Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1991 Oct;86(1):179–184. doi: 10.1111/j.1365-2249.1991.tb05792.x

New monoclonal anti-human Fc gamma receptor II antibodies induced platelet aggregation

S Nomura, K Yamaguchi, H Kido, T Kawakatsu, K Iwata, T Fukuroi, M Suzuki, M Yanabu, T Soga, H Nagata, T Kokawa, K Yasunaga
PMCID: PMC1554145  PMID: 1833099

Abstract

We developed two new monoclonal antibodies, designated NNKY3-2 and NNKY4-7, that recognized a 40-kD platelet protein. They appeared to be monoclonal anti-Fc gamma receptor II (FcγRII) antibodies from the results of flow cytometric binding inhibition studies using another monoclonal anti-FcγRII antibody (2E1). The addition of NNKY3-2 or NNKY4-7 to platelet-rich plasma (PRP) led to a typical aggregation pattern preceded by a lag phase, but their addition to washed platelets did not induce aggregation. The aggregation of PRP by these antibodies was inhibited by prostaglandin E1 (PGE1) or staurosporine (protein kinase C inhibitor), whereas it was only slightly affected by a monoclonal anti-GPIIb/IIIa antibody or Arg—Gly—Asp—Ser. Furthermore, these antibodies induced the aggregation of washed platelets plus normal serum, but not that of washed platelets plus heat-treated serum (destruction of complement activity). These results suggest that NNKY3-2 or NNKY4-7-induced aggregation involves an unusual pathway independent of fibrinogen, and that the important factor is the participation of complement. NNKY3-2 and NNKY4-7 may be useful to study the relationship between autoantibodies, the Fc receptor, and complement in idiopathic thrombocytopenic purpura.

Full text

PDF
183

Images in this article

181Fig. 1
on p.181

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beardsley D. S., Spiegel J. E., Jacobs M. M., Handin R. I., Lux S. E., 4th Platelet membrane glycoprotein IIIa contains target antigens that bind anti-platelet antibodies in immune thrombocytopenias. J Clin Invest. 1984 Nov;74(5):1701–1707. doi: 10.1172/JCI111587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boucheix C., Soria C., Mirshahi M., Soria J., Perrot J. Y., Fournier N., Billard M., Rosenfeld C. Characteristics of platelet aggregation induced by the monoclonal antibody ALB6 (acute lymphoblastic leukemia antigen p 24). Inhibition of aggregation by ALB6Fab. FEBS Lett. 1983 Sep 19;161(2):289–295. doi: 10.1016/0014-5793(83)81027-8. [DOI] [PubMed] [Google Scholar]
  3. Farace F., Mitjavila M. T., Betaieb A., Dokhelar M. C., Wiels J., Finale Y., Kieffer N., Breton-Gorius J., Vainchenker W., Tursz T. New hematopoietic differentiation antigens detected by anti-K562 monoclonal antibodies. Cancer Res. 1988 Oct 15;48(20):5759–5765. [PubMed] [Google Scholar]
  4. Karpatkin S. Autoimmune thrombocytopenic purpura. Blood. 1980 Sep;56(3):329–343. [PubMed] [Google Scholar]
  5. Looney R. J., Abraham G. N., Anderson C. L. Human monocytes and U937 cells bear two distinct Fc receptors for IgG. J Immunol. 1986 Mar 1;136(5):1641–1647. [PubMed] [Google Scholar]
  6. McCrae K. R., Shattil S. J., Cines D. B. Platelet activation induces increased Fc gamma receptor expression. J Immunol. 1990 May 15;144(10):3920–3927. [PubMed] [Google Scholar]
  7. McMillan R. Chronic idiopathic thrombocytopenic purpura. N Engl J Med. 1981 May 7;304(19):1135–1147. doi: 10.1056/NEJM198105073041904. [DOI] [PubMed] [Google Scholar]
  8. Nomura S., Nagata H., Oda K., Kokawa T., Yasunaga K. Effects of EDTA on the membrane glycoproteins IIb-IIIa complex--analysis using flow cytometry. Thromb Res. 1987 Jul 1;47(1):47–58. doi: 10.1016/0049-3848(87)90239-8. [DOI] [PubMed] [Google Scholar]
  9. Panzer S., Szamait S., Bödeker R. H., Haas O. A., Haubenstock A., Mueller-Eckhardt C. Platelet-associated immunoglobulins IgG, IgM, IgA and complement C3 in immune and nonimmune thrombocytopenic disorders. Am J Hematol. 1986 Oct;23(2):89–99. doi: 10.1002/ajh.2830230203. [DOI] [PubMed] [Google Scholar]
  10. Peerschke E. I., Ghebrehiwet B. Human blood platelets possess specific binding sites for C1q. J Immunol. 1987 Mar 1;138(5):1537–1541. [PubMed] [Google Scholar]
  11. Peerschke E. I., Ghebrehiwet B. Modulation of platelet responses to collagen by Clq receptors. J Immunol. 1990 Jan 1;144(1):221–225. [PubMed] [Google Scholar]
  12. Ruan C. G., Du X. P., Xi X. D., Castaldi P. A., Berndt M. C. A murine antiglycoprotein Ib complex monoclonal antibody, SZ 2, inhibits platelet aggregation induced by both ristocetin and collagen. Blood. 1987 Feb;69(2):570–577. [PubMed] [Google Scholar]
  13. Talle M. A., Rao P. E., Westberg E., Allegar N., Makowski M., Mittler R. S., Goldstein G. Patterns of antigenic expression on human monocytes as defined by monoclonal antibodies. Cell Immunol. 1983 May;78(1):83–99. doi: 10.1016/0008-8749(83)90262-9. [DOI] [PubMed] [Google Scholar]
  14. Unkeless J. C. Function and heterogeneity of human Fc receptors for immunoglobulin G. J Clin Invest. 1989 Feb;83(2):355–361. doi: 10.1172/JCI113891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Worthington R. E., Carroll R. C., Boucheix C. Platelet activation by CD9 monoclonal antibodies is mediated by the Fc gamma II receptor. Br J Haematol. 1990 Feb;74(2):216–222. doi: 10.1111/j.1365-2141.1990.tb02568.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES