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. 1972 Nov;12(3):351–356.

Variables in the quantitation of the mixed leucocyte response by tritiated thymidine uptake

A R Shons, E E Etheredge, J S Najarian
PMCID: PMC1553588  PMID: 4264647

Abstract

We have used 3H-thymidine uptake to quantitate the mixed leucocyte response. The technique of 3H-thymidine labelling is an important variable in the total mixed leucocyte culture technique. The effect of variations in the specific activity of 3H-thymidine used, the amount of radioactivity added per culture and the time of exposure to 3H-thymidine was studied using a micro-MLC method. Optimum quantitative and qualitative discrimination was obtained by the addition of high specific activity thymidine for an exposure period of 18–24 hr.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bain B. Tritiated-thymidine uptake in mixed leucocyte cultures: effect of specific activity and exposure time. Clin Exp Immunol. 1970 Feb;6(2):255–262. [PMC free article] [PubMed] [Google Scholar]
  2. Hartzman R. J., Segall M., Bach M. L., Bach F. H. Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report. Transplantation. 1971 Mar;11(3):268–273. doi: 10.1097/00007890-197103000-00005. [DOI] [PubMed] [Google Scholar]

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