Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1991 Nov;86(2):271–277. doi: 10.1111/j.1365-2249.1991.tb05809.x

Sputum eosinophils from asthmatics express ICAM-1 and HLA-DR.

T T Hansel 1, J B Braunstein 1, C Walker 1, K Blaser 1, P L Bruijnzeel 1, J C Virchow Jr 1, C Virchow Sr 1
PMCID: PMC1554121  PMID: 1682072

Abstract

Sputum from symptomatic asthmatics is a rich source of eosinophils from the respiratory tract. Following liquefaction of sputum with dithioerythritol (DTE), a cell suspension for indirect double immunofluorescence with flow cytometry was obtained. Eosinophils were identified using anti-CD9 fluorescein conjugate, and particular surface markers measured with the relevant mouse MoAb followed by goat anti-mouse immunoglobulin phycoerythrin conjugate. Blood and sputum eosinophil surface markers were determined in parallel from asthmatics not receiving steroid therapy. Sputum eosinophils were found to have considerably elevated levels of CD11b, a reflection of eosinophil activation. Sputum but not blood eosinophils were found to express ICAM-1 (nine out of 11 cases) and HLA-DR (eight out of 11 cases). Furthermore, following culture of normal blood eosinophils with pooled T cell supernatants, ICAM-1 and HLA-DR could be induced in vitro. The induction of eosinophil adhesion molecules such as ICAM-1 and HLA-DR may influence eosinophil localization and function in asthma.

Full text

PDF
276

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albelda S. M., Buck C. A. Integrins and other cell adhesion molecules. FASEB J. 1990 Aug;4(11):2868–2880. [PubMed] [Google Scholar]
  2. Alfaro C., Sharma O. P., Navarro L., Glovsky M. M. Inverse correlation of expiratory lung flows and sputum eosinophils in status asthmaticus. Ann Allergy. 1989 Sep;63(3):251–254. [PubMed] [Google Scholar]
  3. Arnaout M. A. Structure and function of the leukocyte adhesion molecules CD11/CD18. Blood. 1990 Mar 1;75(5):1037–1050. [PubMed] [Google Scholar]
  4. Bousquet J., Chanez P., Lacoste J. Y., Barnéon G., Ghavanian N., Enander I., Venge P., Ahlstedt S., Simony-Lafontaine J., Godard P. Eosinophilic inflammation in asthma. N Engl J Med. 1990 Oct 11;323(15):1033–1039. doi: 10.1056/NEJM199010113231505. [DOI] [PubMed] [Google Scholar]
  5. Buckle A. M., Hogg N. The effect of IFN-gamma and colony-stimulating factors on the expression of neutrophil cell membrane receptors. J Immunol. 1989 Oct 1;143(7):2295–2301. [PubMed] [Google Scholar]
  6. CLELAND W. W. DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. Biochemistry. 1964 Apr;3:480–482. doi: 10.1021/bi00892a002. [DOI] [PubMed] [Google Scholar]
  7. Capron M., Jouault T., Prin L., Joseph M., Ameisen J. C., Butterworth A. E., Papin J. P., Kusnierz J. P., Capron A. Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages. J Exp Med. 1986 Jul 1;164(1):72–89. doi: 10.1084/jem.164.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Capron M., Kusnierz J. P., Prin L., Spiegelberg H. L., Ovlaque G., Gosset P., Tonnel A. B., Capron A. Cytophilic IgE on human blood and tissue eosinophils: detection by flow microfluorometry. J Immunol. 1985 May;134(5):3013–3018. [PubMed] [Google Scholar]
  9. Capron M., Spiegelberg H. L., Prin L., Bennich H., Butterworth A. E., Pierce R. J., Ouaissi M. A., Capron A. Role of IgE receptors in effector function of human eosinophils. J Immunol. 1984 Jan;132(1):462–468. [PubMed] [Google Scholar]
  10. Dustin M. L., Rothlein R., Bhan A. K., Dinarello C. A., Springer T. A. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J Immunol. 1986 Jul 1;137(1):245–254. [PubMed] [Google Scholar]
  11. Engleman E. G., Benike C. J., Grumet F. C., Evans R. L. Activation of human T lymphocyte subsets: helper and suppressor/cytotoxic T cells recognize and respond to distinct histocompatibility antigens. J Immunol. 1981 Nov;127(5):2124–2129. [PubMed] [Google Scholar]
  12. Frew A. J., Kay A. B. Eosinophils and T-lymphocytes in late-phase allergic reactions. J Allergy Clin Immunol. 1990 Mar;85(3):533–539. doi: 10.1016/0091-6749(90)90090-q. [DOI] [PubMed] [Google Scholar]
  13. Fukuda T., Dunnette S. L., Reed C. E., Ackerman S. J., Peters M. S., Gleich G. J. Increased numbers of hypodense eosinophils in the blood of patients with bronchial asthma. Am Rev Respir Dis. 1985 Nov;132(5):981–985. doi: 10.1164/arrd.1985.132.5.981. [DOI] [PubMed] [Google Scholar]
  14. Gibson P. G., Girgis-Gabardo A., Morris M. M., Mattoli S., Kay J. M., Dolovich J., Denburg J., Hargreave F. E. Cellular characteristics of sputum from patients with asthma and chronic bronchitis. Thorax. 1989 Sep;44(9):693–699. doi: 10.1136/thx.44.9.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gleich G. J. The eosinophil and bronchial asthma: current understanding. J Allergy Clin Immunol. 1990 Feb;85(2):422–436. doi: 10.1016/0091-6749(90)90151-s. [DOI] [PubMed] [Google Scholar]
  16. Khalife J., Capron M., Cesbron J. Y., Tai P. C., Taelman H., Prin L., Capron A. Role of specific IgE antibodies in peroxidase (EPO) release from human eosinophils. J Immunol. 1986 Sep 1;137(5):1659–1664. [PubMed] [Google Scholar]
  17. Koenderman L., Kok P. T., Hamelink M. L., Verhoeven A. J., Bruijnzeel P. L. An improved method for the isolation of eosinophilic granulocytes from peripheral blood of normal individuals. J Leukoc Biol. 1988 Aug;44(2):79–86. doi: 10.1002/jlb.44.2.79. [DOI] [PubMed] [Google Scholar]
  18. Kürzinger K., Reynolds T., Germain R. N., Davignon D., Martz E., Springer T. A. A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression, and structure. J Immunol. 1981 Aug;127(2):596–602. [PubMed] [Google Scholar]
  19. Lucey D. R., Nicholson-Weller A., Weller P. F. Mature human eosinophils have the capacity to express HLA-DR. Proc Natl Acad Sci U S A. 1989 Feb;86(4):1348–1351. doi: 10.1073/pnas.86.4.1348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Makgoba M. W., Sanders M. E., Ginther Luce G. E., Dustin M. L., Springer T. A., Clark E. A., Mannoni P., Shaw S. ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells. Nature. 1988 Jan 7;331(6151):86–88. doi: 10.1038/331086a0. [DOI] [PubMed] [Google Scholar]
  21. O'Shea J. J., Brown E. J., Seligmann B. E., Metcalf J. A., Frank M. M., Gallin J. I. Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophils. J Immunol. 1985 Apr;134(4):2580–2587. [PubMed] [Google Scholar]
  22. Rimmer E. F., Horton M. A. Origin of human mast cells studied by dual immunofluorescence. Clin Exp Immunol. 1987 Jun;68(3):712–718. [PMC free article] [PubMed] [Google Scholar]
  23. Shah R. R., Dye W. E. Use of dithiothreitol to replace n-acetyl-L-cysteine for routine sputum digestion-decontamination for the culture of mycobacteria. Am Rev Respir Dis. 1966 Sep;94(3):454–454. doi: 10.1164/arrd.1966.94.3.454. [DOI] [PubMed] [Google Scholar]
  24. Springer T. A. Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425–434. doi: 10.1038/346425a0. [DOI] [PubMed] [Google Scholar]
  25. Toews G. B., Vial W. C., Dunn M. M., Guzzetta P., Nunez G., Stastny P., Lipscomb M. F. The accessory cell function of human alveolar macrophages in specific T cell proliferation. J Immunol. 1984 Jan;132(1):181–186. [PubMed] [Google Scholar]
  26. Walsh G. M., Hartnell A., Moqbel R., Cromwell O., Nagy L., Bradley B., Furitsu T., Ishizaka T., Kay A. B. Receptor expression and functional status of cultured human eosinophils derived from umbilical cord blood mononuclear cells. Blood. 1990 Jul 1;76(1):105–111. [PubMed] [Google Scholar]
  27. Wegner C. D., Gundel R. H., Reilly P., Haynes N., Letts L. G., Rothlein R. Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science. 1990 Jan 26;247(4941):456–459. doi: 10.1126/science.1967851. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES