Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1974 Mar;16(3):383–392.

The mitogenic response of human B lymphocytes to phytohaemagglutinin

B Phillips, E Weisrose
PMCID: PMC1553944  PMID: 4619782

Abstract

Further evidence is given here of a mitogenic response to phytohaemagglutinin by human B lymphocytes. Immunofluorescent staining of unfractionated lymphocytes, and thymidine uptake by column purified populations of B and T cells, indicates that the dose–time response of these two populations is comparable. PHA transformation of B lymphocytes appears to be reduced or absent in chronic lymphocytic leukaemia.

Full text

PDF
385

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biberfeld P., Biberfeld G., Perlmann P. Surface immunoglobulin light chain determinants on normal and PHA-stimulated human blood lymphocytes studied by immunofluorescence and electronmicroscopy. Exp Cell Res. 1971 May;66(1):177–189. doi: 10.1016/s0014-4827(71)80027-7. [DOI] [PubMed] [Google Scholar]
  2. Cooper M. D., Lawton A. R., Bockman D. E. Agammaglobulinaemia with B lymphocytes. Specific defect of plasma-cell differentiation. Lancet. 1971 Oct 9;2(7728):791–794. doi: 10.1016/s0140-6736(71)92742-5. [DOI] [PubMed] [Google Scholar]
  3. Doenhoff M. J., Davies A. J., Leuchars E., Wallis V. The thymus and circulating lymphocytes of mice. Proc R Soc Lond B Biol Sci. 1970 Oct 13;176(1042):69–85. doi: 10.1098/rspb.1970.0035. [DOI] [PubMed] [Google Scholar]
  4. Johnston J. M., Wilson D. B. Origin of immunoreactive lymphocytes in rats. Cell Immunol. 1970 Oct;1(4):430–444. doi: 10.1016/0008-8749(70)90019-5. [DOI] [PubMed] [Google Scholar]
  5. Jones G. Lymphocyte activation. I. Expression of theta, H-2 and immunoglobulin determinants on lymphocytes stimulated by phytohaemagglutinin, pokeweed mitogen, concanavalin A or histocompatibility antigen. Clin Exp Immunol. 1972 Nov;12(3):391–402. [PMC free article] [PubMed] [Google Scholar]
  6. Phillips B., Roitt I. M. Evidence for transformation of human B lymphocytes by PHA. Nat New Biol. 1973 Feb 21;241(112):254–256. doi: 10.1038/newbio241254a0. [DOI] [PubMed] [Google Scholar]
  7. Piguet P. F., Vassalli P. Thymus-independent (B) cell proliferation in spleen cell cultures of mouse radiation chimeras stimulated by phytohemagglutinin or allogeneic cells. J Exp Med. 1972 Oct 1;136(4):962–967. doi: 10.1084/jem.136.4.962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ripps C. S., Hirschhorn K. The production of immunoglobulins by human peripheral blood lymphocytes in vitro. Clin Exp Immunol. 1967 Jul;2(4):377–398. [PMC free article] [PubMed] [Google Scholar]
  9. Schlossman S. F., Hudson L. Specific purification of lymphocyte populations on a digestible immunoabsorbent. J Immunol. 1973 Jan;110(1):313–319. [PubMed] [Google Scholar]
  10. Thomas D. B., Phillips B. Membrane antigens specific for human lymphoid cells in the dividing phase. J Exp Med. 1973 Jul 1;138(1):64–70. doi: 10.1084/jem.138.1.64. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vischer T. L. Immunoglobulin-like surface molecules and theta antigen during the specific and non-specific stimulation of mouse spleen cells in vitro. Clin Exp Immunol. 1972 Aug;11(4):523–534. [PMC free article] [PubMed] [Google Scholar]
  12. Wilson J. D., Nossal G. J. Identification of human T and B lymphocytes in normal peripheral blood and in chronic lymphocytic leukaemia. Lancet. 1971 Oct 9;2(7728):788–791. doi: 10.1016/s0140-6736(71)92741-3. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES