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. 1972 Aug;11(4):507–522.

A defect of B-lymphocyte transport of aggregated HGG into germinal centres in NZB and NZB×NZW F1 hybrid mice

D G de Jesus, E J Holborow, J C Brown
PMCID: PMC1553699  PMID: 4117458

Abstract

Previous experiments have shown (i) that the localization of intravenously injected heat aggregated human γ-globulin (HGG) in the germinal centres of normal mice provides a model for studying the natural uptake of circulating immune complexes in these areas, and (ii) that the aggregated HGG is carried into germinal centres by lymphocytes which have receptors for altered γ-globlin.

Evidence from thymus-cell depletion experiments is now presented which suggests that the lymphocytes concerned are bone-marrow-derived B cells. Defective localization was found in NZB and NZB × NZW F1 hybrids at different ages and the onset of the autoimmunity and the appearance of histological abnormalities in the spleen. As disease develops it progresses to a complete inability to localize complexes in germinal centres. It is concluded that a functional defect of the bone marrow-derived lymphocyte population exists in these mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown J. C., De Jesus D. G., Holborow E. J., Harris G. Lymphocyte-mediated transport of aggregated human gamma-globulin into germinal centre areas of normal mouse spleen. Nature. 1970 Oct 24;228(5269):367–369. doi: 10.1038/228367a0. [DOI] [PubMed] [Google Scholar]
  2. Brown J. C., Schwab J. H., Holborow E. J. The localization of immunoglobulin and immune complexes in lymphoid tissue. Immunology. 1970 Sep;19(3):401–415. [PMC free article] [PubMed] [Google Scholar]
  3. Cantor H., Asofsky R., Talal N. Synergy among lymphoid cells mediating the graft-versus-host response. I. Synergy in graft-versus-host reactions produced by cells from NZB-Bl mice. J Exp Med. 1970 Feb;131(2):223–234. doi: 10.1084/jem.131.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carter M. J., Parsons D. S. Carbonic anhydrase activity of mucosa of small intestine and colon. Nature. 1968 Jul 13;219(5150):176–177. doi: 10.1038/219176a0. [DOI] [PubMed] [Google Scholar]
  5. Cerottini J. C., Lambert P. H., Dixon F. J. Comparison of the immune responsiveness of NZB and NZB X NZW F1 hybrid mice with that of other strains of mice. J Exp Med. 1969 Nov 1;130(5):1093–1105. doi: 10.1084/jem.130.5.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies A. J. The thymus and the cellular basis of immunity. Transplant Rev. 1969;1:43–91. doi: 10.1111/j.1600-065x.1969.tb00136.x. [DOI] [PubMed] [Google Scholar]
  7. Denman A. M., Denman E. J. Depletion of long-lived lymphocytes in old New Zealand black mice. Clin Exp Immunol. 1970 Apr;6(4):457–472. [PMC free article] [PubMed] [Google Scholar]
  8. Denman A. M., Denman E. J., Holborow E. J. Effect of anti-lymphocyte globulin on kidney disease in (N.Z.B. x N.Z.W.) F 1 mice. Lancet. 1966 Oct 15;2(7468):841–843. doi: 10.1016/s0140-6736(66)92260-4. [DOI] [PubMed] [Google Scholar]
  9. East J. Immunopathology and neoplasms in New Zealand black (NZB) and SJL-J mice. Prog Exp Tumor Res. 1970;13:84–134. doi: 10.1159/000386038. [DOI] [PubMed] [Google Scholar]
  10. East J., de Sousa M. A., Parrott D. M. Immunopathology of New Zealand black (NZB) mice. Transplantation. 1965 Nov;3(6):711–729. doi: 10.1097/00007890-196511000-00003. [DOI] [PubMed] [Google Scholar]
  11. Evans M. M., Williamson W. G., Irvine W. J. The appearance of immunological competence at an early age in New Zealand black mice. Clin Exp Immunol. 1968 Jun;3(5):375–383. [PMC free article] [PubMed] [Google Scholar]
  12. Gazdar A. F., Beitzel W., Talal N. The age related responses of New Zealand mice to a murine sarcoma virus. Clin Exp Immunol. 1971 Mar;8(3):501–509. [PMC free article] [PubMed] [Google Scholar]
  13. Greenwood B. M., Brown J. C., De Jesus D. G., Holborow E. J. Immunosuppression in murine malaria. II. The effect on reticulo-endothelial and germinal centre function. Clin Exp Immunol. 1971 Sep;9(3):345–354. [PMC free article] [PubMed] [Google Scholar]
  14. Greenwood B. M., Playfair J. H., Torrigiani G. Immunosuppression in murine malaria. I. General characteristics. Clin Exp Immunol. 1971 Mar;8(3):467–478. [PMC free article] [PubMed] [Google Scholar]
  15. Howie J. B., Helyer B. J. The immunology and pathology of NZB mice. Adv Immunol. 1968;9:215–266. doi: 10.1016/s0065-2776(08)60444-7. [DOI] [PubMed] [Google Scholar]
  16. Lambert P. H., Dixon F. J. Pathogenesis of the glomerulonephritis of NZB/W mice. J Exp Med. 1968 Mar 1;127(3):507–522. doi: 10.1084/jem.127.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Leventhal B. G., Talal N. Response of NZB and NZB-NZW spleen cells to mitogenic agents. J Immunol. 1970 Apr;104(4):918–923. [PubMed] [Google Scholar]
  18. Morton J. I., Siegel B. V. Response of NZB mice to foreign antigen and development of autoimmune disease. J Reticuloendothel Soc. 1969 Feb;6(1):78–93. [PubMed] [Google Scholar]
  19. Playfair J. H. Strain differences in the immune response of mice. I. The neonatal response to sheep red cells. Immunology. 1968 Jul;15(1):35–50. [PMC free article] [PubMed] [Google Scholar]
  20. Rodey G. E., Good R. A., Yunis E. J. Progressive loss in vitro of cellular immunity with ageing in strains of mice susceptible to autoimmune disease. Clin Exp Immunol. 1971 Sep;9(3):305–311. [PMC free article] [PubMed] [Google Scholar]
  21. Salomon J. C., Benveniste J. The immune response in NZBxNZW F1 hybrid mice. Clin Exp Immunol. 1969 Feb;4(2):213–225. [PMC free article] [PubMed] [Google Scholar]
  22. Staples P. J., Steinberg A. D., Talal N. Induction of immunologic tolerance in older New Zealand mice repopulated with young spleen, bone marrow, or thymus. J Exp Med. 1970 Jun 1;131(6):1223–1238. doi: 10.1084/jem.131.6.1223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Staples P. J., Talal N. Relative inability to induce tolerance in adult NZB and NZB-NZW F1 mice. J Exp Med. 1969 Jan 1;129(1):123–139. doi: 10.1084/jem.129.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Steinberg A. D., Law L. D., Talal N. The role of NZB-NZW F1 thymus in experimental tolerance and auto-immunity. Arthritis Rheum. 1970 Jul-Aug;13(4):369–377. doi: 10.1002/art.1780130402. [DOI] [PubMed] [Google Scholar]
  25. Stutman O., Yunis E. J., Good R. A. Deficient immunologic functions of NZB mice. Proc Soc Exp Biol Med. 1968 Apr;127(4):1204–1207. doi: 10.3181/00379727-127-32910. [DOI] [PubMed] [Google Scholar]
  26. Sutherland D. E., Archer O. K., Peterson R. D., Eckert E., Good R. A. Development of "autoimmune processes" in rabbits after neonatal removal of central lymphoid tissue. Lancet. 1965 Jan 16;1(7377):130–133. doi: 10.1016/s0140-6736(65)91091-3. [DOI] [PubMed] [Google Scholar]
  27. Taylor R. B. Cellular cooperation in the antibody response of mice to two serum albumins: specific function of thymus cells. Transplant Rev. 1969;1:114–149. doi: 10.1111/j.1600-065x.1969.tb00138.x. [DOI] [PubMed] [Google Scholar]
  28. Teague P. O., Friou G. J. Antinuclear antibodies in mice. II. Transmission with spleen cells; inhibition or prevention with thymus or spleen cells. Immunology. 1969 Nov;17(5):665–675. [PMC free article] [PubMed] [Google Scholar]
  29. Teague P. O., Yunis E. J., Rodey G., Fish A. J., Stutman O., Good R. A. Autoimmune phenomena and renal disease in mice. Role of thymectomy, aging, and involution of immunologic capacity. Lab Invest. 1970 Feb;22(2):121–130. [PubMed] [Google Scholar]
  30. Thivolet J., Monier J. C., Ruel J. P., Richard M. H. Antinuclear autoantibodies in Swiss mice thymectomized at birth. Nature. 1967 Jun 10;214(5093):1134–1136. doi: 10.1038/2141134a0. [DOI] [PubMed] [Google Scholar]
  31. Zatz M. M., Mellors R. C., Lance E. M. Changes in lymphoid populations of ageing CBA and NZB mice. Clin Exp Immunol. 1971 Mar;8(3):491–500. [PMC free article] [PubMed] [Google Scholar]
  32. de Vries M. J., Hijmans W. A deficient development of the thymic epithelium and auto-immune disease in NZB mice. J Pathol Bacteriol. 1966 Apr;91(2):487–494. doi: 10.1002/path.1700910223. [DOI] [PubMed] [Google Scholar]

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