Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1981 May;43(1):81–89.

Natural killer cells appear to play no role in the recovery of mice from Sindbis virus infection.

R L Hirsch
PMCID: PMC1555197  PMID: 7251052

Abstract

Previous studies have suggested that non-specific defence mechanisms may be important in the development of age-dependent resistance to fatal Sindbis-virus infection and in the recovery of adult mice from non-fatal infection. In these studies, natural killer (NK) cell induction was studied in 7-day-old susceptible mice and 28-35-day-old resistant mice. It was found that Sindbis virus infection induced NK cells in both the young and older mice, suggesting that NK cells were not important in the acquisition of resistance to fatal Sindbis-virus infection. Transfer of 10(8) lymph node cells from adult, mice, at the peak of NK cell activity, did not protect young mice from fatal infections, supporting the in vitro findings. The pathogenesis of Sindbis virus infection in C57BL/6J bg/bg (NK-cell deficient) and bg/+ (NK-cell normal) mice was also studied. Despite a defect in the induction of NK cells by Sindbis virus infection in the bg/bg mice, there were no significant differences in the pathogenesis of either peripheral or intracerebral infection in these strains of mice. These studies suggest that although NK cells are induced, they may not be important in the recovery of mice from Sindbis virus infection.

Full text

PDF
87

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Golstein P., Blomgren H. Further evidence for autonomy of T cells mediating specific in vitro cytotoxicity: efficiency of very small amounts of highly purified T cells. Cell Immunol. 1973 Oct;9(1):127–141. doi: 10.1016/0008-8749(73)90174-3. [DOI] [PubMed] [Google Scholar]
  2. Griffin D. E. Role of the immune response in age-dependent resistance of mice to encephalitis due to Sindbis virus. J Infect Dis. 1976 Apr;133(4):456–464. doi: 10.1093/infdis/133.4.456. [DOI] [PubMed] [Google Scholar]
  3. Hackbarth S. A., Reinarz A. B., Sagik B. P. Age-dependent resistance of mice to sindbis virus infection: reticuloendothelial role. J Reticuloendothel Soc. 1973 Nov;14(5):405–425. [PubMed] [Google Scholar]
  4. Herberman R. B., Nunn M. E., Holden H. T., Lavrin D. H. Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells. Int J Cancer. 1975 Aug 15;16(2):230–239. doi: 10.1002/ijc.2910160205. [DOI] [PubMed] [Google Scholar]
  5. Herberman R. B., Nunn M. E., Lavrin D. H. Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic acid allogeneic tumors. I. Distribution of reactivity and specificity. Int J Cancer. 1975 Aug 15;16(2):216–229. doi: 10.1002/ijc.2910160204. [DOI] [PubMed] [Google Scholar]
  6. Hirsch R. L., Griffin D. E., Johnson R. T. Interactions between immune cells and antibody in protection from fatal Sindbis virus encephalitis. Infect Immun. 1979 Feb;23(2):320–324. doi: 10.1128/iai.23.2.320-324.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirsch R. L., Griffin D. E. The pathogenesis of Sindbis virus infection in athymic nude mice. J Immunol. 1979 Sep;123(3):1215–1218. [PubMed] [Google Scholar]
  8. Johnson R. T., McFarland H. F., Levy S. E. Age-dependent resistance to viral encephalitis: studies of infections due to Sindbis virus in mice. J Infect Dis. 1972 Mar;125(3):257–262. doi: 10.1093/infdis/125.3.257. [DOI] [PubMed] [Google Scholar]
  9. Kumagai K., Itoh K., Hinuma S., Tada M. Pretreatment of plastic Petri dishes with fetal calf serum. A simple method for macrophage isolation. J Immunol Methods. 1979;29(1):17–25. doi: 10.1016/0022-1759(79)90121-2. [DOI] [PubMed] [Google Scholar]
  10. Kärre K., Klein G. O., Kiessling R., Klein G., Roder J. C. Low natural in vivo resistance to syngeneic leukaemias in natural killer-deficient mice. Nature. 1980 Apr 17;284(5757):624–626. doi: 10.1038/284624a0. [DOI] [PubMed] [Google Scholar]
  11. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  12. Macfarlan R. I., Burns W. H., White D. O. Two cytotoxic cells in peritoneal cavity of virus-infected mice: antibody-dependent macrophages and nonspecific killer cells. J Immunol. 1977 Nov;119(5):1569–1574. [PubMed] [Google Scholar]
  13. Mattes M. J., Sharrow S. O., Herberman R. B., Holden H. T. Identification and separation of Thy-1 positive mouse spleen cells active in natural cytotoxicity and antibody-dependent cell-mediated cytotoxicity. J Immunol. 1979 Dec;123(6):2851–2860. [PubMed] [Google Scholar]
  14. McFarland H. F., Griffin D. E., Johnson R. T. Specificity of the inflammatory response in viral encephalitis. I. Adoptive immunization of immunosuppressed mice infected with Sindbis virus. J Exp Med. 1972 Aug 1;136(2):216–226. doi: 10.1084/jem.136.2.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reinarz A. B., Broome M. G., Sagik B. P. Age-dependent resistance of mice to sindbis virus infection: viral replication as a function of host age. Infect Immun. 1971 Feb;3(2):268–273. doi: 10.1128/iai.3.2.268-273.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Roder J. C. The beige mutation in the mouse. I. A stem cell predetermined impairment in natural killer cell function. J Immunol. 1979 Nov;123(5):2168–2173. [PubMed] [Google Scholar]
  17. Talmadge J. E., Meyers K. M., Prieur D. J., Starkey J. R. Role of NK cells in tumour growth and metastasis in beige mice. Nature. 1980 Apr 17;284(5757):622–624. doi: 10.1038/284622a0. [DOI] [PubMed] [Google Scholar]
  18. Welsh R. M., Jr Cytotoxic cells induced during lymphocytic choriomeningitis virus infection of mice. I. Characterization of natural killer cell induction. J Exp Med. 1978 Jul 1;148(1):163–181. doi: 10.1084/jem.148.1.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Welsh R. M., Jr, Hallenbeck L. A. Effect of virus infections on target cell susceptibility to natural killer cell-mediated lysis. J Immunol. 1980 May;124(5):2491–2497. [PubMed] [Google Scholar]
  20. Welsh R. M., Jr, Kiessling R. W. Natural killer cell response to lymphocytic choriomeningitis virus in beige mice. Scand J Immunol. 1980;11(4):363–367. doi: 10.1111/j.1365-3083.1980.tb00001.x. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES