Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1981 Jun;43(2):227–234.

IgE antibody suppression following aerosol exposure to antigens.

P C Fox, R P Siraganian
PMCID: PMC1555028  PMID: 7251053

Abstract

Exposure of mice to aerosolized antigens induced a low level IgG1 response but not detectable IgE antibodies. Subsequent intraperitoneal immunization of these mice demonstrated immunoglobulin class-specific IgE suppression. Low concentrations of nebulized antigen induced IgE suppression which was antigen specific and persisted on subsequent secondary and tertiary injections. Although a single aerosolized antigen exposure significantly decreased the IgE response, maximal suppression was observed when the mice were exposed to nebulized antigen once weekly for at least 6 weeks. The suppression was not observed until 3 weeks following nebulizer exposure. Mice exposed once weekly to nebulized antigen for 6 weeks and then rested for 2 months before intraperitoneal immunization still demonstrated suppression. However, animals first immunized intraperitoneally and then exposed to nebulized antigen produced normal secondary IgE and IgG1 responses. These results suggest that antigen exposure by aerosol may profoundly alter the IgE response.

Full text

PDF
230

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. André C., Heremans J. F., Vaerman J. P., Cambiaso C. L. A mechanism for the induction of immunological tolerance by antigen feeding: antigen-antibody complexes. J Exp Med. 1975 Dec 1;142(6):1509–1519. doi: 10.1084/jem.142.6.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barboriak J. J., Knoblock H. W., Hensley G. T., Gombas O. F., Fink J. N. Animal model of sensitization by inhalation. Clin Exp Immunol. 1976 Jun;24(3):542–545. [PMC free article] [PubMed] [Google Scholar]
  3. Chalon M. P., Milne R. W., Vaerman J. P. In vitro immunosuppressive effect of serum from orally immunized mice. Eur J Immunol. 1979 Oct;9(10):747–751. doi: 10.1002/eji.1830091002. [DOI] [PubMed] [Google Scholar]
  4. David M. F. Prevention of homocytotropic antibody formation and anaphylactic sensitization by prefeeding antigen. J Allergy Clin Immunol. 1977 Sep;60(3):180–187. doi: 10.1016/0091-6749(77)90122-1. [DOI] [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  6. Hanson D. G., Vaz N. M., Maia L. C., Lynch J. M. Inhibition of specific immune responses by feeding protein antigens. III. Evidence against maintenance of tolerance to ovalbumin by orally induced antibodies. J Immunol. 1979 Nov;123(5):2337–2343. [PubMed] [Google Scholar]
  7. Ishizaka K., Okudaira H. Reaginic antibody formation in the mouse. I. Antibody-mediated suppression of reaginic antibody formation. J Immunol. 1972 Jul;109(1):84–89. [PubMed] [Google Scholar]
  8. Katz D. H. The allergic phenotype: manifestation of 'allergic breakthrough' and imbalance in normal 'damping' of IgE antibody production. Immunol Rev. 1978;41:77–108. doi: 10.1111/j.1600-065x.1978.tb01461.x. [DOI] [PubMed] [Google Scholar]
  9. Lee W. Y., Sehon A. H. Suppression of reaginic antibodies. Immunol Rev. 1978;41:200–247. doi: 10.1111/j.1600-065x.1978.tb01466.x. [DOI] [PubMed] [Google Scholar]
  10. Ngan J., Kind L. S. Suppressor T cells for IgE and IgG in Peyer's patches of mice made tolerant by the oral administration of ovalbumin. J Immunol. 1978 Mar;120(3):861–865. [PubMed] [Google Scholar]
  11. Tada T. Regulation of reaginic antibody formation in animals. Prog Allergy. 1975;19:122–194. [PubMed] [Google Scholar]
  12. Takatsu K., Ishizaka K. Reaginic antibody formation in the mouse. VII. Depression of the ongoing IgE antibody formation by suppressor T cells. J Immunol. 1976 Oct;117(4):1211–1218. [PubMed] [Google Scholar]
  13. Van Hout C. A., Johnson H. G. Synthesis of rat IgE by aerosol immunization. J Immunol. 1972 Mar;108(3):834–836. [PubMed] [Google Scholar]
  14. Watanabe N., Kojima S., Shen F. W., Ovary Z. Suppression of IgE antibody production in SJL mice. II. Expression of Ly-1 antigen on helper and nonspecific suppressor T cells. J Immunol. 1977 Feb;118(2):485–488. [PubMed] [Google Scholar]
  15. Willoughby J. B., Willoughby W. F. In vivo responses to inhaled proteins. I. Quantitative analysis of antigen uptake, fate, and immunogenicity in a rabbit model system. J Immunol. 1977 Dec;119(6):2137–2146. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES