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. 1973 Feb;13(2):279–292.

Immunobiology of the autoantibody response. I. Circulating analogues of erythrocyte autoantigens and heterogeneity of the autoimmune response of NZB mice

E Linder, T S Edgington
PMCID: PMC1553717  PMID: 4120854

Abstract

Anti-erythrocyte autoantibodies of two distinct antigenic specificities can be recovered from immunoglobulin-coated NZB erythrocytes. These autoantibodies react with either exposed (X) or hidden (HB) antigenic determinants on murine red cells and both types can be neutralized by mouse plasma. Antibody neutralization by plasma is dependent on two distinct plasma activities which appear to be soluble analogues of the erythrocyte surface autoantigens X and HB. The soluble erythrocyte antigens SEA-X and SEA-HB can be separated by molecular exclusion chromatography, with approximate sizes of 2 × 105 for SEA–X and ≥ 5 × 106 for SEA-HB. The presence of these soluble erythrocyte autoantigens in the plasmas of `autoimmune' NZB mice has to be considered in relation to the immunologic homeostasis of these autoantibody responses and pathogenesis of both the autoimmune haemolytic anaemia and the autologous immune complex disease of these mice.

Individual anti-erythrocyte autoantibodies of both anti-X and anti-HB types exhibit discrete and individually distinctive differences in affinity for autoantigen and also in respect to antigenic determinant specificity. These results suggest that factors which control the genetic selection of these responses in an inbred strain do not prescribe a single specific B lymphoid cell clone; but rather it appears that a number of different autoimmunocompetent B lymphocyte clones with differing affinity and determinant specificity for given autoantigen molecules may be dere-pressed by other events in the recruitment of the autoantibody responses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDRESEN E. Blood groups in pigs. Ann N Y Acad Sci. 1962 May 3;97:205–225. [PubMed] [Google Scholar]
  2. ANDRESEN E. Blood groups in pigs. Ann N Y Acad Sci. 1962 May 3;97:205–225. [PubMed] [Google Scholar]
  3. Allison A. C., Denman A. M., Barnes R. D. Cooperating and controlling functions of thymus-derived lymphocytes in relation to autoimmunity. Lancet. 1971 Jul 17;2(7716):135–140. doi: 10.1016/s0140-6736(71)92306-3. [DOI] [PubMed] [Google Scholar]
  4. Barnes R. D., Tuffrey M., Kingman J., Risdon R. A. The disease of the NZB mouse. Examination of exchange ovum transplantation derived NZB and CFW mice. Clin Exp Immunol. 1972 Mar;10(3):493–514. [PMC free article] [PubMed] [Google Scholar]
  5. Benjamin D. C., Weigle W. O. The termination of immunological unresponsiveness to bovine serum albumin in rabbits. I. Quantitative and qualitative response to cross-reacting albumins. J Exp Med. 1970 Jul 1;132(1):66–76. doi: 10.1084/jem.132.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brawn R. J. In vitro desensitization of sensitized murine lymphocytes by a serum factor (soluble antigen?). Proc Natl Acad Sci U S A. 1971 Jul;68(7):1634–1638. doi: 10.1073/pnas.68.7.1634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Costea N., Yakulis V., Heller P. Cold reacting antibody in NZB-B1 mice. Blood. 1970 May;35(5):583–590. [PubMed] [Google Scholar]
  8. EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
  9. Edgington T. S., Glassock R. J., Dixon F. J. Autologous immune-complex pathogenesis of experimental allergic glomerulonephritis. Science. 1967 Mar 17;155(3768):1432–1434. doi: 10.1126/science.155.3768.1432. [DOI] [PubMed] [Google Scholar]
  10. Feldmann M., Easten A. The relationship between antigenic structure and the requirement for thymus-derived cells in the immune response. J Exp Med. 1971 Jul 1;134(1):103–119. doi: 10.1084/jem.134.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gershon R. K., Cohen P., Hencin R., Liebhaber S. A. Suppressor T cells. J Immunol. 1972 Mar;108(3):586–590. [PubMed] [Google Scholar]
  12. Holborow E. J., Barnes R. D., Tuffrey M. A new red-cell autoantibody in NZB mice. Nature. 1965 Aug 7;207(997):601–604. doi: 10.1038/207601a0. [DOI] [PubMed] [Google Scholar]
  13. Howie J. B., Helyer B. J. The immunology and pathology of NZB mice. Adv Immunol. 1968;9:215–266. doi: 10.1016/s0065-2776(08)60444-7. [DOI] [PubMed] [Google Scholar]
  14. LONG G., HOLMES M. C., BURNET F. M. AUTOANTIBODIES PRODUCED AGAINST MOUSE ERYTHROCYTES IN NZB MICE. Aust J Exp Biol Med Sci. 1963 Aug;41:315–322. doi: 10.1038/icb.1963.31. [DOI] [PubMed] [Google Scholar]
  15. Linder E. J., Edgington T. S. New plasma autoantigen and its role in autoimmune disease of New Zealand Black mice. Nat New Biol. 1971 Dec 29;234(52):279–281. doi: 10.1038/newbio234279a0. [DOI] [PubMed] [Google Scholar]
  16. Linder E. J., Edgington T. S. Ultramicro assay of anti-erythrocyte antibodies and erythrocyte antigens. Vox Sang. 1971 Sep;21(3):222–232. doi: 10.1111/j.1423-0410.1971.tb00580.x. [DOI] [PubMed] [Google Scholar]
  17. Mellors R. C., Aoki T., Huebner R. J. Further implication of murine leukemia-like virs in the disorders of NZB mice. J Exp Med. 1969 May 1;129(5):1045–1062. doi: 10.1084/jem.129.5.1045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NORINS L. C., HOLMES M. C. ANTINUCLEAR FACTOR IN MICE. J Immunol. 1964 Jul;93:148–154. [PubMed] [Google Scholar]
  19. Plow E., Edgington T. S. Molecular events responsible for modulation of neoantigenic expression: the cleavage-associated neoantigen of fibrinogen (blood coagulation-fibrinogen cleavage products-fibrinolysis-molecular conformation). Proc Natl Acad Sci U S A. 1972 Jan;69(1):208–212. doi: 10.1073/pnas.69.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. RASMUSEN B. A. Blood groups in sheep. Ann N Y Acad Sci. 1962 May 3;97:306–319. doi: 10.1111/j.1749-6632.1962.tb34645.x. [DOI] [PubMed] [Google Scholar]
  21. Rodey G. E., Good R. A., Yunis E. J. Progressive loss in vitro of cellular immunity with ageing in strains of mice susceptible to autoimmune disease. Clin Exp Immunol. 1971 Sep;9(3):305–311. [PMC free article] [PubMed] [Google Scholar]
  22. Russell P. J., Hicks J. D., Boston L. E., Abbott A. Failure to transfer haemolytic anaemia or glomerulonephritis with cell-free material from NZB mice. Clin Exp Immunol. 1970 Feb;6(2):227–239. [PMC free article] [PubMed] [Google Scholar]
  23. Staples P. J., Talal N. Relative inability to induce tolerance in adult NZB and NZB-NZW F1 mice. J Exp Med. 1969 Jan 1;129(1):123–139. doi: 10.1084/jem.129.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WEINER W. Eluting red-cell antibodies: a method and its application. Br J Haematol. 1957 Jul;3(3):276–283. doi: 10.1111/j.1365-2141.1957.tb05797.x. [DOI] [PubMed] [Google Scholar]
  25. Warner N. L., Wistar R., Jr Immunoglobulins in NZB-BL mice. I. Serum immunoglobulin levels and immunoglobulin class of erythrocyte autoantibody. J Exp Med. 1968 Jan 1;127(1):169–183. doi: 10.1084/jem.127.1.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weigle W. O. Recent observations and concepts in immunological unresponsiveness and autoimmunity. Clin Exp Immunol. 1971 Oct;9(4):437–447. [PMC free article] [PubMed] [Google Scholar]
  27. Weigle W. O. The production of thyroiditis and antibody following injection of unaltered thyroglobulin without adjuvant into rabbits previously stimulated with altered thyroglobulin. J Exp Med. 1965 Dec 1;122(6):1049–1062. doi: 10.1084/jem.122.6.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]

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