Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1981 Oct;44(2):431–438.

Lymphocyte transformation induced by autologous cells. XI. The effect of age on the autologous mixed lymphocyte reaction.

C E Moody, J B Innes, L Staiano-Coico, G S Incefy, H T Thaler, M E Weksler
PMCID: PMC1555227  PMID: 6457793

Abstract

The autologous mixed lymphocyte reaction (MLR) was lower in newborn infants and healthy subjects over 65 years of age than in adults between the ages of 20 and 32. In contrast, the allogeneic MLR, although impaired in newborn infants, was normal in elderly subjects. The degree of impairment of the autologous MLR in elderly subjects was correlated with the impairment in the response of lymphocytes from elderly subjects to phytohaemagglutinin (PHA) and Staphylococcus aureus proteins A (SPA). The percentage of autorosetting T cells and of T cells with the OKT4 phenotype was increased in elderly subjects. These findings are paradoxical as autoreactive T cells in young adults have been reported to be drawn from these two T-cell subpopulations.

Full text

PDF
433

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Denman A. M., Barnes R. D. Cooperating and controlling functions of thymus-derived lymphocytes in relation to autoimmunity. Lancet. 1971 Jul 17;2(7716):135–140. doi: 10.1016/s0140-6736(71)92306-3. [DOI] [PubMed] [Google Scholar]
  2. DeKruyff R. H., Rinnooy Kan E. A., Weksler M. E., Siskind G. W. Effect of aging on T-cell tolerance induction. Cell Immunol. 1980 Nov;56(1):58–67. doi: 10.1016/0008-8749(80)90081-7. [DOI] [PubMed] [Google Scholar]
  3. Diaz-Jouanen E., Williams R. C., Jr, Strickland R. G. Letter: Age-related changes in T and B cells. Lancet. 1975 Mar 22;1(7908):688–689. doi: 10.1016/s0140-6736(75)91794-8. [DOI] [PubMed] [Google Scholar]
  4. Fernandez L. A., MacSween J. M. Decreased autologous mixed lymphocyte reaction with aging. Mech Ageing Dev. 1980 Mar;12(3):245–248. doi: 10.1016/0047-6374(80)90047-0. [DOI] [PubMed] [Google Scholar]
  5. Fournier C., Charreire J. Autologous mixed lymphocyte reaction in man. I. Relationship with age and sex. Cell Immunol. 1981 May 1;60(1):212–219. doi: 10.1016/0008-8749(81)90261-6. [DOI] [PubMed] [Google Scholar]
  6. Fournier C., Charreire J. Increase in autologous erythrocyte binding by T cells with ageing in man. Clin Exp Immunol. 1977 Sep;29(3):468–473. [PMC free article] [PubMed] [Google Scholar]
  7. Goidl E. A., Innes J. B., Weksler M. E. Immunological studies of aging. II. Loss of IgG and high avidity plaque-forming cells and increased suppressor cell activity in aging mice. J Exp Med. 1976 Oct 1;144(4):1037–1048. doi: 10.1084/jem.144.4.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gupta S., Good R. A. Subpopulations of human T lymphocytes. X. Alterations in T, B, third population cells, and T cells with receptors for immunoglobulin M (Tmu) or G (Tgamma) in aging humans. J Immunol. 1979 Apr;122(4):1214–1219. [PubMed] [Google Scholar]
  9. Hallgren H. M., Buckley C. E., 3rd, Gilbertsen V. A., Yunis E. J. Lymphocyte phytohemagglutinin responsiveness, immunoglobulins and autoantibodies in aging humans. J Immunol. 1973 Oct;111(4):1101–1107. [PubMed] [Google Scholar]
