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. 1973 Oct;15(2):289–302.

Human mixed lymphocyte cultures. Evaluation of microculture technique utilizing the multiple automated sample harvester (MASH)

G B Thurman, D M Strong, A Ahmed, S S Green, K W Sell, R J Hartzman, F H Bach
PMCID: PMC1553877  PMID: 4271568

Abstract

Use of lymphocyte cultures for in vitro studies such as pretransplant histocompatibility testing has established the need for standardization of this technique. A microculture technique has been developed that has facilitated the culturing of lymphocytes and increased the quantity of cultures feasible, while lowering the variation between replicate samples. Cultures were prepared for determination of tritiated thymidine incorporation using a Multiple Automated Sample Harvester (MASH). Using this system, the parameters that influence the in vitro responsiveness of human lymphocytes to allogeneic lymphocytes have been investigated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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