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. 1993 Sep;93(3):452–455. doi: 10.1111/j.1365-2249.1993.tb08200.x

IgG inhibits the increase of platelet-associated C3 stimulated by anti-platelet antibodies

S Nomura, Y Miyazaki, T Miyake, K Yamaguchi, H Kido, T Kawakatsu, T Fukuroi, H Kagawa, M Suzuki, M Yanabu, T Kokawa
PMCID: PMC1554901  PMID: 8370175

Abstract

We investigated the increase of platelet-associated IgG and complement component 3 (C3) caused by the in vitro action of anti-platelet MoAbs, and the effect of mouse and human IgG on these events. Anti-glycoprotein IIb/IIIa and anti-glycoprotein Ib MoAbs caused a slight increase of C3, but not of platelet-associated IgG. In contrast, anti-CD9 and anti-Fcγ II receptor MoAbs caused an increase of both platelet-associated C3 and IgG. In particular, three MoAbs which activated the complement system caused a marked increase of C3. When platelet-rich plasma was treated with aspirin and prostaglandin E1 before incubation with antibodies, the increase of platelet-associated IgG was inhibited in all cases. In contrast, the increase of platelet-associated C3 was scarcely influenced. These results suggest that the binding to platelets of platelet-activating antibodies caused the increase expression of IgG molecules on the platelet surface and a possible increase of platelet-associated IgG. However, the increase of platelet-associated C3 appeared to depend on specific characteristics of the antibodies tested, such as a complement-activating effect. In addition, intact mouse or human IgG inhibited the increase of platelet-associated C3 caused by complement-activating antibodies, while F(ab')2 mouse or human IgG had no such effect. This suggested that the Fc portion of IgG may block the increase of C3 mediated by anti-platelet antibodies.

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Selected References

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  1. Arnott J., Horsewood P., Kelton J. G. Measurement of platelet-associated IgG in animal models of immune and nonimmune thrombocytopenia. Blood. 1987 May;69(5):1294–1299. [PubMed] [Google Scholar]
  2. Basta M., Fries L. F., Frank M. M. High doses of intravenous Ig inhibit in vitro uptake of C4 fragments onto sensitized erythrocytes. Blood. 1991 Jan 15;77(2):376–380. [PubMed] [Google Scholar]
  3. Basta M., Kirshbom P., Frank M. M., Fries L. F. Mechanism of therapeutic effect of high-dose intravenous immunoglobulin. Attenuation of acute, complement-dependent immune damage in a guinea pig model. J Clin Invest. 1989 Dec;84(6):1974–1981. doi: 10.1172/JCI114387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Basta M., Langlois P. F., Marques M., Frank M. M., Fries L. F. High-dose intravenous immunoglobulin modifies complement-mediated in vivo clearance. Blood. 1989 Jul;74(1):326–333. [PubMed] [Google Scholar]
  5. Bussel J. B., Pham L. C., Aledort L., Nachman R. Maintenance treatment of adults with chronic refractory immune thrombocytopenic purpura using repeated intravenous infusions of gammaglobulin. Blood. 1988 Jul;72(1):121–127. [PubMed] [Google Scholar]
  6. Capel P. J., Groeneboer O., Grosveld G., Pondman K. W. The binding of activated C3 to polysaccharides and immunoglobulins. J Immunol. 1978 Dec;121(6):2566–2572. [PubMed] [Google Scholar]
  7. Cines D. B., Schreiber A. D. Immune thrombocytopenia. Use of a Coombs antiglobulin test to detect IgG and C3 on platelets. N Engl J Med. 1979 Jan 18;300(3):106–111. doi: 10.1056/NEJM197901183000302. [DOI] [PubMed] [Google Scholar]
  8. Cines D. B., Wilson S. B., Tomaski A., Schreiber A. D. Platelet antibodies of the IgM class in immune thrombocytopenic purpura. J Clin Invest. 1985 Apr;75(4):1183–1190. doi: 10.1172/JCI111814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dixon R., Rosse W., Ebbert L. Quantitative determination of antibody in idiopathic thrombocytopenic purpura. Correlation of serum and platelet-bound antibody with clinical response. N Engl J Med. 1975 Jan 30;292(5):230–236. doi: 10.1056/NEJM197501302920503. [DOI] [PubMed] [Google Scholar]
  10. George J. N. Platelet immunoglobulin G: its significance for the evaluation of thrombocytopenia and for understanding the origin of alpha-granule proteins. Blood. 1990 Sep 1;76(5):859–870. [PubMed] [Google Scholar]
  11. Imbach P., Barandun S., d'Apuzzo V., Baumgartner C., Hirt A., Morell A., Rossi E., Schöni M., Vest M., Wagner H. P. High-dose intravenous gammaglobulin for idiopathic thrombocytopenic purpura in childhood. Lancet. 1981 Jun 6;1(8232):1228–1231. doi: 10.1016/s0140-6736(81)92400-4. [DOI] [PubMed] [Google Scholar]
  12. Karpatkin S. Autoimmune thrombocytopenic purpura. Blood. 1980 Sep;56(3):329–343. [PubMed] [Google Scholar]
  13. Kayser W., Mueller-Eckhardt C., Bhakdi S., Ebert K. Platelet-associated complement C3 in thrombocytopenic states. Br J Haematol. 1983 Jul;54(3):353–363. doi: 10.1111/j.1365-2141.1983.tb02110.x. [DOI] [PubMed] [Google Scholar]
  14. McMillan R. Chronic idiopathic thrombocytopenic purpura. N Engl J Med. 1981 May 7;304(19):1135–1147. doi: 10.1056/NEJM198105073041904. [DOI] [PubMed] [Google Scholar]
  15. NIH conference. Pathophysiology of immune hemolytic anemia. Ann Intern Med. 1977 Aug;87(2):210–222. doi: 10.7326/0003-4819-87-2-210. [DOI] [PubMed] [Google Scholar]
  16. Nomura S., Nagata H., Oda K., Kokawa T., Yasunaga K. Effects of EDTA on the membrane glycoproteins IIb-IIIa complex--analysis using flow cytometry. Thromb Res. 1987 Jul 1;47(1):47–58. doi: 10.1016/0049-3848(87)90239-8. [DOI] [PubMed] [Google Scholar]
  17. Nomura S., Nagata H., Suzuki M., Kondo K., Ohga S., Kawakatsu T., Kido H., Fukuori T., Yamaguchi K., Iwata K. Microparticle generation during in vitro platelet activation by anti-CD9 murine monoclonal antibodies. Thromb Res. 1991 Jun 1;62(5):429–439. doi: 10.1016/0049-3848(91)90016-p. [DOI] [PubMed] [Google Scholar]
  18. Nomura S., Suzuki M., Kido H., Yamaguchi K., Fukuroi T., Yanabu M., Soga T., Nagata H., Kokawa T., Yasunaga K. Differences between platelet and microparticle glycoprotein IIb/IIIa. Cytometry. 1992;13(6):621–629. doi: 10.1002/cyto.990130610. [DOI] [PubMed] [Google Scholar]
  19. Nomura S., Yamaguchi K., Kido H., Kawakatsu T., Iwata K., Fukuroi T., Suzuki M., Yanabu M., Soga T., Nagata H. New monoclonal anti-human Fc gamma receptor II antibodies induce platelet aggregation. Clin Exp Immunol. 1991 Oct;86(1):179–184. doi: 10.1111/j.1365-2249.1991.tb05792.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nomura S., Yanabu M., Fukuroi T., Kido H., Kawakatsu T., Yamaguchi K., Suzuki M., Kokawa T., Yasunaga K. Anti-glycoprotein IIb/IIIa autoantibodies are reversibly internalized into platelets in idiopathic (autoimmune) thrombocytopenic purpura. Autoimmunity. 1992;13(2):133–140. doi: 10.3109/08916939209001914. [DOI] [PubMed] [Google Scholar]
  21. Nomura S., Yanabu M., Kido H., Fukuroi T., Yamaguchi K., Soga T., Nagata H., Kokawa T., Yasunaga K. Antiplatelet autoantibody-related microparticles in patients with idiopathic (autoimmune) thrombocytopenic purpura. Ann Hematol. 1991 Apr;62(4):103–107. doi: 10.1007/BF01702922. [DOI] [PubMed] [Google Scholar]
  22. Oyaizu N., Yasumizu R., Miyama-Inaba M., Nomura S., Yoshida H., Miyawaki S., Shibata Y., Mitsuoka S., Yasunaga K., Morii S. (NZW x BXSB)F1 mouse. A new animal model of idiopathic thrombocytopenic purpura. J Exp Med. 1988 Jun 1;167(6):2017–2022. doi: 10.1084/jem.167.6.2017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Panzer S., Szamait S., Bödeker R. H., Haas O. A., Haubenstock A., Mueller-Eckhardt C. Platelet-associated immunoglobulins IgG, IgM, IgA and complement C3 in immune and nonimmune thrombocytopenic disorders. Am J Hematol. 1986 Oct;23(2):89–99. doi: 10.1002/ajh.2830230203. [DOI] [PubMed] [Google Scholar]

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