  10. Hausman P. B., Stobo J. D. Specificity and function of a human autologous reactive T cell. J Exp Med. 1979 Jun 1;149(6):1537–1542. doi: 10.1084/jem.149.6.1537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inkeles B., Innes J. B., Kuntz M. M., Kadish A. S., Weksler M. E. Immunological studies of Aging. III. Cytokinetic basis for the impaired response of lymphocytes from aged humans to plant lectins. J Exp Med. 1977 May 1;145(5):1176–1187. doi: 10.1084/jem.145.5.1176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Innes J. B., Kuntz M. M., Kim Y. T., Weksler M. E. Induction of suppressor activity in the autologous mixed lymphocyte reaction and in cultures with concanavalin A. J Clin Invest. 1979 Dec;64(6):1608–1613. doi: 10.1172/JCI109622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. James S. P., Elson C. O., Waggoner J. G., Jones E. A., Strober W. Deficiency of the autologous mixed lymphocyte reaction in patients with primary biliary cirrhosis. J Clin Invest. 1980 Dec;66(6):1305–1310. doi: 10.1172/JCI109982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuntz M. M., Innes J. B., Weksler M. E. The cellular basis of the impaired autologous mixed lymphocyte reaction in patients with systemic lupus erythematosus. J Clin Invest. 1979 Jan;63(1):151–153. doi: 10.1172/JCI109270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Makinodan T., Kay M. M. Age influence on the immune system. Adv Immunol. 1980;29:287–330. doi: 10.1016/s0065-2776(08)60047-4. [DOI] [PubMed] [Google Scholar]
  16. Miyasaka N., Sauvezie B., Pierce D. A., Daniels T. E., Talal N. Decreased autologous mixed lymphocyte reaction in Sjögren's syndrome. J Clin Invest. 1980 Nov;66(5):928–933. doi: 10.1172/JCI109960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moody C. E., Casazza B. A., Christenson W. N., Weksler M. E. Lymphocyte transformation induced by autologous cells. VIII. Impaired autologous mixed lymphocyte reactivity in patients with acute infectious mononucleosis. J Exp Med. 1979 Dec 1;150(6):1448–1455. doi: 10.1084/jem.150.6.1448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Palacios R., Llorente L., Alarcón-Segovia D., Ruíz-Arguelles A., Díaz-Jouanen E. Autologous rosette-forming T cells as the responding cells in human autologous mixed-lymphocyte reaction. J Clin Invest. 1980 Jun;65(6):1527–1530. doi: 10.1172/JCI109819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pisciotta A. V., Westring D. W., DePrey C., Walsh B. Mitogenic effect of phytohaemagglutinin at different ages. Nature. 1967 Jul 8;215(5097):193–194. doi: 10.1038/215193a0. [DOI] [PubMed] [Google Scholar]
  20. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2. J Immunol. 1980 Mar;124(3):1301–1307. [PubMed] [Google Scholar]
  21. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4061–4065. doi: 10.1073/pnas.76.8.4061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sakane T., Steinberg A. D., Green I. Failure of autologous mixed lymphocyte reactions between T and non-T cells in patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3464–3468. doi: 10.1073/pnas.75.7.3464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Steel C. M., Evans J., Smith M. A. The sheep-cell rosette: test on human peripheral blood lymyphocytes: an analysis of some analysis of some variable factors in the technique. Br J Haematol. 1974 Oct;28(2):245–251. doi: 10.1111/j.1365-2141.1974.tb06659.x. [DOI] [PubMed] [Google Scholar]
  24. Thomas Y., Sosman J., Irigoyen O., Friedman S. M., Kung P. C., Goldstein G., Chess L. Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative T-T interactions in the immunoregulation of B cell differentiation. J Immunol. 1980 Dec;125(6):2402–2408. [PubMed] [Google Scholar]
  25. Vande Stouwe R. A., Kunkel H. G., Halper J. P., Weksler M. E. Autologous mixed lymphocyte culture reactions and generation of cytotoxic T cells. J Exp Med. 1977 Dec 1;146(6):1809–1814. doi: 10.1084/jem.146.6.1809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weksler M. E., Hütteroth T. H. Impaired lymphocyte function in aged humans. J Clin Invest. 1974 Jan;53(1):99–104. doi: 10.1172/JCI107565. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES