Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1973 Mar;13(3):427–437.

Characterization of a serum inhibitor of MLC reactions

R A Gatti, E J Yunis, R A Good
PMCID: PMC1553779  PMID: 4144968

Abstract

Serum from a healthy multigravida female was found to inhibit most MLC combinations tested. Attempts to characterize the inhibitory factor have revealed that it is not lymphocytotoxic, destroyed by heat or directed against HL-A specificities. The inhibitory activity segregates with the 7S IgG fraction, is effective in very small concentrations and consistently fails to inhibit the MLC combinations between cells from the serum donor and those from one other unrelated individual. The blocking effect on MLC occurs during the first 6–9 hr of incubation. Inhibitory activity persists in the donor's serum 16 months after delivery of her last child. An attempt to utilize the inhibitory serum to minimize an expected graft-versus-host (GVH) reaction following bone marrow transplantation to the serum donor's daughter with marrow from the child's father resulted in neither GVH nor a `take' of the graft. The possible effects of the serum on the grafted cells are discussed. While a recognition site for the inhibitory effect remains unknown, it appears to involve a T-cell receptor.

Full text

PDF
428

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bach F. H. Phenotypic expressions of the major histocompatibility locus in man (HL-A): leukocyte antigens and mixed leukocyte culture reactivity. J Exp Med. 1968 Oct 1;128(4):623–637. doi: 10.1084/jem.128.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buckley R. H., Amos D. B., Kremer W. B., Stickel D. L. Incompatible bone-marrow transplantation in lymphopenic immunologic deficiency. Circumvention of fatal graft-versus-host disease by immunologic enhancement. N Engl J Med. 1971 Nov 4;285(19):1035–1042. doi: 10.1056/NEJM197111042851901. [DOI] [PubMed] [Google Scholar]
  3. Buckley R. H., Schiff R. I., Amos D. B. Blocking of autologous and homologous leukocyte responses by human alloimmune plasmas: a possible in vitro correlate of enhancement. J Immunol. 1972 Jan;108(1):34–44. [PubMed] [Google Scholar]
  4. Gatti R. A. Serum inhibitors of lymphocyte responses. Lancet. 1971 Jun 26;1(7713):1351–1352. doi: 10.1016/s0140-6736(71)91906-4. [DOI] [PubMed] [Google Scholar]
  5. Greaves M. F., Torrigiani G., Roitt I. M. Inhibition of human mixed lymphocyte reaction by antibodies to immunoglobulin light chain determinants. Clin Exp Immunol. 1971 Sep;9(3):313–328. [PMC free article] [PubMed] [Google Scholar]
  6. Hattler B. G., Jr, Karesh C., Miller J. Inhibition of the mixed lymphocyte culture response by antibody following successful human renal transplantation. Tissue Antigens. 1971;1(6):270–275. doi: 10.1111/j.1399-0039.1971.tb00105.x. [DOI] [PubMed] [Google Scholar]
  7. Mason S., Warner N. L. The immunoglobulin nature of the antigen recognition site on cells mediating transplantation immunity and delayed hypersentivity. J Immunol. 1970 Mar;104(3):762–765. [PubMed] [Google Scholar]
  8. Oppenheim J. J. Modulation of in vitro lymphocyte transformation by antibodies: enhancement by antigen-antibody complexes and inhibition by antibody excess. Cell Immunol. 1972 Mar;3(3):341–360. doi: 10.1016/0008-8749(72)90243-2. [DOI] [PubMed] [Google Scholar]
  9. Riethmüller G., Rieber E. P., Seeger I. Suppression of graft versus host reaction by univalent anti-immunoglobulin antibody. Nat New Biol. 1971 Apr 21;230(16):248–250. doi: 10.1038/newbio230248a0. [DOI] [PubMed] [Google Scholar]
  10. Sjögren H. O., Hellström I., Bansal S. C., Hellström K. E. Suggestive evidence that the "blocking antibodies" of tumor-bearing individuals may be antigen--antibody complexes. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1372–1375. doi: 10.1073/pnas.68.6.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Storb R., Epstein R. B., Graham T. C., Thomas E. D. Methotrexate regimens for control of graft-versus-host disease in dogs with allogeneic marrow grafts. Transplantation. 1970 Mar;9(3):240–246. doi: 10.1097/00007890-197003000-00007. [DOI] [PubMed] [Google Scholar]
  12. Van der Werf A. J. Are lymphocytotoxic iso-antibodies induced by the early human trophoblast? Lancet. 1971 Mar 20;1(7699):595–595. doi: 10.1016/s0140-6736(71)91191-3. [DOI] [PubMed] [Google Scholar]
  13. Yunis E. J., Amos D. B. Three closely linked genetic systems relevant to transplantation. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3031–3035. doi: 10.1073/pnas.68.12.3031. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES