Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1986 Sep;65(3):598–604.

Stimulating and differentiation factors for human B lymphocytes in systemic lupus erythematosus.

E Martinez-Cordero, J Alcocer-Varela, D Alarcon-Segovia
PMCID: PMC1542492  PMID: 3490941

Abstract

T cells from 18 untreated SLE patients produced significantly more B cell growth factor (BCGF) than did those from normal subjects. Those from SLE patients with active disease produced significantly more than did those from patients with inactive disease. The response to BCGF of SAC-stimulated B lymphocytes from SLE patients was higher than that of B lymphocytes from normal individuals. Similarly preactivated B cells from five of seven SLE patients also proliferated upon the addition of interleukin 1 (IL-1) whereas those of normal subjects did not. Simultaneous addition of IL-1 and BCGF had a synergistic proliferative effect on B cells from two of seven SLE patients but not on any of the controls. Interleukin 2 (IL-2) had no proliferative effect in either SLE or normal B cells. Supernatant fractions from T cells of seven of 10 patients with active SLE and three of 10 with inactive SLE induced more IgG production by CESS cells than did those of normal subjects indicating a higher production of B cell differentiation factor by SLE T cells than by those of controls. Our findings may explain the reported preactivation and predifferentiation of peripheral blood B cells from SLE patients and give insight into the mechanisms leading to the production of autoantibodies in this disease.

Full text

PDF
603

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdou N. I., Sagawa A., Pascual E., Hebert J., Sadeghee S. Suppressor T-cell abnormality in idiopathic systemic lupus erythematosus. Clin Immunol Immunopathol. 1976 Sep;6(2):192–199. doi: 10.1016/0090-1229(76)90110-0. [DOI] [PubMed] [Google Scholar]
  2. Alarcon-Segovia D., Alcocer-Varela J., Diaz-Jouanen E. The connective tissue diseases as disorders of immune regulation. Clin Rheum Dis. 1985 Dec;11(3):451–469. [PubMed] [Google Scholar]
  3. Alarcón-Segovia D., Llorente L., Fishbein E., Díaz-Jouanen E. Abnormalities in the content of nucleic acids of peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Arthritis Rheum. 1982 Mar;25(3):304–317. doi: 10.1002/art.1780250308. [DOI] [PubMed] [Google Scholar]
  4. Alcocer-Varela J., Alarcón-Segovia D. Decreased production of and response to interleukin-2 by cultured lymphocytes from patients with systemic lupus erythematosus. J Clin Invest. 1982 Jun;69(6):1388–1392. doi: 10.1172/JCI110579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Alcocer-Varela J., Laffon A., Alarcón-Segovia D. Defective monocyte production of, and T lymphocyte response to, interleukin-1 in the peripheral blood of patients with systemic lupus erythematosus. Clin Exp Immunol. 1984 Jan;55(1):125–132. [PMC free article] [PubMed] [Google Scholar]
  6. Blaese R. M., Grayson J., Steinberg A. D. Increased immunoglobulin-secreting cells in the blood of patients with active systemic lupus erythematosus. Am J Med. 1980 Sep;69(3):345–350. doi: 10.1016/0002-9343(80)90003-0. [DOI] [PubMed] [Google Scholar]
  7. Bresnihan B., Jasin H. E. Suppressor function of peripheral blood mononuclear cells in normal individuals and in patients with systemic lupus erythematosus. J Clin Invest. 1977 Jan;59(1):106–116. doi: 10.1172/JCI108607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cupps T. R., Gerrard T. L., Falkoff R. J., Whalen G., Fauci A. S. Effects of in vitro corticosteroids on B cell activation, proliferation, and differentiation. J Clin Invest. 1985 Feb;75(2):754–761. doi: 10.1172/JCI111757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Delfraissy J. F., Segond P., Galanaud P., Wallon C., Massias P., Dormont J. Depressed primary in vitro antibody response in untreated systemic lupus erythematosus. T helper cell defect and lack of defective suppressor cell function. J Clin Invest. 1980 Jul;66(1):141–148. doi: 10.1172/JCI109827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Falkoff R. J., Zhu L. P., Fauci A. S. Separate signals for human B cell proliferation and differentiation in response to Staphylococcus aureus: evidence for a two-signal model of B cell activation. J Immunol. 1982 Jul;129(1):97–102. [PubMed] [Google Scholar]
  11. Ginsburg W. W., Finkelman F. D., Lipsky P. E. Circulating and pokeweed mitogen-induced immunoglobulin-secreting cells in systemic lupus erythematosus. Clin Exp Immunol. 1979 Jan;35(1):76–88. [PMC free article] [PubMed] [Google Scholar]
  12. Hirose T., Hara M., Kitani A., Hirose W., Norioka K., Kawagoe M., Nakamura H. Abnormal production of and response to B-cell growth factor and B-cell differentiation factor in patients with systemic lupus erythematosus. Scand J Immunol. 1985 Feb;21(2):141–150. doi: 10.1111/j.1365-3083.1985.tb01413.x. [DOI] [PubMed] [Google Scholar]
  13. Howard M., Paul W. E. Regulation of B-cell growth and differentiation by soluble factors. Annu Rev Immunol. 1983;1:307–333. doi: 10.1146/annurev.iy.01.040183.001515. [DOI] [PubMed] [Google Scholar]
  14. Kehrl J. H., Muraguchi A., Butler J. L., Falkoff R. J., Fauci A. S. Human B cell activation, proliferation and differentiation. Immunol Rev. 1984 Apr;78:75–96. doi: 10.1111/j.1600-065x.1984.tb00477.x. [DOI] [PubMed] [Google Scholar]
  15. Kishimoto T. Factors affecting B-cell growth and differentiation. Annu Rev Immunol. 1985;3:133–157. doi: 10.1146/annurev.iy.03.040185.001025. [DOI] [PubMed] [Google Scholar]
  16. Linker-Israeli M., Bakke A. C., Quismorio F. P., Jr, Horwitz D. A. Correction of interleukin-2 production in patients with systemic lupus erythematosus by removal of spontaneously activated suppressor cells. J Clin Invest. 1985 Feb;75(2):762–768. doi: 10.1172/JCI111758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Melendro E. I., Saldate C., Rivero S. J., Alarcon-Segovia D. T-cell subpopulations in the peripheral blood of patients with connective tissue diseases as determined by flow cytometry using monoclonal antibodies. Clin Immunol Immunopathol. 1983 Jun;27(3):340–347. doi: 10.1016/0090-1229(83)90086-7. [DOI] [PubMed] [Google Scholar]
  18. Ruiz-Arguelles A., Alarcón-Segovia D., Llorente L., Del Giudice-Knipping J. A. Heterogeneity of the spontaneously expanded and mitogen-induced generation of suppressor cell function of T cells on B cells in systemic lupus erythematosus. Arthritis Rheum. 1980 Sep;23(9):1004–1009. doi: 10.1002/art.1780230907. [DOI] [PubMed] [Google Scholar]
  19. Sredni B., Sieckmann D. G., Kumagai S., House S., Green I., Paul W. E. Long-term culture and cloning of nontransformed human B lymphocytes. J Exp Med. 1981 Nov 1;154(5):1500–1516. doi: 10.1084/jem.154.5.1500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  21. Tsudo M., Uchiyama T., Uchino H. Expression of Tac antigen on activated normal human B cells. J Exp Med. 1984 Aug 1;160(2):612–617. doi: 10.1084/jem.160.2.612. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES