Abstract
IgG and IgA, albumin and lactoferrin as well as the semen compartment parameters acid phosphatase, fructose and spermatozoa were determined in separately collected fractions of the same ejaculate of some normal donors. The distribution over the fractions per ejaculate of IgG, IgA and albumin was generally more or less similar to the distribution of acid phosphatase indicating that the bulk of these proteins enters the semen via the prostate and not via the vesicles or testis and epididymis. The distribution of lactoferrin unexpectedly was not clearly related to fructose. IgM could not be detected.
The concentrations in the (eight) total ejaculates expressed as percentages of the serum concentrations were for albumin slightly higher than for IgG, both in the order of 1% and moreover correlated with each other, indicating that IgG reached the seminal fluid in general by transudation from the circulation. The relative IgA concentrations could not be measured exactly but seemed to be slightly higher than of albumin, and not correlated to albumin concentrations, suggesting that local production of IgA may occur also.
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Selected References
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- Ablin R. J., Gonder M. J., Soanes W. A. Localization of immunoglobulins in human prostatic tissue. J Immunol. 1971 Aug;107(2):603–604. [PubMed] [Google Scholar]
- Brandtzaeg P. Unfolding of human secretory immunoglobulin A. Immunochemistry. 1970 Jan;7(1):127–130. doi: 10.1016/0019-2791(70)90037-6. [DOI] [PubMed] [Google Scholar]
- ESTBORN B., SWEDIN B. Localisation of acid phosphatase by starch-gel electrophoresis of fractionally collected human semen. Scand J Clin Lab Invest. 1959;11:235–237. doi: 10.3109/00365515909060442. [DOI] [PubMed] [Google Scholar]
- Fjällbrant B. Interrelation between high levels of sperm antibodies, reduced penetration of cervical mucus by spermatozoa, and sterility in men. Acta Obstet Gynecol Scand. 1968;47(1):102–118. doi: 10.3109/00016346809157471. [DOI] [PubMed] [Google Scholar]
- Hekman A. Association of lactoferrin with other proteins, as demonstrated by changes in electrophoretic mobility. Biochim Biophys Acta. 1971 Dec 28;251(3):380–387. doi: 10.1016/0005-2795(71)90126-7. [DOI] [PubMed] [Google Scholar]
- Hekman A., Rümke P. The antigens of human seminal plasma. With special reference to lactoferrin as a spermatozoa-coating antigen. Fertil Steril. 1969 Mar-Apr;20(2):312–323. doi: 10.1016/s0015-0282(16)36973-4. [DOI] [PubMed] [Google Scholar]
- KARVONEN M. J., MALM M. Colorimetric determination of fructose with indol. Scand J Clin Lab Invest. 1955;7(4):305–307. doi: 10.3109/00365515509134669. [DOI] [PubMed] [Google Scholar]
- Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
- RUMKE P., HELLINGA G. Autoantibodies against spermatozoa in sterile men. Am J Clin Pathol. 1959 Oct;32:357–363. doi: 10.1093/ajcp/32.4.357. [DOI] [PubMed] [Google Scholar]
- Rümke P., Visser D., Kwa H. G., Hart A. A. Radio-immuno assay of lactoferrin in blood plasma of breast cancer patients, lactating and normal women; prevention of false high levels caused by leakage from neutrophile leucocytes in vitro. Folia Med Neerl. 1971 Aug-Sep;14(4):156–168. [PubMed] [Google Scholar]
- Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]
- Uehling D. T. Secretory IgA in seminal fluid. Fertil Steril. 1971 Nov;22(11):769–773. [PubMed] [Google Scholar]
- WILSON L. Sperm agglutinins in human semen and blood. Proc Soc Exp Biol Med. 1954 Apr;85(4):652–655. doi: 10.3181/00379727-85-20982. [DOI] [PubMed] [Google Scholar]