Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1981 Jul;43(3):547–554.

Acquired immunity to Salmonella typhimurium and delayed (footpad) hypersensitivity in BALB/c mice.

C E Hormaeche, M C Fahrenkrog, R A Pettifor, J Brock
PMCID: PMC1555054  PMID: 7019057

Abstract

BALB/c mice are extremely susceptible to salmonella infections. Previous reports have suggested that this natural susceptibility is due to a defect in cell-mediated immunity (CMI) which correlates with their inability to develop a delayed (footpad) hypersensitivity reaction to a salmonella extract when immunized with attenuated salmonellae. We have shown that mice thus immunized are in fact highly resistant to superinfecting intravenous challenge with virulent organisms, at a time when the footpad test is still negative. The footpad test becomes positive 2-3 weeks later, after the appearance of CMI, which is already present at 1 week as measured by determining the fate of a superinfecting challenge in the RES. The positive footpad reactions that develop in BALB/c mice--and also in B10, and CBA and (B10XA/J)F1 mice--are transferable to normal recipients by thetasensitive spleen cells. However, although B10 mice give positive delayed hypersensitivity (DH) reactions, they are more susceptible to salmonellae of intermediate virulence than the DH negative BALB/c strain. We have also shown that previous reports which suggested that susceptible mice did not develop immunity when vaccinated with live organisms are probably due to the salmonella strain used for vaccination, which does not establish a carrier state. A strain which does establish a carrier state effectively immunizes the susceptible BALB/c strain against virulent challenge, indicating that natural susceptibility does not preclude the development of acquired immunity to reinfection. X

Full text

PDF
554

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradley D. J., Taylor B. A., Blackwell J., Evans E. P., Freeman J. Regulation of Leishmania populations within the host. III. Mapping of the locus controlling susceptibility to visceral leishmaniasis in the mouse. Clin Exp Immunol. 1979 Jul;37(1):7–14. [PMC free article] [PubMed] [Google Scholar]
  2. Cohen A., Schlesinger M. Absorption of guinea pig serum with agar. A method for elimination of itscytotoxicity for murine thymus cells. Transplantation. 1970 Jul;10(1):130–132. doi: 10.1097/00007890-197007000-00027. [DOI] [PubMed] [Google Scholar]
  3. Collins F. M. Effect of adjuvant on immunogenicity of a heat-killed salmonella vaccine. J Infect Dis. 1972 Jul;126(1):69–76. doi: 10.1093/infdis/126.1.69. [DOI] [PubMed] [Google Scholar]
  4. Collins F. M., Mackaness G. B. Delayed hypersensitivity and arthus reactivity in relation to host resistance in salmonella-infected mice. J Immunol. 1968 Nov;101(5):830–845. [PubMed] [Google Scholar]
  5. Collins F. M. Mechanisms in antimicrobial immunity. J Reticuloendothel Soc. 1971 Jul;10(1):58–99. [PubMed] [Google Scholar]
  6. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davidson W. F., Parish C. R. A procedure for removing red cells and dead cells from lymphoid cell suspensions. J Immunol Methods. 1975 Jun;7(2-3):291–300. doi: 10.1016/0022-1759(75)90026-5. [DOI] [PubMed] [Google Scholar]
  8. GOWEN J. W. Genetic effects in nonspecific resistance to infectious disease. Bacteriol Rev. 1960 Mar;24(1):192–200. doi: 10.1128/br.24.1.192-200.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hormaeche C. E. Genetics of natural resistance to salmonellae in mice. Immunology. 1979 Jun;37(2):319–327. [PMC free article] [PubMed] [Google Scholar]
  10. Hormaeche C. E. Natural resistance to Salmonella typhimurium in different inbred mouse strains. Immunology. 1979 Jun;37(2):311–318. [PMC free article] [PubMed] [Google Scholar]
  11. Hormaeche C. E., Pettifor R. A., Brock J. The fate of temperature-sensitive salmonella mutants in vivo in naturally resistant and susceptible mice. Immunology. 1981 Apr;42(4):569–576. [PMC free article] [PubMed] [Google Scholar]
  12. Hormaeche C. E. The in vivo division and death rates of Salmonella typhimurium in the spleens of naturally resistant and susceptible mice measured by the superinfecting phage technique of Meynell. Immunology. 1980 Dec;41(4):973–979. [PMC free article] [PubMed] [Google Scholar]
  13. Hormaeche C. E. The natural resistance of radiation chimeras to S. typhimurium C5. Immunology. 1979 Jun;37(2):329–332. [PMC free article] [PubMed] [Google Scholar]
  14. JENKIN C. R., ROWLEY D., AUZINS I. THE BASIS FOR IMMUNITY TO MOUSE TYPHOID. I. THE CARRIER STATE. Aust J Exp Biol Med Sci. 1964 Apr;42:215–228. doi: 10.1038/icb.1964.23. [DOI] [PubMed] [Google Scholar]
  15. Maier T., Oels H. C. Role of the macrophage in natural resistance to salmonellosis in mice. Infect Immun. 1972 Oct;6(4):438–443. doi: 10.1128/iai.6.4.438-443.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Medina S., Vas S. I., Robson H. G. Effect of nonspecific stimulation on the defense mechanisms of inbred mice. J Immunol. 1975 Jun;114(6):1720–1725. [PubMed] [Google Scholar]
  17. O'Brien A. D., Rosenstreich D. L., Taylor B. A. Control of natural resistance to Salmonella typhimurium and Leishmania donovani in mice by closely linked but distinct genetic loci. Nature. 1980 Oct 2;287(5781):440–442. doi: 10.1038/287440a0. [DOI] [PubMed] [Google Scholar]
  18. Plant J., Glynn A. A. Genetics of resistance to infection with Salmonella typhimurium in mice. J Infect Dis. 1976 Jan;133(1):72–78. doi: 10.1093/infdis/133.1.72. [DOI] [PubMed] [Google Scholar]
  19. Plant J., Glynn A. A. Locating salmonella resistance gene on mouse chromosome 1. Clin Exp Immunol. 1979 Jul;37(1):1–6. [PMC free article] [PubMed] [Google Scholar]
  20. Plant J., Glynn A. A. Natural resistance to Salmonella infection, delayed hypersensitivity and Ir genes in different strains of mice. Nature. 1974 Mar 22;248(446):345–347. doi: 10.1038/248345a0. [DOI] [PubMed] [Google Scholar]
  21. Robson H. G., Vas S. I. Resistance of inbred mice to Salmonella typhimurium. J Infect Dis. 1972 Oct;126(4):378–386. doi: 10.1093/infdis/126.4.378. [DOI] [PubMed] [Google Scholar]
  22. Sjöberg O., Andersson J., Möller G. Requirement for adherent cells in the primary and secondary immune response in vitro. Eur J Immunol. 1972 Apr;2(2):123–126. doi: 10.1002/eji.1830020206. [DOI] [PubMed] [Google Scholar]
  23. von Jeney N., Günther E., Jann K. Mitogenic stimulation of murine spleen cells: relation to susceptibility to Salmonella infection. Infect Immun. 1977 Jan;15(1):26–33. doi: 10.1128/iai.15.1.26-33.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES