Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1992 Jul;89(1):58–62. doi: 10.1111/j.1365-2249.1992.tb06877.x

Circulating intercellular adhesion molecule-1 (ICAM-1) antigen in sera of patients with idiopathic pulmonary fibrosis

N Shijubo, K Imai, S Aoki, M Hirasawa, H Sugawara, H Koba, M Tsujisaki, T Sugiyama, Y Hinoda, A Yachi, M Asakawa, A Suzuki
PMCID: PMC1554395  PMID: 1352747

Abstract

Intercellular adhesion molecule-1 (ICAM-1), a member of immunoglobulin supergene family with a five-domain structure, is known to play an important role in inflammatory diseases. An ELISA was developed using two MoAbs against human ICAM-1 in order to detect the soluble shedding ICAM-1 antigen in sera. We measured levels of circulating ICAM-1 antigen in sera of patients with idiopathic pulmonary fibrosis (IPF), pulmonary sarcoidosis, hypersensitive pneumonitis, bacterial and mycoplasmal pneumonia, and inflammatory diseases of other organs. The results clearly demonstrated that IPF had significantly high levels of circulating ICAM-1 in sera as compared with other disorders or normal controls. Moreover, immunohistochemical analysis with MoAb against human ICAM-1 disclosed that in IPF, the expression of ICAM-1 was intensively enhanced on alveolar epithelial cells. These results suggest that ICAM-1 may contribute to the pathogenesis of IPF.

Full text

PDF
58

Images in this article

61Fig. 3
on p.61

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albelda S. M. Endothelial and epithelial cell adhesion molecules. Am J Respir Cell Mol Biol. 1991 Mar;4(3):195–203. doi: 10.1165/ajrcmb/4.3.195. [DOI] [PubMed] [Google Scholar]
  2. Ban T., Imai K., Yachi A. Immunohistological and immunochemical characterization of a novel pancreatic cancer-associated antigen MUSE11. Cancer Res. 1989 Dec 15;49(24 Pt 1):7141–7146. [PubMed] [Google Scholar]
  3. Barton R. W., Rothlein R., Ksiazek J., Kennedy C. The effect of anti-intercellular adhesion molecule-1 on phorbol-ester-induced rabbit lung inflammation. J Immunol. 1989 Aug 15;143(4):1278–1282. [PubMed] [Google Scholar]
  4. Crystal R. G., Bitterman P. B., Rennard S. I., Hance A. J., Keogh B. A. Interstitial lung diseases of unknown cause. Disorders characterized by chronic inflammation of the lower respiratory tract (first of two parts). N Engl J Med. 1984 Jan 19;310(3):154–166. doi: 10.1056/NEJM198401193100304. [DOI] [PubMed] [Google Scholar]
  5. Crystal R. G., Fulmer J. D., Roberts W. C., Moss M. L., Line B. R., Reynolds H. Y. Idiopathic pulmonary fibrosis. Clinical, histologic, radiographic, physiologic, scintigraphic, cytologic, and biochemical aspects. Ann Intern Med. 1976 Dec;85(6):769–788. doi: 10.7326/0003-4819-85-6-769. [DOI] [PubMed] [Google Scholar]
  6. Crystal R. G., Gadek J. E., Ferrans V. J., Fulmer J. D., Line B. R., Hunninghake G. W. Interstitial lung disease: current concepts of pathogenesis, staging and therapy. Am J Med. 1981 Mar;70(3):542–568. doi: 10.1016/0002-9343(81)90577-5. [DOI] [PubMed] [Google Scholar]
  7. Dayer J. M., de Rochemonteix B., Burrus B., Demczuk S., Dinarello C. A. Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells. J Clin Invest. 1986 Feb;77(2):645–648. doi: 10.1172/JCI112350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dustin M. L., Rothlein R., Bhan A. K., Dinarello C. A., Springer T. A. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J Immunol. 1986 Jul 1;137(1):245–254. [PubMed] [Google Scholar]
  9. Dustin M. L., Staunton D. E., Springer T. A. Supergene families meet in the immune system. Immunol Today. 1988 Jul-Aug;9(7-8):213–215. doi: 10.1016/0167-5699(88)91216-9. [DOI] [PubMed] [Google Scholar]
  10. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  11. Fulmer J. D., Roberts W. C., von Gal E. R., Crystal R. G. Morphologic-physiologic correlates of the severity of fibrosis and degree of cellularity in idiopathic pulmonary fibrosis. J Clin Invest. 1979 Apr;63(4):665–676. doi: 10.1172/JCI109349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Guesdon J. L., Ternynck T., Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979 Aug;27(8):1131–1139. doi: 10.1177/27.8.90074. [DOI] [PubMed] [Google Scholar]
  13. Hartmann D. P., Georgian M. M., Oghiso Y., Kagan E. Enhanced interleukin activity following asbestos inhalation. Clin Exp Immunol. 1984 Mar;55(3):643–650. [PMC free article] [PubMed] [Google Scholar]
  14. Hunninghake G. W., Gadek J. E., Kawanami O., Ferrans V. J., Crystal R. G. Inflammatory and immune processes in the human lung in health and disease: evaluation by bronchoalveolar lavage. Am J Pathol. 1979 Oct;97(1):149–206. [PMC free article] [PubMed] [Google Scholar]
  15. Keogh B. A., Crystal R. G. Alveolitis: the key to the interstitial lung disorders. Thorax. 1982 Jan;37(1):1–10. doi: 10.1136/thx.37.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koizumi M., Uede T., Shijubo N., Kudo R., Hashimoto M., Kikuchi K. New monoclonal antibody, 1C5, reactive with human cervical adenocarcinoma of the uterus, with immunodiagnostic potential. Cancer Res. 1988 Nov 15;48(22):6565–6572. [PubMed] [Google Scholar]
  17. Kuhn C., 3rd, Boldt J., King T. E., Jr, Crouch E., Vartio T., McDonald J. A. An immunohistochemical study of architectural remodeling and connective tissue synthesis in pulmonary fibrosis. Am Rev Respir Dis. 1989 Dec;140(6):1693–1703. doi: 10.1164/ajrccm/140.6.1693. [DOI] [PubMed] [Google Scholar]
  18. Maio M., Tessitori G., Pinto A., Temponi M., Colombatti A., Ferrone S. Differential role of distinct determinants of intercellular adhesion molecule-1 in immunologic phenomena. J Immunol. 1989 Jul 1;143(1):181–188. [PubMed] [Google Scholar]
  19. Makgoba M. W., Sanders M. E., Ginther Luce G. E., Dustin M. L., Springer T. A., Clark E. A., Mannoni P., Shaw S. ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells. Nature. 1988 Jan 7;331(6151):86–88. doi: 10.1038/331086a0. [DOI] [PubMed] [Google Scholar]
  20. Marlin S. D., Springer T. A. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell. 1987 Dec 4;51(5):813–819. doi: 10.1016/0092-8674(87)90104-8. [DOI] [PubMed] [Google Scholar]
  21. Müller N. L., Miller R. R., Webb W. R., Evans K. G., Ostrow D. N. Fibrosing alveolitis: CT-pathologic correlation. Radiology. 1986 Sep;160(3):585–588. doi: 10.1148/radiology.160.3.3737898. [DOI] [PubMed] [Google Scholar]
  22. Piguet P. F., Collart M. A., Grau G. E., Kapanci Y., Vassalli P. Tumor necrosis factor/cachectin plays a key role in bleomycin-induced pneumopathy and fibrosis. J Exp Med. 1989 Sep 1;170(3):655–663. doi: 10.1084/jem.170.3.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rothlein R., Czajkowski M., O'Neill M. M., Marlin S. D., Mainolfi E., Merluzzi V. J. Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies. J Immunol. 1988 Sep 1;141(5):1665–1669. [PubMed] [Google Scholar]
  24. Russo C., Callegaro L., Lanza E., Ferrone S. Re.: Purification of IgG monoclonal antibody by caprylic acid precipitation. J Immunol Methods. 1983 Dec 16;65(1-2):269–271. doi: 10.1016/0022-1759(83)90324-1. [DOI] [PubMed] [Google Scholar]
  25. Tsujisaki M., Imai K., Hirata H., Hanzawa Y., Masuya J., Nakano T., Sugiyama T., Matsui M., Hinoda Y., Yachi A. Detection of circulating intercellular adhesion molecule-1 antigen in malignant diseases. Clin Exp Immunol. 1991 Jul;85(1):3–8. doi: 10.1111/j.1365-2249.1991.tb05673.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wegner C. D., Gundel R. H., Reilly P., Haynes N., Letts L. G., Rothlein R. Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science. 1990 Jan 26;247(4941):456–459. doi: 10.1126/science.1967851. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES