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. 1992 Jul;89(1):154–157. doi: 10.1111/j.1365-2249.1992.tb06895.x

Functionally active complement is present in human ovarian follicular fluid and can be activated by seminal plasma.

R Perricone 1, N Pasetto 1, C De Carolis 1, E Vaquero 1, E Piccione 1, L Baschieri 1, L Fontana 1
PMCID: PMC1554394  PMID: 1628423

Abstract

Human ovarian preovulatory follicular fluids (FF) from 10 women were analysed for their complement contents. Functionally active complement was detected in all the fluids studied in amounts similar to those present in normal human serum. Pooled FF was challenged by seminal plasma in order to determine whether seminal plasma could activate FF complement, the pattern of such an activation and the possible consequences on the reproductive function. FF complement activation occurred during the incubation with seminal plasma with features including alternative pathway activation, factor B and C3 conversion and reduction in total haemolytic complement, as well as an inhibition by seminal plasma of the FF complement response to a new activating challenge. Possible consequences for fertilization, implantation of a fertilized ovum and local defence mechanisms against viruses and bacteria are discussed.

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Selected References

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  1. Arroyave C. M., Tan E. M. Detection of complement activation by counterimmunoelectrophoresis (CIE). J Immunol Methods. 1976;13(2):101–112. doi: 10.1016/0022-1759(76)90148-4. [DOI] [PubMed] [Google Scholar]
  2. Arroyave C. M., Tan E. M. Mechanism of complement activation by radiographic contrast media. Clin Exp Immunol. 1977 Jul;29(1):89–94. [PMC free article] [PubMed] [Google Scholar]
  3. Castilla J. A., Molina R., Lopez-Nevot M. A., Vergara F., Garrido F., Herruzo A. J. Immunosuppressive properties of human follicular fluid. Fertil Steril. 1990 Feb;53(2):271–275. doi: 10.1016/s0015-0282(16)53280-4. [DOI] [PubMed] [Google Scholar]
  4. De Carolis C., De Sanctis G., Perricone R., Moretti C., Fabbri A., Gnessi L., Fraioli F., Fontana L. Evidence for an inhibitory role of beta-endorphin and other opioids on human total T rosette formation. Experientia. 1984 Jul 15;40(7):738–739. doi: 10.1007/BF01949751. [DOI] [PubMed] [Google Scholar]
  5. Droesch K., Fulgham D. L., Liu H. C., Rosenwaks Z., Alexander N. J. Distribution of T cell subsets in follicular fluid. Fertil Steril. 1988 Oct;50(4):618–621. [PubMed] [Google Scholar]
  6. Fabbri A., Gnessi L., Perricone R., De Sanctis G., Moretti C., De Carolis C., Fontana L., Isidori A., Fraioli F. Human semen inhibits T rosette formation through an opiate mediated mechanism. J Clin Endocrinol Metab. 1985 Apr;60(4):807–809. doi: 10.1210/jcem-60-4-807. [DOI] [PubMed] [Google Scholar]
  7. Fine D. P. Comparison of ethyleneglycoltetraacetic acid and its magnesium salt as reagents for studying alternative complement pathway function. Infect Immun. 1977 Apr;16(1):124–128. doi: 10.1128/iai.16.1.124-128.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fontana L., Perricone R., De Carolis C., Pizzolo J. G., Casciani C. U. Hereditary angioneurotic edema: clinical and laboratory findings in 58 subjects. Ric Clin Lab. 1989 Jan-Mar;19(1):51–58. doi: 10.1007/BF02871792. [DOI] [PubMed] [Google Scholar]
  9. Herriot D. M., Warnes G. M., Kerin J. F. Pregnancy-related chemotactic activity of human follicular fluid. Fertil Steril. 1986 Feb;45(2):196–201. doi: 10.1016/s0015-0282(16)49154-5. [DOI] [PubMed] [Google Scholar]
  10. Hess E. V., Marcus Z. H. The inhibitors in seminal plasma. J Lab Clin Med. 1980 Oct;96(4):577–581. [PubMed] [Google Scholar]
  11. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  12. Martin A., Lachmann P. J., Halbwachs L., Hobart M. J. Haemolytic diffusion plate assays for factors B and D of the alternative pathway of complement activation. Immunochemistry. 1976 Apr;13(4):317–324. doi: 10.1016/0019-2791(76)90341-4. [DOI] [PubMed] [Google Scholar]
  13. Perricone R., De Carolis C., Grimaldi O., Fontana L. Activation of human complement by crude and purified antigens from Echinococcus granulosus. Ric Clin Lab. 1984 Jan-Mar;14(1):65–72. doi: 10.1007/BF02905042. [DOI] [PubMed] [Google Scholar]
  14. Perricone R., Fontana L., de Carolis C., Carini C., Sirianni M. C., Aiuti F. Evidence for activation of complement in patients with AIDS related complex (ARC) and/or lymphoadenopathy syndrome (LAS). Clin Exp Immunol. 1987 Dec;70(3):500–507. [PMC free article] [PubMed] [Google Scholar]
  15. Perricone R., de Carolis C., Moretti C., Santuari E., de Sanctis G., Fontana L. Complement, complement activation and anaphylatoxins in human ovarian follicular fluid. Clin Exp Immunol. 1990 Nov;82(2):359–362. doi: 10.1111/j.1365-2249.1990.tb05453.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Petersen B. H., Lammel C. J., Stites D. P., Brooks G. F. Human seminal plasma inhibition of complement. J Lab Clin Med. 1980 Oct;96(4):582–591. [PubMed] [Google Scholar]
  17. Platts-Mills T. A., Ishizaka K. Activation of the alternate pathway of human complements by rabbit cells. J Immunol. 1974 Jul;113(1):348–358. [PubMed] [Google Scholar]
  18. Price R. J., Boettcher B. The presence of complement in human cervical mucus and its possible relevance to infertility in women with complement-dependent sperm-immobilizing antibodies. Fertil Steril. 1979 Jul;32(1):61–66. doi: 10.1016/s0015-0282(16)44117-8. [DOI] [PubMed] [Google Scholar]
  19. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  20. Takada Y., Arimoto Y., Mineda H., Takada A. Inhibition of the classical and alternative pathways by amino acids and their derivatives. Immunology. 1978 Mar;34(3):509–515. [PMC free article] [PubMed] [Google Scholar]

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