Skip to main content
Immunology logoLink to Immunology
. 1982 Oct;47(2):329–336.

Monoclonal antibodies (McAbs) to determinants on human gamma chains: properties of antibodies showing subclass restriction or subclass specificity.

J Lowe, P Bird, D Hardie, R Jefferis, N R Ling
PMCID: PMC1555448  PMID: 6180976

Abstract

Thirteen monoclonal antibodies (McAbs) showing subclass-specific and subclass-restricted reactivity to human IgG have been produced by the hybridoma technique. Their specificity has been defined by passive haemagglutination (HA) and haemagglutination-inhibition (HAI) assays using a large panel pf purified IgG paraproteins of known subclass. Their use in assaying human IgG subclasses in radial immunodiffusion (RID) and in purifying human subclasses by both positive and negative immunoabsorption chromatography is illustrated.

Full text

PDF
333

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cowing C., Garabedian C., Leskowitz S. Strain differences in tolerance induction to human gamma-globulin subclasses: dependence on macrophages. Cell Immunol. 1979 Oct;47(2):407–415. doi: 10.1016/0008-8749(79)90350-2. [DOI] [PubMed] [Google Scholar]
  2. Duhamel R. C., Schur P. H., Brendel K., Meezan E. pH gradient elution of human IgG1, IgG2 and IgG4 from protein A-sepharose. J Immunol Methods. 1979;31(3-4):211–217. doi: 10.1016/0022-1759(79)90133-9. [DOI] [PubMed] [Google Scholar]
  3. GREY H. M., KUNKEL H. G. H CHAIN SUBGROUPS OF MYELOMA PROTEINS AND NORMAL 7S GAMMA-GLOBULIN. J Exp Med. 1964 Aug 1;120:253–266. doi: 10.1084/jem.120.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  5. Hjelm H. Isolation of IgG3 from normal human sera and from a patient with multiple myeloma by using protein A-sepharose 4B. Scand J Immunol. 1975;4(7):633–640. doi: 10.1111/j.1365-3083.1975.tb02671.x. [DOI] [PubMed] [Google Scholar]
  6. Jefferis R., Weston P. D., Stanworth D. R., Clamp J. R. Relationship between the papain sensitivity of human gammaG immunoglobulins and their heavy chain subclass. Nature. 1968 Aug 10;219(5154):646–649. doi: 10.1038/219646b0. [DOI] [PubMed] [Google Scholar]
  7. Ling N. R., Lowe J., Jefferis R. Monoclonal antibodies to the idiotypic determinants of a human IgG paraprotein: evidence for complexity of the anti-idiotype response. Clin Exp Immunol. 1982 Apr;48(1):1–7. [PMC free article] [PubMed] [Google Scholar]
  8. Lowe J., Hardie D., Jefferis R., Ling N. R., Drysdale P., Richardson P., Raykundalia C., Catty D., Appleby P., Drew R. Properties of monoclonal antibodies to human immunoglobulin kappa and lambda chains. Immunology. 1981 Apr;42(4):649–659. [PMC free article] [PubMed] [Google Scholar]
  9. Michaelsen T. E., Frangione B., Franklin E. C. Primary structure of the "hinge" region of human IgG3. Probable quadruplication of a 15-amino acid residue basic unit. J Biol Chem. 1977 Feb 10;252(3):883–889. [PubMed] [Google Scholar]
  10. Natvig J. B., Kunkel H. G. Human immunoglobulins: classes, subclasses, genetic variants, and idiotypes. Adv Immunol. 1973;16:1–59. doi: 10.1016/s0065-2776(08)60295-3. [DOI] [PubMed] [Google Scholar]
  11. Partridge L. J., Lowe J., Hardie D. L., Ling N. R., Jefferis R. Immunogenic and antigenic epitopes of immunoglobulins. II. Antigenic differences between secreted and membrane IgG demonstrated using monoclonal antibodies. J Immunol. 1982 Jan;128(1):1–6. [PubMed] [Google Scholar]
  12. Schur P. H. Human gamma-g subclasses. Prog Clin Immunol. 1972;1:71–104. [PubMed] [Google Scholar]
  13. Spiegelberg H. L., Weigle W. O. The production of antisera to human gammaGlobulin subclasses in rabbits using immunological unresponsiveness. J Immunol. 1968 Aug;101(2):377–380. [PubMed] [Google Scholar]
  14. TERRY W. D., FAHEY J. L. SUBCLASSES OF HUMAN GAMMA-2-GLOBULIN BASED ON DIFFERENCES IN THE HEAVY POLYPEPTIDE CHAINS. Science. 1964 Oct 16;146(3642):400–401. doi: 10.1126/science.146.3642.400. [DOI] [PubMed] [Google Scholar]
  15. Wang A. C., Tung E., Fudenberg H. H. The primary structure of a human IgG2 heavy chain: genetic, evolutionary, and functional implications. J Immunol. 1980 Sep;125(3):1048–1054. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES