björkholm M., Klein G. Phenotypes in chronic B-lymphocytic leukemia probed by monoclonal antibodies and immunoglobulin secretion studies: identification of stages of maturation arrest and the relation to clinical findings. Blood. 1983 Oct;62(4):910–917. [PubMed] [Google Scholar]
  5. Guglielmi P., Preud'homme J. L., Ciorbaru-Barot R., Seligmann M. Mitogen-induced maturation of chronic lymphocytic leukemia B lymphocytes. J Clin Immunol. 1982 Jul;2(3):186–196. doi: 10.1007/BF00915221. [DOI] [PubMed] [Google Scholar]
  6. Jelinek D. F., Splawski J. B., Lipsky P. E. The roles of interleukin 2 and interferon-gamma in human B cell activation, growth and differentiation. Eur J Immunol. 1986 Aug;16(8):925–932. doi: 10.1002/eji.1830160809. [DOI] [PubMed] [Google Scholar]
  7. Korsmeyer S. J., Greene W. C., Cossman J., Hsu S. M., Jensen J. P., Neckers L. M., Marshall S. L., Bakhshi A., Depper J. M., Leonard W. J. Rearrangement and expression of immunoglobulin genes and expression of Tac antigen in hairy cell leukemia. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4522–4526. doi: 10.1073/pnas.80.14.4522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lantz O., Grillot-Courvalin C., Schmitt C., Fermand J. P., Brouet J. C. Interleukin 2-induced proliferation of leukemic human B cells. J Exp Med. 1985 May 1;161(5):1225–1230. doi: 10.1084/jem.161.5.1225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Liang S. M., Allet B., Rose K., Hirschi M., Liang C. M., Thatcher D. R. Characterization of human interleukin 2 derived from Escherichia coli. Biochem J. 1985 Jul 15;229(2):429–439. doi: 10.1042/bj2290429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Malkovska V., Murphy J., Hudson L., Bevan D. Direct effect of interleukin 2 on chronic lymphocytic leukaemia B cell functions and morphology. Clin Exp Immunol. 1987 Jun;68(3):677–684. [PMC free article] [PubMed] [Google Scholar]
  11. Muraguchi A., Kehrl J. H., Longo D. L., Volkman D. J., Smith K. A., Fauci A. S. Interleukin 2 receptors on human B cells. Implications for the role of interleukin 2 in human B cell function. J Exp Med. 1985 Jan 1;161(1):181–197. doi: 10.1084/jem.161.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Preud'homme J. L., Seligmann M. Surface bound immunoglobulins as a cell marker in human lymphoproliferative diseases. Blood. 1972 Dec;40(6):777–794. [PubMed] [Google Scholar]
  13. Rai K. R., Sawitsky A., Cronkite E. P., Chanana A. D., Levy R. N., Pasternack B. S. Clinical staging of chronic lymphocytic leukemia. Blood. 1975 Aug;46(2):219–234. [PubMed] [Google Scholar]
  14. Romagnani S., Del Prete G., Giudizi M. G., Biagiotti R., Almerigogna F., Tiri A., Alessi A., Mazzetti M., Ricci M. Direct induction of human B-cell differentiation by recombinant interleukin-2. Immunology. 1986 May;58(1):31–35. [PMC free article] [PubMed] [Google Scholar]
  15. Romagnani S., Giudizi G. M., Almerigogna F., Biagiotti R., Alessi A., Mingari C., Liang C. M., Moretta L., Ricci M. Analysis of the role of interferon-gamma, interleukin 2 and a third factor distinct from interferon-gamma and interleukin 2 in human B cell proliferation. Evidence that they act at different times after B cell activation. Eur J Immunol. 1986 Jun;16(6):623–629. doi: 10.1002/eji.1830160607. [DOI] [PubMed] [Google Scholar]
  16. Saxon A., Feldhaus J., Robins R. A. Single step separation of human T and B cells using AET treated srbc rosettes. J Immunol Methods. 1976;12(3-4):285–288. doi: 10.1016/0022-1759(76)90050-8. [DOI] [PubMed] [Google Scholar]
  17. Smith K. A., Cantrell D. A. Interleukin 2 regulates its own receptors. Proc Natl Acad Sci U S A. 1985 Feb;82(3):864–868. doi: 10.1073/pnas.82.3.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stashenko P., Nadler L. M., Hardy R., Schlossman S. F. Characterization of a human B lymphocyte-specific antigen. J Immunol. 1980 Oct;125(4):1678–1685. [PubMed] [Google Scholar]
  19. Teshigawara K., Wang H. M., Kato K., Smith K. A. Interleukin 2 high-affinity receptor expression requires two distinct binding proteins. J Exp Med. 1987 Jan 1;165(1):223–238. doi: 10.1084/jem.165.1.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thorley-Lawson D. A., Schooley R. T., Bhan A. K., Nadler L. M. Epstein-Barr virus superinduces a new human B cell differentiation antigen (B-LAST 1) expressed on transformed lymphoblasts. Cell. 1982 Sep;30(2):415–425. doi: 10.1016/0092-8674(82)90239-2. [DOI] [PubMed] [Google Scholar]
  21. Tsudo M., Uchiyama T., Uchino H. Expression of Tac antigen on activated normal human B cells. J Exp Med. 1984 Aug 1;160(2):612–617. doi: 10.1084/jem.160.2.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tötterman T. H., Nilsson K., Sundström C. Phorbol ester-induced differentiation of chronic lymphocytic leukaemia cells. Nature. 1980 Nov 13;288(5787):176–178. doi: 10.1038/288176a0. [DOI] [PubMed] [Google Scholar]
  23. Waldmann T. A., Goldman C. K., Leonard W. J., Depper J. M., Robb R. J., Korsmeyer S. J., Greene W. C. Interleukin-2 receptors on activated malignant and normal B-cells. Curr Top Microbiol Immunol. 1984;113:96–101. doi: 10.1007/978-3-642-69860-6_18. [DOI] [PubMed] [Google Scholar]
  24. Yoshizaki K., Nakagawa T., Kaieda T., Muraguchi A., Yamamura Y., Kishimoto T. Induction of proliferation and Ig production in human B leukemic cells by anti-immunoglobulins and T cell factors. J Immunol. 1982 Mar;128(3):1296–1301. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES