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. 1987 Aug;69(2):255–262.

BCG induced CD4+ cytotoxic T cells from BCG vaccinated healthy subjects: relation between cytotoxicity and suppression in vitro.

A S Mustafa 1, T Godal 1
PMCID: PMC1542411  PMID: 2958195

Abstract

Mycobacterial antigen specific cytotoxic T cells killing antigen-pulsed antigen presenting cells (APC) were induced from peripheral blood mononuclear cells (PBMC) of BCG-vaccinated healthy subjects after activation in vitro with BCG. CD8+ depleted cells were as effective as PBMC, indicating that CD4+ cells play a dominant role in this phenomenon. CD4+ T cell clones raised against BCG also exhibited mycobacterial antigen specific cytotoxicity and suppressed BCG-driven selfproliferation. However, the same clones could either suppress or enhance the proliferation of other T cell clones from the same subject. The possible function in vivo of the cytotoxicity mediated by CD4+ T cells is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrew M. E., Churilla A. M., Malek T. R., Braciale V. L., Braciale T. J. Activation of virus specific CTL clones: antigen-dependent regulation of interleukin 2 receptor expression. J Immunol. 1985 Feb;134(2):920–925. [PubMed] [Google Scholar]
  2. Bird A. G., McLachlan S. M., Britton S. Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein-Barr virus-induced B-cell lines. Nature. 1981 Jan 22;289(5795):300–301. doi: 10.1038/289300a0. [DOI] [PubMed] [Google Scholar]
  3. Cantrell D. A., Smith K. A. The interleukin-2 T-cell system: a new cell growth model. Science. 1984 Jun 22;224(4655):1312–1316. doi: 10.1126/science.6427923. [DOI] [PubMed] [Google Scholar]
  4. Champion B. R., Hutchings P., Davies S., Marshall-Clarke S., Cooke A., Roitt I. M. Helper and suppressor activities of an autoreactive mouse thyroglobulin-specific T-cell clone. Immunology. 1986 May;58(1):51–56. [PMC free article] [PubMed] [Google Scholar]
  5. Chiplunkar S., De Libero G., Kaufmann S. H. Mycobacterium leprae-specific Lyt-2+ T lymphocytes with cytolytic activity. Infect Immun. 1986 Dec;54(3):793–797. doi: 10.1128/iai.54.3.793-797.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. De Libero G., Kaufmann S. H. Antigen-specific Lyt-2+ cytolytic T lymphocytes from mice infected with the intracellular bacterium Listeria monocytogenes. J Immunol. 1986 Oct 15;137(8):2688–2694. [PubMed] [Google Scholar]
  7. Kaplan D. R., Braciale V. L., Braciale T. J. Antigen-dependent regulation of interleukin 2 receptor expression on cloned human cytotoxic T lymphocytes. J Immunol. 1984 Oct;133(4):1966–1969. [PubMed] [Google Scholar]
  8. Kaufmann S. H., Hug E., De Libero G. Listeria monocytogenes-reactive T lymphocyte clones with cytolytic activity against infected target cells. J Exp Med. 1986 Jul 1;164(1):363–368. doi: 10.1084/jem.164.1.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kotani H., Mitsuya H., Jarrett R. F., Yenokida G. G., James S. P., Strober W. An autoreactive T cell clone that can be activated to provide both helper and suppressor function. J Immunol. 1986 Mar 15;136(6):1951–1959. [PubMed] [Google Scholar]
  10. Lowenthal J. W., Tougne C., MacDonald H. R., Smith K. A., Nabholz M. Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone. J Immunol. 1985 Feb;134(2):931–939. [PubMed] [Google Scholar]
  11. Mustafa A. S., Godal T. BCG-induced suppressor T cells optimal conditions for in vitro induction and mode of action. Clin Exp Immunol. 1985 Dec;62(3):474–481. [PMC free article] [PubMed] [Google Scholar]
  12. Mustafa A. S., Godal T. In vitro induction of human suppressor T cells by mycobacterial antigens. BCG activated OKT4+ cells mediate suppression of antigen induced T cell proliferation. Clin Exp Immunol. 1983 Apr;52(1):29–37. [PMC free article] [PubMed] [Google Scholar]
  13. Mustafa A. S., Oftung F., Gill H. K., Natvig I. Characteristics of human T-cell clones from BCG and killed M. leprae vaccinated subjects and tuberculosis patients. Recognition of recombinant mycobacterial antigens. Lepr Rev. 1986 Dec;57 (Suppl 2):123–130. [PubMed] [Google Scholar]
  14. Müllbacher A., Parish C. R., Mundy J. P. An improved colorimetric assay for T cell cytotoxicity in vitro. J Immunol Methods. 1984 Mar 30;68(1-2):205–215. doi: 10.1016/0022-1759(84)90151-0. [DOI] [PubMed] [Google Scholar]
  15. Nakamura M., Ross D. T., Briner T. J., Gefter M. L. Cytolytic activity of antigen-specific T cells with helper phenotype. J Immunol. 1986 Jan;136(1):44–47. [PubMed] [Google Scholar]
  16. Parish C. R., Müllbacher A. Automated colorimetric assay for T cell cytotoxicity. J Immunol Methods. 1983 Mar 11;58(1-2):225–237. doi: 10.1016/0022-1759(83)90277-6. [DOI] [PubMed] [Google Scholar]
  17. Pawelec G., Wernet P., Rosenlund R., Blaurock M., Schneider E. M. Strong lymphoproliferative suppressive function of PLT clones specific for SB-like antigens. Hum Immunol. 1984 Mar;9(3):145–157. doi: 10.1016/0198-8859(84)90042-9. [DOI] [PubMed] [Google Scholar]
  18. Unanue E. R., Beller D. I., Lu C. Y., Allen P. M. Antigen presentation: comments on its regulation and mechanism. J Immunol. 1984 Jan;132(1):1–5. [PubMed] [Google Scholar]
  19. Waldmann T. A. The structure, function, and expression of interleukin-2 receptors on normal and malignant lymphocytes. Science. 1986 May 9;232(4751):727–732. doi: 10.1126/science.3008337. [DOI] [PubMed] [Google Scholar]
  20. Wee S. L., Bach F. H. Functionally distinct human T cell clones that produce lymphokines with IL-2-like activity. Hum Immunol. 1984 Mar;9(3):175–188. doi: 10.1016/0198-8859(84)90044-2. [DOI] [PubMed] [Google Scholar]

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