Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1987 May;68(2):427–436.

Mouse anti-benzylpenicilloyl IgE monoclonal antibody: preparation, characterization and cross-reactivity.

H Fukushima 1, R Misaki 1, M Takeuchi 1, Y Niinomi 1, M Harada 1
PMCID: PMC1542707  PMID: 3652521

Abstract

Anti-benzylpenicilloyl (BPO-) monoclonal antibody of the IgE class was prepared from spleens of immune C57BL/6 mice whose sera reacted with BPO-hapten, penicillin G(PCG) polymer, cephalothin (CET)-hapten and CET polymer. Affinity chromatography experiments showed that the haptenic specificity of the IgE monoclonal antibody (designated BIE-13CE) was directed mainly to phenylacetyl portion of BPO group. BIE-13CE antibody reacted on passive cutaneous anaphylaxis (PCA) assay with BPO-hapten, CET-hapten, cephaloridine-hapten and CET polymer, but did not react with PCG polymer, ampicillin-hapten, or cefazolin-hapten. These results indicated that the sera of the immune C57BL/6 mice contained IgE antibodies capable of cross-reacting at the monoclonal antibody level with various forms of eliciting antigens and that the cross-reactivity of the antibody could be ascribed essentially to the structural similarity of acyl side chains of the antibiotics. The structure of the CET polymer is also discussed in terms of its PCA reactivity with the monoclonal antibody and analytical and spectral data of the polymer.

Full text

PDF
427

Images in this article

430Fig. 1
on p.430

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAHAM E. P., NEWTON G. G. The structure of cephalesporin C. Biochem J. 1961 May;79:377–393. doi: 10.1042/bj0790377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abraham G. N., Petz L. D., Fudenberg H. H. Immunohaematological cross-allergenicity between penicillin and cephalothin in humans. Clin Exp Immunol. 1968 May;3(4):343–357. [PMC free article] [PubMed] [Google Scholar]
  3. Ahlstedt S., Kristofferson A., Pettersson E. Antigens in penicillin allergy. III. Antigen and antibody levels in mice treated with pure and contaminated penicillins. Int Arch Allergy Appl Immunol. 1979;58(1):20–29. [PubMed] [Google Scholar]
  4. Assem E. S., Vickers M. R. Tests for penicillin allergy in man. II. The immunological cross-reaction between penicillins and cephalosporins. Immunology. 1974 Aug;27(2):255–269. [PMC free article] [PubMed] [Google Scholar]
  5. Atsumi T., Adachi M., Kinoshita Y., Kawasaki M., Horiuchi Y. The heterogeneity of combining sites of anti-benzylpenicilloyl antibodies obtained from individual rabbits: changes in combining sites of gammaG and gammaM antibodies during the immune response. J Immunol. 1968 Nov;101(5):1016–1022. [PubMed] [Google Scholar]
  6. Atsumi T., Nishida K., Kinoshita Y., Shibata K., Horiuchi Y. The heterogeneity of combining sites of anti-benzylpenicilloyl antibodies obtained from individual rabbits: fractionation of antibodies with a specific immunoadsorbent. J Immunol. 1967 Dec;99(6):1286–1293. [PubMed] [Google Scholar]
  7. Batchelor F. R., Dewdney J. M., Weston R. D., Wheeler A. W. The immunogenicity of cephalosporin derivatives and their cross-reaction with penicillin. Immunology. 1966 Jan;10(1):21–33. [PMC free article] [PubMed] [Google Scholar]
  8. Bundgaard H., Larsen C. Polymerization of penicillins. IV. Separation, isolation and characterization of ampicillin polymers formed in aqueous solution. J Chromatogr. 1977 Feb 1;132(1):51–59. doi: 10.1016/s0021-9673(00)93770-1. [DOI] [PubMed] [Google Scholar]
  9. EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
  10. Eshhar Z., Ofarim M., Waks T. Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity. J Immunol. 1980 Feb;124(2):775–780. [PubMed] [Google Scholar]
  11. Galfrè G., Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981;73(Pt B):3–46. doi: 10.1016/0076-6879(81)73054-4. [DOI] [PubMed] [Google Scholar]
  12. Girard J. P. Common antigenic determinants of penicillin G, ampicillin and the cephalosporins demonstrated in men. Int Arch Allergy Appl Immunol. 1968;33(5):428–438. doi: 10.1159/000230058. [DOI] [PubMed] [Google Scholar]
  13. Goldstein N. I., McIntosh A. H., Fisher P. B., Maramorosch K., Schaffner C. P. In vitro anti-mycoplasmal activity of amphotericin B methyl ester. J Antibiot (Tokyo) 1976 Jun;29(6):656–661. doi: 10.7164/antibiotics.29.656. [DOI] [PubMed] [Google Scholar]
  14. Hamilton-Miller J. M., Newton G. G., Abraham E. P. Products of aminolysis and enzymic hydrolysis of the cephalosporins. Biochem J. 1970 Feb;116(3):371–384. doi: 10.1042/bj1160371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Harada M., Watanabe A., Matsumoto M., Takeuchi M. Induction of IgG1 and IgE responses to protein-conjugated and unconjugated beta-lactam antibiotics in the mouse--efficacy of Freund's complete adjuvant. Experientia. 1985 Dec 15;41(12):1584–1585. doi: 10.1007/BF01964818. [DOI] [PubMed] [Google Scholar]
  16. Harada M., Watanabe A., Takeuchi M. Heterogeneity of cross-reactivity of mouse anti-benzylpenicilloyl antibodies against cephalothin haptens or polymers. Jpn J Pharmacol. 1986 Jan;40(1):182–184. doi: 10.1254/jjp.40.182. [DOI] [PubMed] [Google Scholar]
  17. Harada M., Watanabe A., Takeuchi M., Niinomi Y. Cephalothin and penicillin G polymers as elicitors of rat PCA mediated by mouse IgE antibodies. Jpn J Pharmacol. 1986 Jan;40(1):178–181. doi: 10.1254/jjp.40.178. [DOI] [PubMed] [Google Scholar]
  18. Ishizaka K., Ishizaka T. Mechanisms of reaginic hypersensitivity and IgE antibody response. Immunol Rev. 1978;41:109–148. doi: 10.1111/j.1600-065x.1978.tb01462.x. [DOI] [PubMed] [Google Scholar]
  19. Kristofferson A., Ahlstedt S., Pettersson E., Svärd P. O. Antigens in penicillin allergy. I. A radioimmunoassay for detection of penicilloylated protein contaminants in penicillin preparations. Int Arch Allergy Appl Immunol. 1977;55(1-6):13–22. [PubMed] [Google Scholar]
  20. LEVINE B. B. N(ALPHA-D-PENICILLOYL) AMINES AS UNIVALENT HAPTEN INHIBITORS OF ANTIBODYDEPENDENT ALLERGIC REACTIONS TO PENICILLIN. J Med Pharm Chem. 1962 Sep;91:1025–1034. doi: 10.1021/jm01240a016. [DOI] [PubMed] [Google Scholar]
  21. LEVINE B. B., OVARY Z. Studies on the mechanism of the formation of the penicillin antigen. III. The N-(D-alpha-benzylpenicilloyl) group as an antigenic determinant responsible for hypersensitivity to penicillin G. J Exp Med. 1961 Dec 1;114:875–904. doi: 10.1084/jem.114.6.875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. LEVINE B. B. THE NATURE OF THE ANTIGEN-ANTIBODY COMPLEXES WHICH INITIATE ANAPHYLACTIC REACTIONS. II. THE EFFECT OF MOLECULAR SIZE ON THE ABILITIES OF HOMOLOGOUS MULTIVALENT BENZYLPENICILLOYL HAPTENS TO EVOKE PCA AND PASSIVE ARTHUS REACTIONS IN THE GUINEA PIG. J Immunol. 1965 Jan;94:121–131. [PubMed] [Google Scholar]
  23. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  24. Levine B. B. Antigenicity and cross-reactivity of penicillins and cephalosporins. J Infect Dis. 1973 Oct;128(Suppl):S364–S366. doi: 10.1093/infdis/128.supplement_2.s364. [DOI] [PubMed] [Google Scholar]
  25. Liu F. T., Bohn J. W., Ferry E. L., Yamamoto H., Molinaro C. A., Sherman L. A., Klinman N. R., Katz D. H. Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization. J Immunol. 1980 Jun;124(6):2728–2737. [PubMed] [Google Scholar]
  26. Mota I., Perini A. Homocytotropic and heterocytotropic activity of mouse IgG1 antibodies. Int Arch Allergy Appl Immunol. 1983;72(3):199–205. doi: 10.1159/000234868. [DOI] [PubMed] [Google Scholar]
  27. Mota I., Wong D. Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization. Life Sci. 1969 Aug 15;8(16):813–820. doi: 10.1016/0024-3205(69)90099-x. [DOI] [PubMed] [Google Scholar]
  28. Munro A. C., Dewdney J. M., Smith H., Wheeler A. W. Antigenic properties of polymers formed by beta-lactam antibiotics. Int Arch Allergy Appl Immunol. 1976;50(2):192–205. doi: 10.1159/000231496. [DOI] [PubMed] [Google Scholar]
  29. Muranaka M., Tadokoro K., Hirai K., Koizumi K., Fukuba S., Suzuki S. IgE antibodies produced in mice instrumental in analyses of antigenicity of cephalothin preparation. Int Arch Allergy Appl Immunol. 1980;63(3):275–283. doi: 10.1159/000232637. [DOI] [PubMed] [Google Scholar]
  30. Nagasawa H. T., Goon D. J., DeMaster E. G. 2,5,5-Trimethylthiazolidine-4-carboxylic acid, a D(-)-penicillamine-directed pseudometabolite of ethanol. Detoxication mechanism for acetaldehyde. J Med Chem. 1978 Dec;21(12):1274–1279. doi: 10.1021/jm00210a019. [DOI] [PubMed] [Google Scholar]
  31. Nishida K., Kinoshita Y., Atsumi T., Shibata K., Horiuchi Y. The analysis of combining sites of rabbit anti-benzylpenicilloyl antibodies. Immunochemistry. 1972 Dec;9(12):1195–1202. doi: 10.1016/0019-2791(72)90293-5. [DOI] [PubMed] [Google Scholar]
  32. Ovary Z., Caiazza S. S., Kojima S. PCA reactions with mouse antibodies in mice and rats. Int Arch Allergy Appl Immunol. 1975;48(1):16–21. doi: 10.1159/000231289. [DOI] [PubMed] [Google Scholar]
  33. Petersen B. H., Graham J. Immunologic cross-reactivity of cephalexin and penicillin. J Lab Clin Med. 1974 Jun;83(6):860–870. [PubMed] [Google Scholar]
  34. Petz L. D. Immunologic cross-reactivity between penicillins and cephalosporins: a review. J Infect Dis. 1978 May;137 (Suppl):S74–S79. doi: 10.1093/infdis/137.supplement.s74. [DOI] [PubMed] [Google Scholar]
  35. Schwartz M. A. Chemical aspects of penicillin allergy. J Pharm Sci. 1969 Jun;58(6):643–661. doi: 10.1002/jps.2600580602. [DOI] [PubMed] [Google Scholar]
  36. Shibata K., Atsumi T., Horiuchi Y., Mashimo K. Immunological cross-reactivities of cephalothin and its related compounds with benzylpenicillin (penicillin G). Nature. 1966 Oct 22;212(5060):419–420. doi: 10.1038/212419a0. [DOI] [PubMed] [Google Scholar]
  37. Siskind G. W., Benacerraf B. Cell selection by antigen in the immune response. Adv Immunol. 1969;10:1–50. doi: 10.1016/s0065-2776(08)60414-9. [DOI] [PubMed] [Google Scholar]
  38. Smith H., Marshall A. C. Polymers formed by some beta-lactam antibiotics. Nature. 1971 Jul 2;232(5305):45–46. doi: 10.1038/232045a0. [DOI] [PubMed] [Google Scholar]
  39. Sogn D. D. Penicillin allergy. J Allergy Clin Immunol. 1984 Oct;74(4 Pt 2):589–593. doi: 10.1016/0091-6749(84)90111-8. [DOI] [PubMed] [Google Scholar]
  40. Solley G. O., Gleich G. J., Van Dellen R. G. Penicillin allergy: clinical experience with a battery of skin-test reagents. J Allergy Clin Immunol. 1982 Feb;69(2):238–244. doi: 10.1016/0091-6749(82)90105-1. [DOI] [PubMed] [Google Scholar]
  41. Tung A. S. Production, purification, and characterization of antigen-specific murine monoclonal antibodies of IgE class. Methods Enzymol. 1983;92:47–66. doi: 10.1016/0076-6879(83)92009-8. [DOI] [PubMed] [Google Scholar]
  42. Warrington R. J., Simons F. E., Ho H. W., Gorski B. A. Diagnosis of penicillin allergy by skin testing: the Manitoba experience. Can Med Assoc J. 1978 Apr 8;118(7):787–791. [PMC free article] [PubMed] [Google Scholar]
  43. de Haan P., de Jonge A. J., Verbrugge T., Boorsma D. M. Three epitope-specific monoclonal antibodies against the hapten penicillin. Int Arch Allergy Appl Immunol. 1985;76(1):42–46. doi: 10.1159/000233659. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES