Skip to main content
PMC home page
Gut logoLink to Gut
. 1970 Oct;11(10):859–866. doi: 10.1136/gut.11.10.859

The interrelationships of pancreatic enzymes in human duodenal aspirate 1

D M Goldberg, K G Wormsley
PMCID: PMC1553158  PMID: 5485836

Abstract

The interrelationships of proteolytic enzymes and amylase have been studied in the duodenal aspirate obtained from subjects with normal and abnormal pancreatic function during stimulation with secretin and pancreozymin. While the relationship of trypsin to chymotrypsin was independent of stimulus and presence of pancreatic disease the ratio of proteolytic enzymes to amylase rose when the degree of stimulation of the pancreas was increased. Patients with recent acute pancreatitis and with chronic pancreatitis tended to have more severe impairment of secretion of proteolytic enzymes than of amylase. In routine tests of pancreatic function both proteolytic and non-proteolytic enzymes should be measured, both because an abnormal ratio may be of diagnostic significance and because the two different groups of enzymes provide mutual checks of the secretory capacity of pancreatic enzymes.

Full text

PDF
861

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ammann R. W., Tagwecher E., Kashiwagi H., Rosenmund H. Diagnostic value of fecal chymotrypsin and trypsin assessment for detection of pancreatic disease. A comparative study. Am J Dig Dis. 1968 Feb;13(2):123–146. doi: 10.1007/BF02232955. [DOI] [PubMed] [Google Scholar]
  2. BURTON P., EVANS D. G., HARPER A. A., HOWATH T., OLEESKY S., SCOTT J. E., VARLEY H. A test of pancreatic function in man based on the analysis of duodenal contents after administration of secretin and pancreozymin. Gut. 1960 Jun;1:111–124. doi: 10.1136/gut.1.2.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CREUTZFELDT W. FUNKTIONSDIAGNOSTIK BEI ERKRANKUNGEN DES EXOKRINEN PANKREAS. Verh Dtsch Ges Inn Med. 1964;70:781–801. [PubMed] [Google Scholar]
  4. Choi H. J., Goldstein F., Wirts C. W., Menduke H. Normal duodenal trypsin values in response to secretin-pancreozymin stimulation with preliminary data in patients with pancreatic disease. Gastroenterology. 1967 Sep;53(3):397–402. [PubMed] [Google Scholar]
  5. GIBBS G. E. Secretin tests with bilumen gastroduodenal drainage in infants and children. Pediatrics. 1950 Jun;5(6):941–946. [PubMed] [Google Scholar]
  6. GOWENLOCK A. H. The estimation of tryptic activity in duodenal contents. Biochem J. 1953 Jan;53(2):274–277. doi: 10.1042/bj0530274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GUTH P. H., KOMAROV S. A., SHAY H., STYLE C. Z. Relationship between protein nitrogen, proteolytic, amylolytic and lipolytic enzymes in canine pancreatic juice obtained under various conditions of stimulation. Am J Physiol. 1956 Nov;187(2):207–223. doi: 10.1152/ajplegacy.1956.187.2.207. [DOI] [PubMed] [Google Scholar]
  8. Goldberg D. M., Campbell R., Roy A. D. Studies on the binding of trypsin and chymotrypsin by human intestinal mucosa. Scand J Gastroenterol. 1969;4(3):217–226. [PubMed] [Google Scholar]
  9. Hershfield N. B., Lind J. F., Hildes J. A. Clinical correlations with pancreatic function tests. Can Med Assoc J. 1968 Jan 27;98(4):185–188. [PMC free article] [PubMed] [Google Scholar]
  10. Ribet A., Pascal J. P., Vaysse N. Recherche de la capacité enzymatique maxima du pancr-eas exocrine humain normal sous perfusion continue de sécrétine et de pancréozymine. Biol Gastroenterol (Paris) 1968;2:163–170. [PubMed] [Google Scholar]
  11. Rick W. Zur Pathologie der Enzymsekretion des Pankreas. Acta Gastroenterol Belg. 1965 May-Jun;28(5):389–400. [PubMed] [Google Scholar]
  12. Rothman S. S. "Non-parallel transport" of enzyme protein by the pancreas. Nature. 1967 Feb 4;213(5075):460–462. doi: 10.1038/213460a0. [DOI] [PubMed] [Google Scholar]
  13. Sarles H., Figarella C., Prezelin G., Souville C. Comportement différent de la lipase, de l'amylase et des enzymes protéolytiques pancréatiques après différents modes d'excitation du pancréas humain. Bull Soc Chim Biol (Paris) 1966;48(8):951–957. [PubMed] [Google Scholar]
  14. Wormsley K. G. Response to secretin in man. Gastroenterology. 1968 Feb;54(2):197–209. [PubMed] [Google Scholar]
  15. Worning H., Müllertz S. pH and pancreatic enzymes in the human duodenum during digestion of a standard meal. Scand J Gastroenterol. 1966;1(4):268–283. doi: 10.1080/00365521.1966.11800642. [DOI] [PubMed] [Google Scholar]
  16. Zieve L., Silvis S. E., Mulford B., Blackwood W. D. Secretion of pancreatic enzymes. I. Response to secretin and pancreozymin. Am J Dig Dis. 1966 Sep;11(9):671–684. doi: 10.1007/BF02239420. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES