Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1981 Jun;43(2):219–226.

Autoantibody responses to liver-specific lipoprotein in mice.

W N Bartholomaeus, W D Reed, R A Joske, K B Shilkin
PMCID: PMC1555006  PMID: 7019049

Abstract

Autoantibody to the hepatocyte membrane antigen, liver-specific lipoprotein (LSP) was induced in mice by immunization with LSP-containing protein preparations from human, rat, rabbit and mouse liver and also with purified allogeneic LSP. Each of the strains of mice used (C57B1, BALB/c, C3H) showed the capacity to produce high titre autoantibody to LSP. Autoantibody to LSP demonstrated by passive haemagglutination was absorbed by normal mouse hepatocytes but not by kidney or spleen cells and reacted with the cell membrane of normal mouse hepatocytes by immunofluorescence. The liver was examined histologically in all mice and where inflammation was found it was attributable to the Freund's complete adjuvant used in immunization rather than liver protein immunogen. The demonstration of high titre autoantibody to LSP in mice without associated hepatitis contrasts with chronic hepatitis in man and experimental chronic hepatitis in rabbits where autoantibodies to LSP have been implicated in the pathogenesis of the disease.

Full text

PDF
220

Images in this article

223Figure 6
on p.223

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Behrens U. J., Paronetto F. Studies on "liver-specific" antigens. I. Evaluation of the liver specificity of "LSP" and "LP-2". Gastroenterology. 1979 Nov;77(5):1045–1052. [PubMed] [Google Scholar]
  2. Büschenfelde K. H., Kössling F. K., Miescher P. A. Experimental chronic active hepatitis in rabbits following immunization with human liver proteins. Clin Exp Immunol. 1972 May;11(1):99–108. [PMC free article] [PubMed] [Google Scholar]
  3. Cochrane A. M., Moussouros A., Smith A., Portmann B., Eddleston A. L., Williams R. Lymphocyte cytotoxicity in chronic active hepatitis: effect of therapy and correlations with clinical and histological changes. Gut. 1978 Apr;19(4):308–314. doi: 10.1136/gut.19.4.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cox K. O., Koh L. Y. Disappearance of IgG2B autoantibodies associated with recovery from anaemia. Clin Exp Immunol. 1977 Mar;27(3):560–563. [PMC free article] [PubMed] [Google Scholar]
  5. Farram E., Festenstein H. Mechanisms of "cytostasis" of tumours in vitro by syngeneic lymphoid cells of tumour bearers. Cell Immunol. 1979 May;44(2):352–366. doi: 10.1016/0008-8749(79)90011-x. [DOI] [PubMed] [Google Scholar]
  6. Ferluga J., Allison A. C. Role of mononuclear infiltrating cells in pathogenesis of hepatitis. Lancet. 1978 Sep 16;2(8090):610–611. doi: 10.1016/s0140-6736(78)92828-3. [DOI] [PubMed] [Google Scholar]
  7. Gonzales C., Cochrane A. M., Eddleston A. L., Williams R. Mechanisms responsible for antibody-dependent, cell-mediated cytotoxicity to isolated hepatocytes in chronic active hepatitis. Gut. 1979 May;20(5):385–388. doi: 10.1136/gut.20.5.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Greenberg A. H., Shen L., Medley G. Characteristics of the effector cells mediating cytotoxicity against antibody-coated target cells. I. Phagocytic and non-phagocytic effector cell activity against erythrocyte and tumour target cells in a 51Cr release cytotoxicity assay and [125I]IUdR growth inhibition assay. Immunology. 1975 Oct;29(4):719–729. [PMC free article] [PubMed] [Google Scholar]
  9. Greenberg A. H., Shen L., Roitt I. M. Characterization of the antibody-dependent cytotoxic cell. A non-phagocytic monocyte? Clin Exp Immunol. 1973 Oct;15(2):251–259. [PMC free article] [PubMed] [Google Scholar]
  10. Hopf U., Meyer zum Büschenfelde K. H., Arnold W. Detection of a liver-membrane autoantibody in HBsAg-negative chronic active hepatitis. N Engl J Med. 1976 Mar 11;294(11):578–582. doi: 10.1056/NEJM197603112941103. [DOI] [PubMed] [Google Scholar]
  11. Hopf U., Meyer zum Büschenfelde K. H., Freudenberg J. Liver-specific antigens of different species. II. Localization of a membrane antigen at cell surface of isolated hepatocytes. Clin Exp Immunol. 1974 Jan;16(1):117–123. [PMC free article] [PubMed] [Google Scholar]
  12. Jensen D. M., McFarlane I. G., Portmann B. S., Eddleston A. L., Williams R. Detection of antibodies directed against a liver-specific membrane lipoprotein in patients with acute and chronic active hepatitis. N Engl J Med. 1978 Jul 6;299(1):1–7. doi: 10.1056/NEJM197807062990101. [DOI] [PubMed] [Google Scholar]
  13. McFarlane I. G., Wojcicka B. M., Zucker G. M., Eddleston A. L., Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol. 1977 Mar;27(3):381–390. [PMC free article] [PubMed] [Google Scholar]
  14. Meyer zum Büschenfelde K. H., Hopf U. Studies on the pathogenesis of experimental chronic active hepatitis in rabbits. I. Induction of the disease and protective effect of allogeneic liver specific proteins. Br J Exp Pathol. 1974 Oct;55(5):498–508. [PMC free article] [PubMed] [Google Scholar]
  15. Smith C. I., Cooksley W. G., Powell L. W. Cell-mediated immunity to liver antigen in toxic liver injury. I. Occurrence and specificity. Clin Exp Immunol. 1980 Mar;39(3):607–617. [PMC free article] [PubMed] [Google Scholar]
  16. Smith C. I., Cooksley W. G., Powell L. W. Cell-mediated immunity to liver antigen in toxic liver injury. II. Role in pathogenesis of liver damage. Clin Exp Immunol. 1980 Mar;39(3):618–625. [PMC free article] [PubMed] [Google Scholar]
  17. Spiegelberg H. L., Perlmann H., Perlmann P. Interaction of K lymphocytes with myeloma proteins of different IgG subclasses. J Immunol. 1976 Nov;117(5 Pt 1):1464–1471. [PubMed] [Google Scholar]
  18. Thomson A. D., Cochrane M. A., McFarlane I. G., Eddleston A. L., Williams R. Lymphocyte cytotoxicity to isolated hepatocytes in chronic active hepatitis. Nature. 1974 Dec 20;252(5485):721–722. doi: 10.1038/252721a0. [DOI] [PubMed] [Google Scholar]
  19. Warnatz H., Scheiffarth F., Schmeissner R. Studies on the cytotoxic effect of in vivo and in vitro immunized lymphocytes on liver target cells. Clin Exp Immunol. 1975 Aug;21(2):250–258. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES