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. 1982 Oct;47(2):283–288.

Analysis of antibodies in local and disseminated Neisseria gonorrhoeae infections by means of gel electrophoresis-derived ELISA.

S G Hadfield, A A Glynn
PMCID: PMC1555434  PMID: 6811421

Abstract

Major antigens in Neisseria gonorrhoeae were identified by surface labelling the organisms with 125I and electrophoresing extracts in polyacrylamide with sodium dodecyl sulphate. Horizontal slices of the gels were cut out and tested in individual wells against patients' sera using ELISA. Serum from local gonococcal infections reacted with Protein II and, probably, lipopolysaccharide, but not with Protein I in deoxycholate (DOC) extracts and gave no reaction with Triton X-100 extracts. Serum from disseminated gonococcal infections reacted with Protein I in the DOC extract and with pili and a number of undefined possibly cytoplasmic membrane antigens in the Triton X-100 extract. The significance of the results and the potential of the method are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  2. Glynn A. A., Ison C. Serological diagnosis of gonorrhoea by an enzyme-linked immunosorbent assay (Elisa). Br J Vener Dis. 1978 Apr;54(2):97–102. doi: 10.1136/sti.54.2.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Horikawa S., Ogawara H. A simple and rapid procedure for removal of triton X-100 from protein solution. Anal Biochem. 1979 Aug;97(1):116–119. doi: 10.1016/0003-2697(79)90334-8. [DOI] [PubMed] [Google Scholar]
  4. Ison C., Glynn A. A. Bulk growth of Neisseria gonorrhoeae type 1 in a biphasic system. Br J Vener Dis. 1978 Dec;54(6):394–397. doi: 10.1136/sti.54.6.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Lutz H., Higgins J., Pedersen N. C., Theilen G. H. The demonstration of antibody specificity by a new technique. The gel electrophoresis-derived enzyme-linked immunosorbent assay (GEDELISA) and its application to antibodies specific for feline leukemia virus. J Histochem Cytochem. 1979 Aug;27(8):1216–1218. doi: 10.1177/27.8.225379. [DOI] [PubMed] [Google Scholar]
  7. Markwell M. A., Fox C. F. Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. Biochemistry. 1978 Oct 31;17(22):4807–4817. doi: 10.1021/bi00615a031. [DOI] [PubMed] [Google Scholar]
  8. Smyth C. J., Friedman-Kien A. E., Salton M. R. Antigenic analysis of Neisseria gonorrhoeae by crossed immunoelectrophoresis. Infect Immun. 1976 Apr;13(4):1273–1288. doi: 10.1128/iai.13.4.1273-1288.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Swanson J. Studies on gonococcus infection. XII. Colony color and opacity varienats of gonococci. Infect Immun. 1978 Jan;19(1):320–331. doi: 10.1128/iai.19.1.320-331.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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